Partial (13)C isotopic enrichment of nucleoside monophosphates: useful reporters for NMR structural studies

Analysis of the (13)C isotopic labeling patterns of nucleoside monophosphates (NMPs) extracted from Escherichia coli grown in a mixture of C-1 and C-2 glucose is presented. By comparing our results to previous observations on amino acids grown in similar media, we have been able to rationalize the labeling pattern based on the well-known biochemistry of nucleotide biosynthesis. Except for a few notable absences of label (C4 in purines and C3′ in ribose) and one highly enriched site (C1′ in ribose), most carbons are randomly enriched at a low level (an average of 13%). These sparsely labeled NMPs give less complex NMR spectra than their fully isotopically labeled analogs due to the elimination of most (13)C–(13)C scalar couplings. The spectral simplicity is particularly advantageous when working in ordered systems, as illustrated with guanosine diphosphate (GDP) bound to ADP ribosylation factor 1 (ARF1) aligned in a liquid crystalline medium. In this system, the absence of scalar couplings and additional long-range dipolar couplings significantly enhances signal to noise and resolution

Advanced space system concepts and their orbital support needs (1980 - 2000). Volume 1: Executive summary

The likely system concepts which might be representative of NASA and DoD space programs in the 1980-2000 time period were studied along with the programs' likely needs for major space transportation vehicles, orbital support vehicles, and technology developments which could be shared by the military and civilian space establishments in that time period. Such needs could then be used by NASA as an input in determining the nature of its long-range development plan. The approach used was to develop a list of possible space system concepts (initiatives) in parallel with a list of needs based on consideration of the likely environments and goals of the future. The two lists thus obtained represented what could be done, regardless of need; and what should be done, regardless of capability, respectively. A set of development program plans for space application concepts was then assembled, matching needs against capabilities, and the requirements of the space concepts for support vehicles, transportation, and technology were extracted. The process was pursued in parallel for likely military and civilian programs, and the common support needs thus identified

Advanced space system concepts and their orbital support needs (1980 - 2000). Volume 2: Final report

The results are presented of a study which identifies over 100 new and highly capable space systems for the 1980-2000 time period: civilian systems which could bring benefits to large numbers of average citizens in everyday life, much enhance the kinds and levels of public services, increase the economic motivation for industrial investment in space, expand scientific horizons; and, in the military area, systems which could materially alter current concepts of tactical and strategic engagements. The requirements for space transportation, orbital support, and technology for these systems are derived, and those requirements likely to be shared between NASA and the DoD in the time period identified. The high leverage technologies for the time period are identified as very large microwave antennas and optics, high energy power subsystems, high precision and high power lasers, microelectronic circuit complexes and data processors, mosaic solid state sensing devices, and long-life cryogenic refrigerators

Advanced space system concepts and their orbital support needs (1980 - 2000). Volume 3: Detailed data. Part 1: Catalog of initiatives, functional options, and future environments and goals

The following areas were discussed in relation to a study of the commonality of space vehicle applications to future national needs: (1) index of initiatives (civilian observation, communication, support), brief illustrated description of each initiative, time periods (from 1980 to 2000+) for implementation of these initiatives; (2) data bank of functional system options, presented in the form of data sheets, one for each of the major functions, with the system option for near-term, midterm, and far-term space projects applicable to each subcategory of functions to be fulfilled; (3) table relating initiatives and desired goals (public service and humanistic, materialistic, scientific and intellectual); and (4) data on size, weight and cost estimations

The stability of the O(N) invariant fixed point in three dimensions

We study the stability of the O(N) fixed point in three dimensions under perturbations of the cubic type. We address this problem in the three cases $N=2,3,4$ by using finite size scaling techniques and high precision Monte Carlo simulations. It is well know that there is a critical value $2<N_c<4$ below which the O(N) fixed point is stable and above which the cubic fixed point becomes the stable one. While we cannot exclude that $N_c<3$, as recently claimed by Kleinert and collaborators, our analysis strongly suggests that $N_c$ coincides with 3.Comment: latex file of 18 pages plus three ps figure