79 research outputs found
The Lotic Intersite Nitrogen Experiments: an example of successful ecological research collaboration
Collaboration is an essential skill for modern ecologists because it brings together diverse expertise, viewpoints, and study systems. The Lotic Intersite Nitrogen eXperiments (LINX I and II), a 17-y research endeavor involving scores of early- to late-career stream ecologists, is an example of the benefits, challenges, and approaches of successful collaborative research in ecology. The scientific success of LINX reflected tangible attributes including clear scientific goals (hypothesis-driven research), coordinated research methods, a team of cooperative scientists, excellent leadership, extensive communication, and a philosophy of respect for input from all collaborators. Intangible aspects of the collaboration included camaraderie and strong team chemistry. LINX further benefited from being part of a discipline in which collaboration is a tradition, clear data-sharing and authorship guidelines, an approach that melded field experiments and modeling, and a shared collaborative goal in the form of a universal commitment to see the project and resulting data products through to completion
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Development of a “smart” tracer for the assessment of microbiological activity and sediment-water interaction in natural waters: The resazurin-resorufin system
A “smart” tracer is a tracer that provides, directly or through measurement of its concentration or in combination with another compound, at least one “bit” more of information about the environment through which it travels than a conservative tracer. In this study we propose and present the chemical compound resazurin as a smart tracer to assess the coupling between solute transport and microbiological activity in sediment-water interfaces in freshwaters. Resazurin is a weakly fluorescent redox-sensitive dye that undergoes an irreversible reduction to strongly fluorescent resorufin under mildly reducing conditions, most commonly in the presence of living microorganisms. To investigate the suitability of resazurin as a smart tracer, we characterized the decay, sorption, reaction, and transport behavior of resazurin and resorufin in various waters and sediments using laboratory experiments. Results show that resazurin irreversibly and rapidly reacts to resorufin in colonized sediment with pseudo-first-order behavior and a rate coefficient of 1.41 h⁻¹. This reaction is 3 orders of magnitude faster than that in stream water alone, indicating the tracer is sensitive to microbiological activity and associated sediment-water interactions. The compounds are affected by significant sorption, with an approximately linear isotherm and a K[subscript d] of 6.63 mL/g for resorufin in sediment with 2.19% organic carbon. The compounds are stable over weeks in natural water, except in the presence of strong light where significant photochemical decay may occur more rapidly
Riparian Corridors: A New Conceptual Framework for Assessing Nitrogen Buffering Across Biomes
Anthropogenic activities have more than doubled the amount of reactive nitrogen circulating on Earth, creating excess nutrients across the terrestrial-aquatic gradient. These excess nutrients have caused worldwide eutrophication, fundamentally altering the functioning of freshwater and marine ecosystems. Riparian zones have been recognized to buffer diffuse nitrate pollution, reducing delivery to aquatic ecosystems, but nutrient removal is not their only function in river systems. In this paper, we propose a new conceptual framework to test the capacity of riparian corridors to retain, remove, and transfer nitrogen along the continuum from land to sea under different climatic conditions. Because longitudinal, lateral, and vertical connectivity in riparian corridors influences their functional role in landscapes, we highlight differences in these parameters across biomes. More specifically, we explore how the structure of riparian corridors shapes stream morphology (the river's spine), their multiple functions at the interface between the stream and its catchment (the skin), and their biogeochemical capacity to retain and remove nitrogen (the kidneys). We use the nitrogen cycle as an example because nitrogen pollution is one of the most pressing global environmental issues, influencing directly and indirectly virtually all ecosystems on Earth. As an initial test of the applicability of our interbiome approach, we present synthesis results of gross ammonification and net nitrification from diverse ecosystems
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Nutrient uptake in a stream affected by hydropower plants: comparison between stream channels and diversion canals
Small hydropower plants divert part of the water from wide and physically complex
stream channels with active hyporheic areas to narrow and hydraulically simple concrete canals,
and thus, might affect nutrient dynamics. We compared nutrient uptake in diversion canals and
in stream channels in the Leitzaran Stream (Basque Country, northern Spain). We hypothesized
that simple morphology in diversion canals will result in lower nutrient uptake in canals than in
stream channels.
