TLC determination of some flavanones in the buds of different genus Populus species and hybrids

Flavonoids in the buds of eight Populus species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid aglycones belonging to different groups, namely, flavones, flavonols, flavanones and flavanonols, previously described as constituents of poplar buds, was performed on silica gel plates using a hexane/ethyl acetate/formic acid (60:40:1.3, V/V/V) mixture as the mobile phase. Pinocembrin and pinostrobin were found in the majority of analyzed poplar buds. For quantitative analysis of both compounds, two TLC evaluation modes, densitometric and videodensitometric, were compared and the established methods were validated. Concentrations of flavanones in some extracts differed slightly or significantly due to the analyzed plant matrix complexity and the TLC evaluation mode applied. Poplar buds rich in flavanones originated from P. canadensis ‘Robusta’ (1.82 and 2.23 g per 100 g, resp.) and P. balsamifera (1.17 and 2.24 g per 100 g, resp.)

TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum

This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was validated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4’-O-glucoside and quercetin-3,4’-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 (‘Exihibition’) to 1079 mg kg–1 fresh mass (fm) (‘Hybing’) in edible parts, and from 1727 (‘Hyline’) to 28949 mg kg–1 fm (‘Red Baron’) in outer scales. The bulbs of two shallot cultivars contained 209 (‘Ambition’) and 523 mg kg–1 fm (‘Matador’) of flavonols in edible parts and 5426 and 8916 mg kg–1 fm in outer scales, respectively

Zastosowanie metod chromatograficznych w badanich składu chemicznego kory niektórych gatunków i klonów wierzby

Praca wykonana w: Katedra i Zakład Farmakognozji, Ogród Roślin Leczniczych

TLC determination of some flavanones in the buds of different genus Populus species and hybrids

Flavonoids in the buds of eight Populus species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid aglycones belonging to different groups, namely, flavones, flavonols, flavanones and flavanonols, previously described as constituents of poplar buds, was performed on silica gel plates using a hexane/ethyl acetate/formic acid (60:40:1.3, V/V/V) mixture as the mobile phase. Pinocembrin and pinostrobin were found in the majority of analyzed poplar buds. For quantitative analysis of both compounds, two TLC evaluation modes, densitometric and videodensitometric, were compared and the established methods were validated. Concentrations of flavanones in some extracts differed slightly or significantly due to the analyzed plant matrix complexity and the TLC evaluation mode applied. Poplar buds rich in flavanones originated from P. × canadensis ‘Robusta’ (1.82 and 2.23 g per 100 g, resp.) and P. balsamifera (1.17 and 2.24 g per 100 g, resp.)

HPLC-DAD-ESI/MS comparison of the chemical composition of flowers from two Arnica species grown in Poland

Introduction: Arnica flowers are used in pharmaceutical and cosmetic industry. According to EMA only endangered Arnica montana provides the medicinal plant material. However, some European countries also allow the use of A. chamissonis flowers, whose chemical composition is not known in detail

HPLC-DAD-ESI/MS Analysis of Arnica TM Constituents

The herbal drugs obtained from Arnica montana flowers are known for their anti-inflammatory activity. However, there is no detailed data on chemical constituents of the whole Arnica plant. HPLC-DAD-ESI/MS analysis of the whole Arnica montana plant (Arnica TM), used in the preparation of allopathic and homeopathic medicinal products, has been conducted. The qualitative and quantitative analysis comprised phenolic acids, flavonoids and sesquiterpene lactones – helenalin and dihydrohelenaline esters identified earlier in Arnica flowers. The analyzed material showed to be a rich source of caffeoylquinic acid (CQA) derivatives, especially 3,5-di-O-caffeoylquinic acid. According to the literature data, anti-inflammatory activity of extracts from the whole Arnica montana plant can be due to CQA presence as dominating compounds

TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum

This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4′-O-glucoside and quercetin-3,4′-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 (‘Exihibition’) to 1079 mg kg-1 fresh mass (fm) (‘Hybing’) in edible parts, and from 1727 (‘Hyline’) to 28949 mg kg-1 fm (‘Red Baron’) in outer scales. The bulbs of two shallot cultivars contained 209 (‘Ambition’) and 523 mg kg-1 fm (‘Matador’) of flavonols in edible parts and 5426 and 8916 mg kg-1 fm in outer scales, respectively

Chemosystematic Value of the Essential Oil Composition of Thuja species Cultivated in Poland—Antimicrobial Activity

In the framework of the correlation between chemotaxonomy and chemical analysis studies, the chemical composition of the essential oils of four varieties of Thuja species cultivated in Poland − T. occidentalis ‘globosa’, T. occidentalis ‘aurea’, T. plicata and T. plicata ‘gracialis’ − were investigated by GC and GC-MS. Thirty-one compounds were identified from T. occidentalis ‘globosa’, representing 96.92% of the total oil; twenty-seven from T. occidentalis ‘aurea’ (94.34%); thirty-one from T. plicata (94.75%); and thirty compounds from T. plicata ‘gracialis’ (96.36%). The main constituents in all samples were the monoterpene ketones α- and β-thujone, fenchone and sabinene, as well as the diterpenes beyerene and rimuene.The chemosystematic value of the total ketone content of all samples (which varied from 54.30–69.18%) has been discussed and investigated. The constituents, beyerene and the mixture of α- and β-thujone, were isolated from the oils and tested against six Gram-positive and -negative bacteria and three pathogenic fungi. The oils of the two T. plicata species exhibited significant antimicrobial activity, while the mixture of α- and β-thujone showed very strong activity as well

Chemosystematic value of essential oil compostion of Thuja species cultivated in Poland— Antimicrobial activity

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