Pre-Merger Localization of Gravitational-Wave Standard Sirens With LISA I: Harmonic Mode Decomposition

The continuous improvement in localization errors (sky position and distance) in real time as LISA observes the gradual inspiral of a supermassive black hole (SMBH) binary can be of great help in identifying any prompt electromagnetic counterpart associated with the merger. We develop a new method, based on a Fourier decomposition of the time-dependent, LISA-modulated gravitational-wave signal, to study this intricate problem. The method is faster than standard Monte Carlo simulations by orders of magnitude. By surveying the parameter space of potential LISA sources, we find that counterparts to SMBH binary mergers with total mass M~10^5-10^7 M_Sun and redshifts z<~3 can be localized to within the field of view of astronomical instruments (~deg^2) typically hours to weeks prior to coalescence. This will allow targeted searches for variable electromagnetic counterparts as the merger proceeds, as well as monitoring of the most energetic coalescence phase. A rich set of astrophysical and cosmological applications would emerge from the identification of electromagnetic counterparts to these gravitational-wave standard sirens.Comment: 29 pages, 12 figures, version accepted by Phys Rev

The investigation of additive manufacturing and moldable materials to produce railway ballast grain analogs

The size and shape of individual grains, play an important role in the mechanical behavior of granular materials such as the strength and stability of railway ballast. The aim of this research is to study materials from which uniform, reproducible grains with irregular convex geometry can be created by molding and additive manufacturing technologies in order to create reproducible artificial assemblies that can be used in experiments. Packings with determined grain shape results more controlled investigations contrarily to using natural grains with random geometry. Specimens were made from railway ballast materials, materials used in the construction industry, additively manufactured and molded polymers, and certain low-strength materials. Uniaxial compression and bending tests were conducted on these specimens. The mechanical properties of typical railway ballast materials (basalt and andesite) were compared with the properties of artificially produced materials. The results show that for grain reproduction the molding technology is recommended with the use of polyester-crushed stone composite and ceramic powder. Furthermore, the additive manufacturing was recommended with PolyJet or Multi Jet Fusion technology as they have the feasibility to produce grains with similar material properties to the properties of basalt and andesite

Agmatine and Agmatine Analogs in the Treatment of Epilepsy, Seizure, and Electroconvulsive Disorders

Pharmaceutical preparations containing of agmatine, congeners, analogs or derivatives thereof for use in preventing or treating epilepsy, seizures and other electroconvulsive disorders are provided. Embodiments include administering an effective amount of agmatine, an agmatine analog or a pharmaceutically acceptable salt thereof to a human subject in need of treatment or prevention of epilepsy, seizure or other electroconvulsive disorder to treat, reduce, or prevent the disorder in the subject

Compounds of Use in the Treatment of Epilepsy, Seizure, and Electroconvulsive Disorders

The present invention provides pharmaceutical preparations and the uses thereof for preventing and/or treating seizures and other electroconvulsive disorders by administering a pharmaceutically effective amount of a therapeutic compound . . . To view the rest of this abstract, please download this patent

Repeated Bursts from Relativistic Scattering of Compact Objects in Galactic Nuclei

Galactic nuclei are densely populated by stellar mass compact objects such as black holes and neutron stars. Bound, highly eccentric binaries form as a result of gravitational wave (GW) losses during close flybys between these objects. We study the evolution of these systems using 2.5 and 3.5 order post-Newtonian equations of motion. The GW signal consists of many thousand repeated bursts (RB) for minutes to days (depending on the impact parameter and masses), followed by a powerful GW chirp and an eccentric merger. We show that a significant signal to noise ratio (SNR) accumulates already in the RB phase, corresponding to a detection limit around 200-300 Mpc and 300--600 Mpc for Advanced LIGO for an average orientation BH/NS or BH/BH binary, respectively. The theoretical errors introduced by the inaccuracy of the PN templates are typically much less severe for the RB phase than in the following eccentric merger. The GW signal in the RB phase is broadband; we show that encounters involving intermediate mass black holes are detectable in multiple frequency bands coincidentally using LIGO and LISA.Comment: 16 pages, 10 figures, version accepted for publication in Physical Review

Effect of temperature, pH and calcium phosphate concentration on the properties of reassembled casein micelles

Reassembled casein micelles (RCMs) can be made by reassembling sodium caseinate with calcium and phosphate as well as other ions under controlled conditions into casein micelle-like structures. During the reassembly, the changes in processing parameters lead to differences in the properties of RCMs such as size, composition and structure. Understanding the effect of processing parameters on RCM properties is essential for their potential application in food products. However, the effect of process parameters has not been studied systematically. Therefore, the objective of this study was to evaluate the effect of temperature, pH and calcium phosphate concentration on the properties of reassembled casein micelles. The effect of pH on the size and structure of RCMs both during and after their formation was studied by dynamic light scattering and small-angle X-ray scattering. We found that pH affects both the size and internal structure of RCMs. We could also modulate the size and composition of RCMs by changing the temperature and calcium phosphate concentration. The insights of this study not only can be used to modulate the composition and structure of RCMs, but also help us to understand how processing parameters will influence the assembly of RCMs from novel sources, such as recombinant caseins

