Analgesic Activity of Hydroalcoholic Leave Extract of the Putranjiva roxburghii.

Background: this study was aimed to assess the possible analgesic activity of Putranjiva Roxburghii in albino’s Wister rats. Method: Rats were divided in 5 groups of 6 animal each, I group served as control, II group as standard (Pentazocin) while group III, IV and V were treated with leaves extract of Putranjiva Roxburghii at doses of 20,100,200 and 400 mg/kg respectively. The statistical analysis of results were carried out using and one way (ANOVA) followed by students t-test. Result and Discussion: the analgesic activity was determined based on the reaction time. The effect of the hydroalcoholic leaves extract of Putranjiva Roxburghii and references also evaluated. The hydroalcoholic leaves extract of Putranjiva Roxburghii administered orally the four different doses produced significant analgesic activity and reduced Pentazocin induced reaction time (analgesic effect) in dose dependent manner. The effect of 400 mg/kg (p.o.) having better activity than 100 and 200 mg/kg was similar to that of reference drug Pentazocin (5 mg/kg, p.o.). Conclusion: the results showed that hydroalcoholic leave extract of Putranjiva Roxburghii has a 400 mg/kg act as significant for analgesic activity. Keywords: Analgesic, Pentazocin, reaction time

Alcoholic Extract of Eclipta alba

As per WHO estimates, 80% of people around the world use medicinal plants for the cure and prevention of various diseases including cancer owing to their easy availability and cost effectiveness. Eclipta alba has long been used in Ayurveda to treat liver diseases, eye ailments, and hair related disorders. The promising medicinal value of E. alba prompted us to study the antioxidant, nontoxic, and anticancer potential of its alcoholic extract. In the current study, we evaluated the in vitro cytotoxic and antioxidant effect of the alcoholic extract of Eclipta alba (AEEA) in multiple cancer cell lines along with control. We have also evaluated its effect on different in vivo toxicity parameters. Here, we found that AEEA was found to be most active in most of the cancer cell lines but it significantly induced apoptosis in human breast cancer cell lines by disrupting mitochondrial membrane potential and DNA damage. Moreover, AEEA treatment inhibited migration in both MCF 7 and MDA-MB-231 cells in a dose dependent manner. Further, AEEA possesses robust in vitro antioxidant activity along with high total phenolic and flavonoid contents. In summary, our results indicate that Eclipta alba has enormous potential in complementary and alternative medicine for the treatment of cancer

Saraca indica

Medicinal plants are used as a complementary and alternative medicine in treatment of various diseases including cancer worldwide, because of their ease of accessibility and cost effectiveness. Multicomposed mixture of compounds present in a plant extract has synergistic activity, increases the therapeutic potential many folds, compensates toxicity, and increases bioavailability. Saraca indica (family Caesalpiniaceae) is one of the most ancient sacred plants with medicinal properties, exhibiting a number of pharmacological effects. Antioxidant, antibreast cancer activity and toxicological evaluation of Saraca indica bark extract (SIE) were carried out in the present study. The results of the study indicated that this herbal preparation has antioxidant and antibreast cancer activity. Toxicological studies suggest that SIE is safer to use and may have a potential to be used as complementary and alternative medicine for breast cancer therapy

Trimetallic Au/Pt/Ag based nanofluid for enhanced antibacterial response

The antimicrobial activity of trimetallic Au/Pt/Ag nanoparticle based nanofluids were studied and compared with that of monometallic Au and bimetallic Au/Pt nanofluid. The trimetallic nanofluid was prepared by green microwave assisted successive chemical reduction method and characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and high resolution transmission electron microscopy (HRTEM). The metallic nanofluids were tested for antibacterial properties using agar disc diffusion test method against various micro-organisms and their minimum inhibitory concentration (MIC) values were calculated. The trimetallic nanofluid exhibited efficient antibacterial activity and were found to be better agent than bimetallic and single metallic nanofluid at a very low metal concentration. These results of trimetallic nanofluids may be utilized in the field of many applications such as medical research, pharmaceutical industries and environmental sciences

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Not AvailableMungbean is affected by several diseases incited by viruses, fungi, bacteria and nematodes. In this study, coat-protein sequence was analyzed and modelled to explore properties and structure of Mungbean yellow mosaic India virus (MYMIV). Since no structural information is available for majority of pro tein sequenc es a vailable in Protein Data Bank, computational methods for protein structure prediction have been of much interest in recent years. Physico-chemical properties of the protein under study were computed. The grand average of hydropathy (GRAVY) value and instability index were found to be -0.646 and 45.75, respectively, supporting the protein to have better interaction with water. Comparative homology modelling was performed with ModWeb, ESyPred and Swiss Model. It was observed that the model generated by ModWeb was most acceptable with 90.2% of the residues in most favoured region. The model was validated using Structural Analysis and Verification Server (SAVS). Coat-protein struc ture of MYMIV obtained throug h computational modelling was found congruent with their protein structure obtained by X-ray crystallography or NMR.Not Availabl

General neuropharmacological studies of piper betel leaves on mice

The Piper betelplant is an evergreen and perennial creeper which is used in several traditional medicines to cure various diseases. The liquid extract of the plant has been used traditionally in curing inflammationand infection of the respiratory tract, cough, dyspnoea, indigestion,diphtheria, hysteria as well as general and sexual debility. The Indian traditional system ofmedicine has identified the Piper betelleaves with digestive and pancreatic lipasestimulant activities. In the present study, 14-day subacute toxicity assays were carried out.The crude extract did not produce toxic symptoms in mice in dose up to 2000mg/kg. This was also contirmed by hematological and histopathology examination.Piper betelshowed hypotensive, cardiac, and respiratory depressant effectssmooth and skeletal muscles relaxant actions, antimicrobial, fungicidal,and nematocidal activity.Piper betel leaves extract contains large number of bioactive molecule like polyphenol, alkaloids, steroids, saponin, and tannin.Alkaloid is the main constituent which is present in the extract has been detected by the Rf. values which were found from the TLC chromatography (HPTLC) has been performed.HPTLC also has been performed

Next Generation Sequencing: Potential and Application in Drug Discovery

The world has now entered into a new era of genomics because of the continued advancements in the next generation high throughput sequencing technologies, which includes sequencing by synthesis-fluorescent in situ sequencing (FISSEQ), pyrosequencing, sequencing by ligation using polony amplification, supported oligonucleotide detection (SOLiD), sequencing by hybridization along with sequencing by ligation, and nanopore technology. Great impacts of these methods can be seen for solving the genome related problems of plant and animal kingdom that will open the door of a new era of genomics. This may ultimately overcome the Sanger sequencing that ruled for 30 years. NGS is expected to advance and make the drug discovery process more rapid