20 research outputs found
Heterozygosity at neutral and immune loci is not associated with neonatal mortality due to microbial infection in Antarctic fur seals.
Litzke V, Ottensmann M, Forcada J, Heitzmann L, Hoffman J. Heterozygosity at neutral and immune loci is not associated with neonatal mortality due to microbial infection in Antarctic fur seals. Ecology and evolution. 2019;9(14):7985-7996.Numerous studies have reported correlations between the heterozygosity of genetic markers and fitness. These heterozygosity-fitness correlations (HFCs) play a central role in evolutionary and conservation biology, yet their mechanistic basis remains open to debate. For example, fitness associations have been widely reported at both neutral and functional loci, yet few studies have directly compared the two, making it difficult to gauge the relative contributions of genome-wide inbreeding and specific functional genes to fitness. Here, we compared the effects of neutral and immune gene heterozygosity on death from bacterial infection in Antarctic fur seal (Arctocephalus gazella) pups. We specifically developed a panel of 13 microsatellites from expressed immune genes and genotyped these together with 48 neutral loci in 234 individuals, comprising 39 pups that were classified at necropsy as having most likely died of bacterial infection together with a five times larger matched sample of healthy surviving pups. Identity disequilibrium quantified from the neutral markers was positive and significant, indicative of variance in inbreeding within the study population. However, multilocus heterozygosity did not differ significantly between healthy and infected pups at either class of marker, and little evidence was found for fitness associations at individual loci. These results support a previous study of Antarctic fur seals that found no effects of heterozygosity at nine neutral microsatellites on neonatal survival and thereby help to refine our understanding of how HFCs vary across the life cycle. Given that nonsignificant HFCs are underreported in the literature, we also hope that our study will contribute toward a more balanced understanding of the wider importance of this phenomenon
Comparing the Affective Outcomes of CLIL Modules and Streams on Secondary School Students
Ohlberger S, Litzke V, Wegner C. Comparing the Affective Outcomes of CLIL Modules and Streams on Secondary School Students. RISTAL. Research in Subject-matter Teaching and Learning. 2019;(2):61-84
Educating recently immigrated students: Exploring the suitability of scientific Modules in secondary education
Schmiedebach M, Litzke V, Wegner C. Educating recently immigrated students: Exploring the suitability of scientific Modules in secondary education. Progress In Science Education (PriSE). 2020;2(1):28-35
Predispositions define a pro-environmental attitude
Schmiedebach M, Litzke V, Laumen S, Wegner C. Predispositions define a pro-environmental attitude. Journal of STEAM Education. 2022;5(1):1-14
Supplementary Table S2
96 microsatellite loci selected for testing, comprising twenty that appeared polymorphic in silico as described by Hoffman and Nichols (2011) plus a further 76 loci that carried at least six repeat units
Supplementary Table S4
Summary of the 48 neutral loci and the two previously published immune loci (Hoffman and Nichols, 2011) genotyped in this study, including their polymorphism characteristics in 234 Antarctic fur seal individuals
Supplementary Table S1
2362 microsatellite loci identified from the Antarctic fur seal transcriptome assembly that consist of a minimum of five short tandem repeats with perfect di-, tri- and tetranucleotide motifs