Prosjekt Nytt Nasjonalmuseum: Negotiating a Norwegian Identity

There is a vast amount of attention in Norway going into cultural institutions that is supposed define the image of the capital and Norwegian society. The focus on ‘building culture’ has nevertheless left a lacuna of what or where the Norwegian identity is in relation to this process. Former definitions of the state and the general consensus of what it means to be Norwegian have in many ways changed over the past three decades. I am therefore interested in crafting a response to the fleeting conditions of this Norwegian identity at the start of the century, by considering the way it is represented through the building of a museum. As a case study, the new National Museum of Arts, Architecture and Design in Oslo, or Nasjonalmuseet as it is better known, can in many ways be described as an interface that bridges the ‘new’ ideas with the ‘old’, and thereby revealing how the formulation of what the Norwegian identity might be is under negotiation. My discussion exists amidst a larger discourse of the role cultural institutions and urban development play in the formulation of a Norwegian identity in the twenty-first century. In addition to arrive at predictions about the new museum as such, I hope to respond to how this is reflective of a broader framework of the cultural, economic and political situations in Norway

Characterization and functionality of proliferative human sertoli cells

It has long been thought that mammalian Sertoli cells are terminally differentiated and nondividing postpuberty. For most previous in vitro studies immature rodent testes have been the source of Sertoli cells and these have shown little proliferative ability when cultured. We have isolated and characterized Sertoli cells from human cadaveric testes from seven donors ranging from 12 to 36 years of age. The cells proliferated readily in vitro under the optimized conditions used with a doubling time of approximately 4 days. Nuclear 5-ethynyl-2´-deoxyuridine (EdU) incorporation confirmed that dividing cells represented the majority of the population. Classical Sertoli cell ultrastructural features, lipid droplet accumulation, and immunoexpression of GATA-4, Sox9, and the FSH receptor (FSHr) were observed by electron and fluorescence microscopy, respectively. Flow cytometry revealed the expression of GATA-4 and Sox9 by more than 99% of the cells, and abundant expression of a number of markers indicative of multipotent mesenchymal cells. Low detection of endogenous alkaline phosphatase activity after passaging showed that few peritubular myoid cells were present. GATA-4 and SOX9 expression were confirmed by reverse transcription polymerase chain reaction (RT-PCR), along with expression of stem cell factor (SCF), glial cell line-derived neurotrophic factor (GDNF), and bone morphogenic protein 4 (BMP4). Tight junctions were formed by Sertoli cells plated on transwell inserts coated with fibronectin as revealed by increased transepithelial electrical resistance (TER) and polarized secretion of the immunoregulatory protein, galectin-1. These primary Sertoli cell populations could be expanded dramatically in vitro and could be cryopreserved. The results show that functional human Sertoli cells can be propagated in vitro from testicular cells isolated from adult testis. The proliferative human Sertoli cells should have important applications in studying infertility, reproductive toxicology, testicular cancer, and spermatogenesis, and due to their unique biological properties potentially could be useful in cell therapy.Fil: Chui, Kitty. MandalMed Inc. ; Estados UnidosFil: Trivedi, Alpa. MandalMed Inc.; Estados Unidos. University of California; Estados UnidosFil: Cheng, C. Yan. Lonza Walkersville; Estados UnidosFil: Cherbavaz, Diana B.. MandalMed Inc.; Estados UnidosFil: Dazin, Paul F.. MandalMed; Estados UnidosFil: Huynh, Ai Lam Thu. MandalMed Inc.; Estados UnidosFil: Mitchel, James B.. Lonza Walkersville; Estados UnidosFil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Noble Haeusslein, Linda J.. University of California; Estados UnidosFil: John, Constance M.. MandalMed Inc.; Estados Unido

Catalyst-free selective-area metalorganic chemical vapour deposition of InGaAs/InGaP core-shell nanowire arrays

