CALHM1 Deletion in Mice Affects Glossopharyngeal Taste Responses, Food Intake, Body Weight, and Life Span

Stimulation of Type II taste receptor cells (TRCs) with T1R taste receptors causes sweet or umami taste, whereas T2Rs elicit bitter taste. Type II TRCs contain the calcium channel, calcium homeostasis modulator protein 1 (CALHM1), which releases adenosine triphosphate (ATP) transmitter to taste fibers. We have previously demonstrated with chorda tympani nerve recordings and two-bottle preference (TBP) tests that mice with genetically deleted Calhm1 (knockout [KO]) have severely impaired perception of sweet, bitter, and umami compounds, whereas their sour and salty tasting ability is unaltered. Here, we present data from KO mice of effects on glossopharyngeal (NG) nerve responses, TBP, food intake, body weight, and life span. KO mice have no NG response to sweet and a suppressed response to bitter compared with control (wild-type [WT]) mice. KO mice showed some NG response to umami, suggesting that umami taste involves both CALHM1- and non-CALHM1-modulated signals. NG responses to sour and salty were not significantly different between KO and WT mice. Behavioral data conformed in general with the NG data. Adult KO mice consumed less food, weighed significantly less, and lived almost a year longer than WT mice. Taken together, these data demonstrate that sweet taste majorly influences food intake, body weight, and life span

CALHM1 Deletion in Mice Affects Glossopharyngeal Taste Responses, Food Intake, Body Weight, and Life Span

Stimulation of Type II taste receptor cells (TRCs) with T1R taste receptors causes sweet or umami taste, whereas T2Rs elicit bitter taste. Type II TRCs contain the calcium channel, calcium homeostasis modulator protein 1 (CALHM1), which releases adenosine triphosphate (ATP) transmitter to taste fibers. We have previously demonstrated with chorda tympani nerve recordings and two-bottle preference (TBP) tests that mice with genetically deleted Calhm1 (knockout [KO]) have severely impaired perception of sweet, bitter, and umami compounds, whereas their sour and salty tasting ability is unaltered. Here, we present data from KO mice of effects on glossopharyngeal (NG) nerve responses, TBP, food intake, body weight, and life span. KO mice have no NG response to sweet and a suppressed response to bitter compared with control (wild-type [WT]) mice. KO mice showed some NG response to umami, suggesting that umami taste involves both CALHM1- and non-CALHM1-modulated signals. NG responses to sour and salty were not significantly different between KO and WT mice. Behavioral data conformed in general with the NG data. Adult KO mice consumed less food, weighed significantly less, and lived almost a year longer than WT mice. Taken together, these data demonstrate that sweet taste majorly influences food intake, body weight, and life span

Einsatz der Pheromonverwirrungstechnik zur Regulierung der Rosskastanien-Miniermotte - Cameraria ohridella Deschka u. Dimic (Lep.: Gracillariidae)

The horse chestnut leafminer is an introduced invasive pest that every year causes extensive damage to the leaves of the white flowering horse chestnut tree. An environmentally friendly method to control insect pests involves the use of sex pheromones for mating disruption. A large quantity of artificial pheromone is released into the insect plant system confusing males to the extend that they are unable to locate receptive females. In order to test the potential efficacy of the mating disruption technique on the horse chestnut leafminer we set up semi-field trials with closed experimental units and varying leafminer densities.We counted the number of leaf mines on pheromone treated horse chestnut trees and compared them with an untreated control. Mating disruption lead to a significant reduction in damage by more than 90% in the first and second generation of the leafminer, irrespective of the initial leafminer density. Although these results are very encouraging, high population densities of the horse chestnut leafminer in nature and the patchy distribution of horse chestnut trees in the cities will challenge the efficacy of mating disruption in the field. Further experiments are plannedwith open experimental units and typical horse chestnut trees in various parks and gardens.Gitta Siekmann, Rainer Meyhöfer and Martin Homme