Effect of temperature, pH and calcium phosphate concentration on the properties of reassembled casein micelles

Reassembled casein micelles (RCMs) can be made by reassembling sodium caseinate with calcium and phosphate as well as other ions under controlled conditions into casein micelle-like structures. During the reassembly, the changes in processing parameters lead to differences in the properties of RCMs such as size, composition and structure. Understanding the effect of processing parameters on RCM properties is essential for their potential application in food products. However, the effect of process parameters has not been studied systematically. Therefore, the objective of this study was to evaluate the effect of temperature, pH and calcium phosphate concentration on the properties of reassembled casein micelles. The effect of pH on the size and structure of RCMs both during and after their formation was studied by dynamic light scattering and small-angle X-ray scattering. We found that pH affects both the size and internal structure of RCMs. We could also modulate the size and composition of RCMs by changing the temperature and calcium phosphate concentration. The insights of this study not only can be used to modulate the composition and structure of RCMs, but also help us to understand how processing parameters will influence the assembly of RCMs from novel sources, such as recombinant caseins

Identification of epidermal Pdx1 expression discloses different roles of Notch1 and Notch2 in murine KrasG12D-induced skin carcinogenesis in vivo

Background The Ras and Notch signaling pathways are frequently activated during development to control many diverse cellular processes and are often dysregulated during tumorigenesis. To study the role of Notch and oncogenic Kras signaling in a progenitor cell population, Pdx1-Cre mice were utilized to generate conditional oncogenic KrasG12D mice with ablation of Notch1 and/or Notch2. Methodology/Principal Findings Surprisingly, mice with activated KrasG12D and Notch1 but not Notch2 ablation developed skin papillomas progressing to squamous cell carcinoma providing evidence for Pdx1 expression in the skin. Immunostaining and lineage tracing experiments indicate that PDX1 is present predominantly in the suprabasal layers of the epidermis and rarely in the basal layer. Further analysis of keratinocytes in vitro revealed differentiation-dependent expression of PDX1 in terminally differentiated keratinocytes. PDX1 expression was also increased during wound healing. Further analysis revealed that loss of Notch1 but not Notch2 is critical for skin tumor development. Reasons for this include distinct Notch expression with Notch1 in all layers and Notch2 in the suprabasal layer as well as distinctive p21 and β-catenin signaling inhibition capabilities. Conclusions/Significance Our results provide strong evidence for epidermal expression of Pdx1 as of yet not identified function. In addition, this finding may be relevant for research using Pdx1-Cre transgenic strains. Additionally, our study confirms distinctive expression and functions of Notch1 and Notch2 in the skin supporting the importance of careful dissection of the contribution of individual Notch receptors

Adipocyte-specific protein tyrosine phosphatase 1B deletion increases lipogenesis, adipocyte cell size and is a minor regulator of glucose homeostasis

Peer reviewedPublisher PD

Protein Tyrosine Phosphatase PTP1B is Involved in Hippocampal Synapse Formation and Learning

ER-bound PTP1B is expressed in hippocampal neurons, and accumulates among neurite contacts. PTP1B dephosphorylates ß-catenin in N-cadherin complexes ensuring cell-cell adhesion. Here we show that endogenous PTP1B, as well as expressed GFP-PTP1B, are present in dendritic spines of hippocampal neurons in culture. GFP-PTP1B overexpression does not affect filopodial density or length. In contrast, impairment of PTP1B function or genetic PTP1B-deficiency leads to increased filopodia-like dendritic spines and a reduction in mushroom-like spines, while spine density is unaffected. These morphological alterations are accompanied by a disorganization of pre- and post-synapses, as judged by decreased clustering of synapsin-1 and PSD-95, and suggest a dynamic synaptic phenotype. Notably, levels of ß-catenin-Tyr-654 phosphorylation increased ∼5-fold in the hippocampus of adult PTP1B−/− (KO) mice compared to wild type (WT) mice and this was accompanied by a reduction in the amount of ß-catenin associated with N-cadherin. To determine whether PTP1B-deficiency alters learning and memory, we generated mice lacking PTP1B in the hippocampus and cortex (PTP1Bfl/fl–Emx1-Cre). PTP1Bfl/fl–Emx1-Cre mice displayed improved performance in the Barnes maze (decreased time to find and enter target hole), utilized a more efficient strategy (cued), and had better recall compared to WT controls. Our results implicate PTP1B in structural plasticity within the hippocampus, likely through modulation of N-cadherin function by ensuring dephosphorylation of ß-catenin on Tyr-654. Disruption of hippocampal PTP1B function or expression leads to elongation of dendritic filopodia and improved learning and memory, demonstrating an exciting novel role for this phosphatase