Bioprospecting and characterization of temperature tolerant microalgae from Bonaire

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Microsatellite Markers Isolated From Cabomba Aquatica Sl (cabombaceae) From An Enriched Genomic Library

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Premise of the study: Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. Methods and Results: Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and -1.0 to -0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and -1.0 to 0.4643 for the Virua population. Conclusions: The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.311Post-Graduate program in Plant Biology of the Instituto de Biologia (UNICAMP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES [PNADB 457/2010

On Energy Balance and Production Costs in Tubular and Flat Panel Photobioreactors

Reducing mixing in both flat panel and tubular photobioreactors can result in a positive net energy balance with state-of-the-art technology and Dutch weather conditions. In the tubular photobioreactor, the net energy balance becomes positive at velocities < 0.3 ms-1, at which point the biomass production cost is 3.2 €/kg dry weight. In flat panel reactors, this point is at an air supply rate < 0.25 vol vol-1 min-1, at which the biomass production cost is 2.39 €/kg dry weight. To achieve these values in flat panel reactors, cheap low pressure blowers must be used, which limits the panel height to a maximum of 0.5 m, and in tubular reactors the tubes must be hydraulically smooth. For tubular reactors, it is important to prevent the formation of wall growth in order to keep the tubes hydraulically smooth. This paper shows how current production costs and energy requirement could be decreased.Reducing mixing in both flat panel and tubular photobioreactors can result in a positive net energy balance with state-of-the-art technology and Dutch weather conditions. In the tubular photobioreactor, the net energy balance becomes positive at velocities < 0.3 ms-1, at which point the biomass production cost is 3.2 €/kg dry weight. In flat panel reactors, this point is at an air supply rate < 0.25 vol vol-1 min-1, at which the biomass production cost is 2.39 €/kg dry weight. To achieve these values in flat panel reactors, cheap low pressure blowers must be used, which limits the panel height to a maximum of 0.5 m, and in tubular reactors the tubes must be hydraulically smooth. For tubular reactors, it is important to prevent the formation of wall growth in order to keep the tubes hydraulically smooth. This paper shows how current production costs and energy requirement could be decreased

Microalgae production cost in aquaculture hatcheries

Microalgae are a crucial part in many aquaculture feed applications processes, mainly in hatcheries. Many aquaculture hatcheries maintain a small scale microalgae production facility in-house for the production of live feed. Microalgae are usually grown in non-automated bubble-column systems at unknown production costs. Other reactor systems or scenarios utilizing artificial light or sunlight and at different scales could result in a more cost efficient production processes. To determine the cost-price and cost-distribution of microalgae production facilities in Dutch aquaculture industry and identify the most efficient cost reducing strategies a techno-economic analysis for small scale microalgae production facilities (25-1500 m2) was developed. Commercially available reactors commonly used in aquaculture were compared; tubular photobioreactors (TPBR) and bubble-columns (BC) in two placement possibilities; using artificial light in an indoor facility (AL) and utilizing sunlight in a greenhouse (GH) under Dutch climate conditions. Data from commercial microalgae facilities in the Netherlands are used to model reference scenarios describing the cost price of microalgae production with state of the art technology in aquaculture for a biomass production capacity of 125 kg year−1. The reference cost price for algae biomass (on the basis of dry matter) is calculated at €290,- kg−1 and € 329 kg−1 for tubular reactors under artificial light and a greenhouse, respectively and €587,- kg−1 and €573 kg−1 for bubble-columns under artificial light and a greenhouse, respectively. The addition of more artificial light will significantly reduce production costs (by 33%) in all small-scale systems modelled. Biomass yield on light (Yx,ph) showed the largest effect on cost price when not considering a different scale of the production process. Process parameters like temperature control should be aimed at optimizing Yx,ph rather than other forms of cost reduction. The scale of a microalgae production facility has a very large impact on the cost price. With state of the art technologies a cost price reduction of 92% could be achieved by changing the scale from 25m2 to 1500m2, resulting in a cost price of €43,- kg−1, producing 3992 kg year−1 for tubular reactors in a greenhouse. The presented techno-economic model gives valuable insights in the cost price distribution of microalgae production in aquaculture. This allows to focus research efforts towards the most promising cost reduction methods and to optimize existing production facilities in aquaculture companies to achieve economically sustainable microalgae production for live feed in hatcheries.publishedVersionPaid Open Acces

Prospects for viruses infecting eukaryotic microalgae in biotechnology

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Optimization of Rhodomonas sp. under continuous cultivation for industrial applications in aquaculture

The microalgae species Rhodomonas sp. is commonly used in aquaculture for its high nutritional value due to the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) content. Understanding the effect of cultivation parameters on biomass production rate and composition is presently limited, however essential in further commercialization of this strain. Under nutrient replete conditions, light intensity and temperature are the main factors determining biomass growth and composition. Therefore, the combined effect of light and temperature on the biomass production rate and biomass composition of Rhodomonas sp. was studied using a statistical Design of Experiment approach. Rhodomonas sp. was cultivated under continuous (turbidostat) conditions in lab-scale reactor systems (1.8 l) under different temperature (15–20–25–30 °C) and light conditions (60–195–330–465–600 μmol m−2 s−1). Stable biomass production was observed under all conditions except experiments performed at 30 °C, which led to cell death. Under optimized growth conditions, high growth rates (>1.0d−1) and high biomass production rates, up to 1.5 g l−1 d−1, were obtained in this study. The biomass production rate reported here is >10-fold higher than values reported in literature on Rhodomonas sp. The optimal temperature for maximal growth was found at T = 22–24 °C under all light conditions. The maximum biomass yield on light (Yx,ph – 0.87 g mol−1) was found at light levels between 110 and 220 μmol m−2 s−1. The fatty acid profile was only significantly influenced by temperature, with higher EPA and DHA contents at lower temperatures (15 °C). A total fatty acid (TFA) content of 8–10% of the total dry-weight was found for all tested conditions. The EPA content fluctuated between 9 and 16% of TFA and DHA content between 6 and 9% of TFA, only affected by temperature. A maximum EPA + DHA production rate of 114 mg l‐−1 d−1 was obtained at 20 °C and high light (600 μmol m−2 s−1) conditions.publishedVersionPaid Open Acces

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

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Process optimization of fucoxanthin production with Tisochrysis lutea

To optimize fucoxanthin production in Tisochrysis lutea, the effect of different process parameters on fucoxanthin productivity (Pfx) were evaluated using batch and continuous experiments. In batch, the highest Pfx was found at 30 °C and 300 μmol m−2 s−1, allowing to design continuous experiments to optimize the dilution rate. The highest ever reported Pfx (9.43–9.81 mg L−1 d−1) was achieved at dilution rates of 0.53 and 0.80 d−1. Irradiance was varied (50–500 μmol m−2 s−1) to result in a range of absorbed light between 2.23 and 25.80 mol m−2 d−1 at a fixed dilution rate (0.53 d−1). These experiments validated the hypothesis that light absorbed can be used to predict fucoxanthin content, resulting in 2.23 mol m−2 d−1 triggering the highest fucoxanthin content (16.39 mg/g). The highest Pfx was found with 18.38 mol m−2 d−1. These results can be used to achieve high Pfx or fucoxanthin content during cultivation of Tisochrysis lutea.publishedVersionPaid Open Acces

Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea

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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

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