Phytotherapeutical Methods Used by Traditional Healers of Eturnagaram Mandal, Warangal, Andhra Pradesh, India

The indigenous traditional practices of Koyas (Dorasattamu) of Tupakulagudem, Bhupathipuram and Devadula villages which are on the south of Godavari river, Eturnagaram mandal warangal District, Andhrapradesh, India are being here reported. They use different parts of medicinal plants for curing common ailments. The ethnomedicinal information includes 32 plant species, useful parts of plants and mode of administration

Micropropagation of Crotalaria laburnifolia L. – An ethnomedicinally important herbal species

A protocol for in vitro shoot multiplication in Crotalaria labumifolia L. through nodal explants was established. Excision and culture of the nodal segments from in vitro developed shoots on fresh MS medium with concentration of BA (1mg/1) facilitated development of multiple shoots. Subsequent cultures enhanced the rate of shoot proliferation. Shoots cultured on ½ MS medium containing NAA (0.5 mg/1) initiated roots well compared with IBA and this is the most suitable protocol studied in C. labumifolia. The present study is the first report on in vitro regeneration in this species&nbsp

A study on Seed germination of Cassia alata Linn an antiallergenic plant

Cassia alata known as candle bush or Ringworm tree belongs to Cesalpinaceae family, which was found in diverse habitats in the tropics. C. alata leaves containing some chemical substances like chrysophanic acid, is a common ingredient in soaps, shampoos and lotions. The effectiveness of this plant against skin diseases is confirmed by modern scientific studies. The seed germination was gradually decreased by increasing the age of the seeds. Seed propagation is still used as a specialized tool for breeding purposes and for the propagation of pathogen-free plant material

Micropropagation of <i>Stachytarpheta jamaicensis</i> (L.) Vahl—A medicinally important plant

105-107An in vitro-protocol for micropropagation of Stachytarpheta jamaicensis (L.) Vahl was developed through nodal explants. Murashige and Skoog (MS) medium with BA 0.5 mg/L and IBA 0.5mg/L was found to be the best for multiple shoot formation from nodal explants. The maximum response of shoot induction was observed on a medium containing 1.0 mg/L Kn. The regenerated shoots were excised and transferred to rooting medium containing IBA at different concentrations. Highest of 84% rooting was observed at 0.5 mg/L level of IBA and BA. The in vitro derived plantlets were hardened and acclimatized in soil. About 75% of plantlets survived in the field condition

De novo synthesis of 2,2-<i>bis</i>(dimethylamino)-3-alkyl or benzyl 2,3-dihydroquinazolin-4(1<i>H</i>)-one compounds

<p>A new versatile and efficient strategy for the synthesis of 2,2-<i>bis</i>(dimethylamino)-3-alkyl or benzyl 2,3-dihydroquinazoline-4(1<i>H</i>)-one compounds has been developed by one-pot multicomponent reaction with isatoic anhydride, amines followed by <i>in situ</i>-generated Vilsmeier reagent. The reaction has also been studied with different amines and solvents.</p

Identification and testing of novel CARP-1 functional mimetic compounds as inhibitors of non-small cell lung and triple negative breast cancers

The triple negative breast cancer (TNBCs) and non-small cell lung cancers (NSCLCs) often acquire mutations that contribute to failure of drugs in clinic and poor prognosis, thus presenting an urgent need to develop new and improved therapeutic modalities. Here we report that CARP-1 functional mimetic (CFMs) compounds 4 and 5, and 4.6, a structurally related analog of CFM-4, are potent inhibitors of TNBC and NSCLC cells in vitro. Cell growth suppression by CFM-4 and -4.6 involved interaction and elevated expression of CARP-1/CCAR1 and Death Effector Domain (DED) containing DNA binding (DEDD)2 proteins. Apoptosis by these compounds also involved activation of pro-apoptotic stress-activated kinases p38 and JNK1/2, cleavage of PARP and loss of mitotic cyclin B1. Both the CFMs inhibited abilities of NSCLC and TNBC cells to migrate, invade, and form colonies in suspension, while disrupting tubule formation by the human umbilical vein endothelial cells (HUVECs). Nano-lipid formulation of CFM-4 (CFM-4 NLF) enhanced its serum bioavailability when compared with the free CFM-4. Oral administration of CFM-4 NLF reduced weights and volume of the xenografted tumors derived from A549 NSCLC and MDA-MB-231 TNBC cells. Although no gross tissue or histological toxicities were noticed, the immuno-histochemical analysis revealed increased CARP-1 and DNA fragmentation in tumors of the CFM-4 NLF-treated animals. In conclusion, while stimulation of pro-apoptotic CARP-1 and DEDD2 expression and their binding underscore a novel mechanism of apoptosis transduction by CFM compounds, our proof-of-concept xenograft studies demonstrate therapeutic potential of CFM-4 for TNBC and NSCLC