Molecular identification of the samples SPS1, MGS1, MGS2, MGS3, SES1, SES2, and SES3 using the nuclear <i>tpm1</i> (a) and mitochondrial <i>mt-co1</i> (b) genes in multiplex-PCR.

<p>Lanes 1–12, genotypes of hybrid tambacu (♀ tambaqui x ♂ pacu); and lanes 13, 14, and 15, control DNA samples from the pure pacu, tambaqui, and pirapitinga species, respectively; M, 1 Kb Plus DNA Ladder.</p

Schematic representation of the backcrossing between ♂ tambaqui x ♀ patinga (♀ pacu x ♂ pirapitinga), demonstrating the <i>tpm1</i> and <i>rag2</i> gene loci and the expected probability of each genotype.

<p>Schematic representation of the backcrossing between ♂ tambaqui x ♀ patinga (♀ pacu x ♂ pirapitinga), demonstrating the <i>tpm1</i> and <i>rag2</i> gene loci and the expected probability of each genotype.</p

Sizes of the PCR products or restriction fragments, according to Hashimoto et al. [12].

<p>Sizes of the PCR products or restriction fragments, according to Hashimoto et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089902#pone.0089902-Hashimoto3" target="_blank">[12]</a>.</p

Schematic representation of the events of crossing-over and the fusion/retention of the second polar body, which likely generated the triploid individual of genotype E.

<p>Schematic representation of the events of crossing-over and the fusion/retention of the second polar body, which likely generated the triploid individual of genotype E.</p

Metaphases and the respective karyotypes of individuals of the SPS2 stock, showing the chromosome location of the 5S rRNA clusters.

<p>The arrows and arrowheads indicate the 5S rRNA major and minor clusters, respectively, in metaphase. In the karyotypes, the green bars indicate the chromosomes bearing 5S rRNA genes and their respective locations (long or short arms). (a) an individual of genotype A, (b) an individual of genotype B, and (c) an individual of genotype E.</p

Molecular identification of the SPS2 stock using the nuclear <i>tpm1</i> (a) and <i>rag2</i> (b) genes, and mitochondrial <i>mt-co1</i> (c) and <i>mt-cyb</i> (d) genes.

<p>Lanes 4, 11, and 12, genotype A; lane 5, genotype B; lanes 3, 6, and 7, genotype C; lanes 1, 2, 9, and 10, genotype D; lane 8, genotype E; and lanes 13, 14, and 15, control DNA samples from the pure pacu, tambaqui, and pirapitinga species, respectively; M, 1 Kb Plus DNA Ladder.</p

Table_S1

Comparative analysis of turbot microsatellites with Senegalese sole (Solea senegalensis), bastard halibut (Paralichthys olivaceus) and Atlantic halibut (Hippoglossus hippoglossus)

Table_1

Summary of the the technical characteristics of the 83 polymorphic microsatellite loci validated from the turbot (Scophthalmus maximus) EST database

Characidium filtered RADtags

Fasta file containing the sequences of the 9,863 final filtered Radtags (biallelic SNPs with a minimum depth of 10 reads and genotyped in at least 28 individuals

GP_513NeutralSNP_wild_turbot

Genepop input file created by Populations. Only markers included in neutral dataset (513 markers). Location codes are shown in Table 1 of the manuscript