Periphytic chlorophyll and biomass did not differ significantly between reach types. Water
was significantly deeper and faster in canals than in stream channels, but the transient storage
zone did not differ significantly between reach types. There were no significant differences
between uptake length for neither phosphate nor ammonium between reach types. Uptake
length in both stream channels and diversion canals decreased with discharge, in a pattern
similar to that previously described for pristine rivers across the world. Uptake velocity and
uptake rate for phosphate did not differ significantly between reach types, but in the case of
ammonium both retention metrics were significantly larger in the diversion canals. Results
suggest that although hydropower schemes have minor effects on nutrient retention, these
depend on the proportion of flow diverted.Keywords: nitrogen, water diversion, stream, hydromorphology, uptake, phosphorus, hydropower plantKeywords: nitrogen, water diversion, stream, hydromorphology, uptake, phosphorus, hydropower plan
Mammotome: Less Invasive than ABBI with Similar Accuracy for Early Breast Cancer Detection
We performed a prospective analysis of two consecutive biopsy cohorts investigated by the same team to compare the Mammotome system with the ABBI procedure. From April 1997 to August 2003 a series of 413 nonpalpable mammographic lesions in 387 women (median age 56 years, range 30-84 years) were stereotactically biopsied in the University Hospital of Basel, Switzerland. Until October 1999 the ABBI system was applied exclusively, it was subsequently superseded by the Mammotome device in our clinic. Main outcome measures were accuracy, technical demand, and morbidity. Sensitivity (97.3%/96.8%), negative predictive value (99.2%/98.7%), and diagnostic accuracy (99.4%/99.1%) regarding the detection of malignancy were excellent for both techniques (ABBI/Mammotome). The Mammotome procedure was faster and less invasive, thus causing significantly less morbidity. The larger specimen obtained by the ABBI procedure resulted in more detailed histology. In conclusion, recommend the Mammotome system as the method of choice for detecting nonpalpable early breast cance
Analysis of Chagas disease vectors occurrence data: the Argentinean triatomine species database
Chagas disease is a neglected tropical disease and Trypanosoma cruzi (its etiological agent) is mainly transmitted by triatomines (Hemiptera: Reduviidae). All triatomine species are considered as potential vectors; thus, their geographic distribution and habitat information should be a fundamental guide for the surveillance and control of Chagas disease. Currently, of the 137 species distributed in the Americas (Justi and Galvão 2017), 17 species are cited for Argentina: Panstrongylus geniculatus, P. guentheri, P. megistus, P. rufotuberculatus, Psammolestes coreodes, Triatoma breyeri, T. delpontei, T. eratyrusiformis, T. garciabesi, T. guasayana, T. infestans, T. limai, T. patagonica, T. platensis, T. rubrofasciata, T. rubrovaria and T. sordida. Almost 20 years have passed since the publication of the “Atlas of the Triatominae” by Carcavallo et al. (1998) and no work has been done to provide an updated complete integration and analysis of the existing information for Argentinean triatomine species. Here we provide a detailed temporal, spatial and ecological analysis of updated occurrence data for triatomines present in Argentina.Fil: Ceccarelli, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Cano, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Canale, Delmi Margarita. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; ArgentinaFil: Lobbia, Patricia Alejandra. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Stariolo, Raúl Luis. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; ArgentinaFil: Rabinovich, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentin
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Quantification of metabolically active transient storage (MATS) in two reaches with contrasting transient storage and ecosystem respiration
Water transient storage zones are hotspots for metabolic activity in streams although the contribution of different types of transient storage zones to the whole-reach metabolic activity is difficult to quantify. In this study we present a method to measure the fraction of the transient storage that is metabolically active (MATS) in two consecutive reaches with contrasting hydrological and biological characteristics. We used combined additions of resazurin (Raz) and Cl in a reach scoured to bedrock and in a reach containing a deep alluvial deposit. The MATS zones measured 0.002 m² in the bedrock reach (37% of transient storage) and 0.291 m² in the alluvial reach (100% of transient storage). The effective rate coefficient of Raz transformation in the MATS of the bedrock reach was approximately 16 times that of the alluvial reach. However, when we take into account the contribution of the MATS zone to overall metabolic activity, Raz transformation in the MATS zone was 2.2 times slower in the bedrock reach than in the alluvial reach. The difference was similar to the difference in ecosystem respiration, which was 1.8 times lower in the bedrock reach than in the alluvial reach, suggesting that the MATS zones were important contributors to ecosystem respiration. Results indicate that the quantification of MATS can improve our understanding of the role that transient storage zones play on stream metabolic processes and demonstrate the utility of Raz as a “smart” tracer that provides new information on metabolic activity at a whole-reach and at smaller scale.This is the publisher’s final pdf. The published article is copyrighted by the American Geophysical Union and can be found at: http://www.agu.org/journals/jgr/
Draft Genome Sequence of the Polyextremophilic Halorubrum sp. Strain AJ67, Isolated from Hyperarsenic Lakes in the Argentinian Puna
Halorubrum sp. AJ67, an extreme halophilic, UV resistant archae that was isolated from Laguna Antofalla in the Argentinean Puna. The draft genome sequence suggests potent enzyme candidates that are essential to survive in multiple environmental extreme conditions, as high UV radiation, elevated salinity and the presence of critical arsenic concentration.Fil: Burguener, Germán Federico. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo. Plataforma de Bioinformática Argentina; ArgentinaFil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; ArgentinaFil: Revale, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Fernández Do Porto, Darío Augusto. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo. Plataforma de Bioinformática Argentina; ArgentinaFil: Rascovan, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo. Plataforma de Bioinformática Argentina; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Marti, Marcelo Adrian. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo. Plataforma de Bioinformática Argentina; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Turjanski, Adrian. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo. Plataforma de Bioinformática Argentina; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentin
Factors affecting ammonium uptake in streams - an inter-biome perspective
The Lotic Intersite Nitrogen experiment (LINX) was a coordinated study of the relationships between North American biomes and factors governing ammonium uptake in streams. Our objective was to relate inter-biome variability of ammonium uptake to physical, chemical and biological processes. 2. Data were collected from 11 streams ranging from arctic to tropical and from desert to rainforest. Measurements at each site included physical, hydraulic and chemical characteristics, biological parameters, whole-stream metabolism and ammonium uptake. Ammonium uptake was measured by injection of \u275~-ammonium and downstream measurements of 15N-ammonium concentration. 3. We found no general, statistically significant relationships that explained the variability in ammonium uptake among sites. However, this approach does not account for the multiple mechanisms of ammonium uptake in streams. When we estimated biological demand for inorganic nitrogen based on our measurements of in-stream metabolism, we found good correspondence between calculated nitrogen demand and measured assimilative nitrogen uptake. 4. Nitrogen uptake varied little among sites, reflecting metabolic compensation in streams in a variety of distinctly different biomes (autotrophic production is high where allochthonous inputs are relatively low and vice versa). 5. Both autotrophic and heterotrophic metabolism require nitrogen and these biotic processes dominate inorganic nitrogen retention in streams. Factors that affect the relative balance of autotrophic and heterotrophic metabolism indirectly control inorganic nitrogen uptake
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