MatrigelBD, Un Andamiaje Biocompatible que Estimula la Proliferación de Celulas Troncales Mesenquimales Derivadas del Tejido Gingival.

MatrigelBD is a hydrogel scaffold with three-dimensional intercrossed networks of hydrophilic polymers with high water content. Human gingival tissue might represent a better source of MSCs, allowing these cells to be easily obtained in a relatively non-invasive way. The objective of this study was to evaluate the biocompatibility of MatrigelBD with GMSCs in vitro. Gingival connective tissue samples were obtained from healthy donors. Fresh tissue was minced and cultured during two weeks, after which cells at passage fourth were analyzed for their immune phenotype by flow cytometry. Differentiation into osteogenic, chondrogenic, and adipogenic lineages was induced and evaluated by culture staining. The "construct" was made of MatrigelBD with GMSC. To assess the biocompatibility, an MTT cellular proliferation assay was performed. The differentiation potential of the cells toward the osteogenic, adipogenic, and chondrogenic lineages was analyzed after 21 days of growth in MatrigelBD with induction differentiation media. The MTT analysis showed that MatrigelBD stimulated cell proliferation; the GMSCs maintained the expression of MSC markers. Importantly, the growth of GMSCs within the MatrigelBD did not interfere with the cell differentiation potential. These findings indicate that MatrigelBD is biocompatible with GMSCs, and this matrix improves cell proliferation in vitro.publishedVersio

Search for high mass gamma gamma resonances in e(+)e(-)->l(+)l(-)gamma gamma, nu(nu)over-bar-gamma gamma and q(q)over-bar-gamma gamma at LEP I

A search for high mass photon pairs from the processes e(+)e(-) –> l(+)l(-)gamma gamma, e(+)e(-) –> <q(q)over bar gamma gamma> and e(+)e(-) –> nu nu gamma gamma with the DELPHI detector at LEP I is reported. From a data sample containing 3.5 million hadronic Z(0) decays, collected by DELPHI during the years 1991 to 1994, 79 events with two charged leptons and two isolated photons were selected with photon pair masses above 10 GeV/c(2), where 76 +/- 6 events were predicted from standard sources. In the same data sample, no <nu(nu)over bar gamma gamma> candidates were found and no accumulation of events was visible for gamma gamma masses above 10 GeVlc2 in the q<q(q)over bar gamma gamma> channel. Upper limits at 95% confidence level on the Z(0) branching ratios for the three different channels were extracted from the data. In the mass region m(gamma gamma) > 30 CeV/c(2) the limits obtained are between 3x10(-6) and 4x10(-6)