Genetic variation and host-parasite specificity of Striga resistance and tolerance in rice: the need for predictive breeding

The parasitic weeds Striga asiatica and Striga hermonthica cause devastating yield losses to upland rice in Africa. Little is known about genetic variation in host resistance and tolerance across rice genotypes, in relation to virulence differences across Striga species and ecotypes. Diverse rice genotypes were phenotyped for the above traits in S. asiatica- (Tanzania) and S. hermonthica-infested fields (Kenya and Uganda) and under controlled conditions. New rice genotypes with either ecotype-specific or broad-spectrum resistance were identified. Resistance identified in the field was confirmed under controlled conditions, providing evidence that resistance was largely genetically determined. Striga-resistant genotypes contributed to yield security under Striga-infested conditions, although grain yield was also determined by the genotype-specific yield potential and tolerance. Tolerance, the physiological mechanism mitigating Striga effects on host growth and physiology, was unrelated to resistance, implying that any combination of high, medium or low levels of these traits can be found across rice genotypes. Striga virulence varies across species and ecotypes. The extent of Striga-induced host damage results from the interaction between parasite virulence and genetically determined levels of host-plant resistance and tolerance. These novel findings support the need for predictive breeding strategies based on knowledge of host resistance and parasite virulence

Mapping the drivers of parasitic weed abundance at a national scale : a new approach applied to Striga asiatica in the mid‐west of Madagascar

The parasitic weed genus Striga causes huge losses to crop production in sub‐Saharan Africa, estimated to be in excess of $7 billion per year. There is a paucity of reliable distribution data for Striga ; however, such data are urgently needed to understand current drivers, better target control efforts, as well as to predict future risks. To address this, we developed a methodology to enable rapid, large‐scale monitoring of Striga populations. We used this approach to uncover the factors that currently drive the abundance and distribution of Striga asiatica in Madagascar. Two long‐distance transects were established across the middle‐west region of Madagascar in which S. asiatica abundance in fields adjacent to the road was estimated. Management, crop structure and soil data were also collected. Analysis of the data suggests that crop variety, companion crop and previous crop were correlated with Striga density. A positive relationship between within‐field Striga density and the density of the nearest neighbouring fields indicates that spatial configuration and connectivity of suitable habitats is also important in determining Striga spread. Our results demonstrate that we are able to capture distribution and management data for Striga density at a landscape scale and use this to understand the ecological and agronomic drivers of abundance. The importance of crop varieties and cropping patterns is significant, as these are key socio‐economic elements of Malagasy cropping practices. Therefore, they have the potential to be promoted as readily available control options, rather than novel technologies requiring introduction

Do NERICA rice cultivars express resistance to Striga hermonthica (Del.) Benth. and Striga asiatica (L.) Kuntze under field conditions?

The parasitic weeds Striga asiatica and Striga hermonthica cause high yield losses in rain-fed upland rice in Africa. Two resistance classes (pre- and post-attachment) and several resistant genotypes have been identified among NERICA (New Rice for Africa) cultivars under laboratory conditions (in vitro) previously. However, little is known about expression of this resistance under field conditions. Here we investigated (1) whether resistance exhibited under controlled conditions would express under representative Striga-infested field conditions, and (2) whether NERICA cultivars would achieve relatively good grain yields under Striga-infested conditions. Twenty-five rice cultivars, including all 18 upland NERICA cultivars, were screened in S. asiatica-infested (in Tanzania) and S. hermonthica-infested (in Kenya) fields during two seasons. Additionally, a selection of cultivars was tested in vitro, in mini-rhizotron systems. For the first time, resistance observed under controlled conditions was confirmed in the field for NERICA-2, -5, -10 and -17 (against S. asiatica) and NERICA-1 to -5, -10, -12, -13 and -17 (against S. hermonthica). Despite high Striga-infestation levels, yields of around 1.8 t ha−1 were obtained with NERICA-1, -9 and -10 (in the S. asiatica-infested field) and around 1.4 t ha−1 with NERICA-3, -4, -8, -12 and -13 (in the S. hermonthica-infested field). In addition, potential levels of tolerance were identified in vitro, in NERICA-1, -17 and -9 (S. asiatica) and in NERICA-1, -17 and -10 (S. hermonthica). These findings are highly relevant to rice agronomists and breeders and molecular geneticists working on Striga resistance. In addition, cultivars combining broad-spectrum resistance with good grain yields in Striga-infested fields can be recommended to rice farmers in Striga-prone areas

Testing the importance of a common ectomycorrhizal network for dipterocarp seedling growth and survival in tropical forests of Borneo

Background: Connections between mature trees and seedlings via ectomycorrhizal (EcM) hyphal networks existing in dipterocarp-dominated tropical rain forests of South-east Asia could have strong implications for seedling growth and survival and the maintenance of high diversity in such forests. Aim: To test whether EcM hyphal network connections are important for the growth and survival of dipterocarp seedlings. Methods: We conducted four independent experiments that prevented contact of experimental seedlings with an EcM network by using a series of fine meshes and/or plastic barriers. We measured the growth and survival (and foliar δ13C in one experiment) of seedlings of six dipterocarp species over intervals ranging from 11 to 29 months. Results: Seedling growth (diameter, height or leaf number) was unaffected by exclusion from the EcM network in three experiments and there were no differences in foliar δ13C values in the fourth. Seedling survival was reduced following exclusion from the EcM network in one experiment. Our results give little support to the hypothesis that dipterocarp seedlings growing in the shaded forest understorey benefit from being connected, through a common EcM network, to surrounding trees. Conclusions: We suggest that our negative results, in contrast to studies conducted in low diversity boreo-temperate or tropical forests, are due to these high diversity forests lacking host species-specific EcM fungi and therefore providing little opportunity for adaptive support of seedlings via hyphal networks

Optimizing chemically induced resistance in tomato against <i>Botrytis cinerea</i>

Resistance-inducing chemicals can offer broad-spectrum disease protection in crops, but can also affect plant growth and interactions with plant-beneficial microbes. We have evaluated different application methods of β-aminobutyric acid (BABA) and jasmonic acid (JA) for long-lasting induced resistance in tomato against Botrytis cinerea. In addition, we have studied nontarget effects on plant growth and root colonization by arbuscular mycorrhizal fungi (AMF). Germinating seeds for 1 week in BABA- or JA-containing solutions promoted seed germination efficiency, did not affect plant growth, and induced resistance in 4-week-old plants. When formulating BABA and JA in carboxymethyl cellulose seed coating, only BABA was able to induce resistance in 4-week-old plants. Root treatment of 1-week-old seedlings with BABA or JA also induced resistance in 4-week-old plants. However, this seedling treatment repressed plant growth at higher concentrations of the chemicals, which was particularly pronounced in hydroponically grown plants after BABA treatment. Both seed coating with BABA, and seedling treatments with BABA or JA, did not affect AMF root colonization in soil-grown tomato. Our study has identified commercially feasible application methods of BABA and JA, which induce durable disease resistance in tomato without concurrent impacts on plant growth or colonization by plant-beneficial AMF. </jats:p