Suplementação dietária de pacu (Piaractus mesopotamicus) com vitamina C: respostas fisiológicas, imunidade não específica e resistência a inoculação com Aeromonas hydrophila

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Partial substitution of fishmeal with soybean protein-based diets for dourado Salminus brasiliensis

Cost and environmental restrictions limit the use of fishmeal (FM) in aquafeeds and plant protein (PP) sources. Soybean by-products are important alternative; however, their antinutritional factors may reduce fish growth. This study evaluates the effect of FM substitution for PP (soybean meal and soy protein concentrate) in diets of dourado (Salminus brasiliensis), a promising species for fish farming and empirically considered highly tolerant to PP-based diets. A control diet was formulated to contain 20 % of fishmeal and 61 % of plant protein (SBM) and other five diets with decreasing FM graded levels (16 %, 12 %, 8 %, 4 %, 0 %), which were replaced by soybean protein concentrate (SPC), comprising six treatments and four replicates. Fifteen dourados (6.73 ± 0.2 g) were stocked into 200 L tanks in a recirculating system, fed with experimental diets three times a day to apparent satiation for 90 days. Growth performance, food conversion ratio, protein efficiency ratio and protein retention efficiency of fish were impaired when FM substitution exceeded 13.05 % (the broken line analysis method). Histological analysis of intestinal mucosa showed a decrease in villus height when fishmeal was totally replaced by PP, which probably impaired digestive functions of fish and reflected in a severe loss on growth performance

FIR & IAW auf griechisch? Institut für Qualität und Arbeitswissenschaft in Athen geplant

The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the hemolytic activity of the proteins of the complement system (alternative pathway) increased after the pathogen challenge, but was not influenced by the β-glucan treatment