Gorlin syndrome associated with small bowel carcinoma and mesenchymal proliferation of the gastrointestinal tract: case report and review of literature

<p>Abstract</p> <p>Background and Case Presentation</p> <p>A patient with nevoid basal cell carcinoma syndrome (Gorlin syndrome) presented with two unusual clinical features, i.e. adenocarcinoma of the small bowel and extensive mesenchymal proliferation of the lower gastrointestinal tract.</p> <p>Conclusions</p> <p>We discuss the possibility that these two features are pathogenetically linked to the formerly undescribed patient's <it>PTCH </it>germ line mutation.</p

Alterations in RAPD profiles of proliferating shootlets of sugarcane in response to thidiazuron

207-210Proliferating shootlets of sugarcane varieties CoH 92 and Co 7717, raised from apical meristems, were cultured in vitro for 24 d in MS medium with different concentration of TDZ. DNA from leaves of these shootlets was extracted and amplified using 10 random decamer primers. A total of 50 RAPD products in var. CoH 92 and 43 in var. Co 7717 were formed. TDZ caused considerable differences in amplification products of both varieties. Several RAPD products, which were absent in control plantlets, amplified in TDZ treatments, such as 950 bp (CoH 92 and Co 7717) against primer 5´-AATCGGGCTG-3´ (10A04); 1200 bp (Co 7717) against primer 5´-AGCCAGCGAA-3´ (50A16); 1150 and 1000 bp (CoH 92 and Co 7717) and 900 bp (Co 7717) against primer 5´-CTGCTGGGAC-3´ (80B10); 950 bp (CoH-92) and 500 bp (Co 7717) against primer 5´-GTAGACCCGT-3´ (90B11); and 500 bp (CoH 92) against primer 5´-CCGCATCTAC-3´ (100C04). On the other hand, certain RAPD products, such as 220 bp (CoH 92) against primer 5´-TCGGCGATAG-3´ (40A10); 950 bp (Co 7717) against primer 50A16; 550, 360 and 320 bp (CoH 92) against primer 5´-GGACTGGAGT-3´ (60B04); and 1050 bp (CoH 92) against primer 80B10, amplified in the control plantlets disappeared due to TDZ treatments. Such altered RAPD profile in response to TDZ is an entirely new and hitherto unsuspected observation, which suggests a possible genetic change or perhaps to a synthesis of protein or non-protein regulators that affect the process of DNA synthesis/amplification

Permanent genetic resources added to Molecular Ecology Resources Database 1 december 201231 january 2013

This article documents the addition of 268 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Alburnoidesbipunctatus, Chamaeropshumilis, Chlidoniashybrida, Cyperuspapyrus, Fusariumgraminearum, Loxigillabarbadensis, Macrobrachiumrosenbergii, Odontesthesbonariensis, Pelteobagrusvachelli, Posidoniaoceanica, Potamotrygonmotoro, Rhamdia quelen, Sarotherodonmelanotheron heudelotii, Sibiraeaangustata, Takifugurubripes, Tarentolamauritanica, Trimmatostroma sp. and Wallagoattu. These loci were cross-tested on the following species: Alburnoides fasciatus, Alburnoides kubanicus, Alburnoides maculatus, Alburnoides ohridanus, Alburnoides prespensis, Alburnoides rossicus, Alburnoides strymonicus, Alburnoides thessalicus, Alburnoides tzanevi, Carassius carassius, Fusarium asiaticum, Leucaspius delineatus, Loxigilla noctis dominica, Pelecus cultratus, Phoenix canariensis, Potamotrygon falkneri, Trachycarpus fortune and Vimba vimba