III-V semiconductor nanowires (NWs) offer promising solutions for on-chip light sources in integrated photonics with small device footprint and ultra-low power consumption. Growing NWs by catalyst-free selective-area epitaxy (SAE) offers many advantages, such as the compatibility with silicon manufacturing and the capability to control NW diameter and position precisely. An array of NWs can be exploited as either a 1D or 2D photonic crystal, while every single NW operates as a vertical optical cavity, offering the flexibility for photodetector and laser designs. In this work, we demonstrate catalyst-free selective area metalorganic chemical vapour deposition (MOCVD) of InGaAs/InGaP core-shell NW arrays on patterned n-GaAs (111)B substrates, with an aim to realize high-performance avalanche photodiodes toward single photon sensing at near-infrared (NIR) wavelengths and bottom-up photonic crystal cavities. By carefully optimizing the growth parameters, the core-shell nanowires show a high uniformity in diameter (~180 nm) as well as in height (~1.6 μm), where a vertical growth yield of ~100% was obtained on large area array (50 × 50 μm2) containing approximately 5,000 NWs. Figure 1 gives a representative 30o-tilted SEM image. Micro-photoluminescence (μ-PL) spectrum at room temperature exhibits a strong emission peak at a wavelength of 1060 nm, corresponding to a 20% In composition of the InGaAs core. Furthermore, the μ-PL results reveal that the use of an In0.5Ga0.5P passivation layer is crucial to minimize the surface recombination and enhance the emission efficiency of the In0.2Ga0.8As core NWs

Relative mislocalization of successively presented stimuli

When observers were asked to localize the peripheral position of a briefly presented target with respect to a previously presented comparison stimulus, they tended to judge the target as being more towards the fovea than the comparison stimulus. Three experiments revealed that the mislocalization only emerged when the comparison stimulus and the target were presented successively. Varying the temporal interval between stimuli showed that the mislocalization reversed with longer stimulus-onset asynchronies. Further, the mislocalization was increased with a decrease of the spatial distance between stimuli. These findings suggested that the mislocalization originated from local excitatory and inhibitory mechanisms. Corroborating this idea a neuronal dynamic field model was successfully developed to account for the findings.Fundação para a Ciência e a Tecnologia (FCT

Itraconazole inhibits nuclear delivery of extracellular vesicle cargo by disrupting the entry of late endosomes into the nucleoplasmic reticulum

Extracellular vesicles (EVs) are mediators of intercellular communication under bothhealthy and pathological conditions, including the induction of pro-metastatic traits,but it is not yet known how and where functional cargoes of EVs are delivered to theirtargets in host cell compartments. We have described that after endocytosis, EVsreach Rab+late endosomes and a fraction of these enter the nucleoplasmic reticu-lum and transport EV biomaterials to the host cell nucleoplasm. Their entry thereinand docking to outer nuclear membrane occur through a tripartite complex formedby the proteins VAP-A, ORP and Rab (VOR complex). Here, we report that theantifungal compound itraconazole (ICZ), but not its main metabolite hydroxy-ICZor ketoconazole, disrupts the binding of Rab to ORP–VAP-A complexes, leadingto inhibition of EV-mediated pro-metastatic morphological changes including cellmigration behaviour of colon cancer cells. With novel, smaller chemical drugs, inhi-bition of the VOR complex was maintained, although the ICZ moieties responsiblefor antifungal activity and interference with intracellular cholesterol distributionwere removed. Knowing that cancer cells hijack their microenvironment and thatEVs derived from them determine the pre-metastatic niche, small-sized inhibitors ofnuclear transfer of EV cargo into host cells could nd cancer therapeutic applications,particularly in combination with direct targeting of cancer cell

Defeating Paediatric Tuberculous Meningitis: Applying the WHO "Defeating Meningitis by 2030: Global Roadmap".

Children affected by tuberculous meningitis (TBM), as well as their families, have needs that lie at the intersections between the tuberculosis and meningitis clinical, research, and policy spheres. There is therefore a substantial risk that these needs are not fully met by either programme. In this narrative review article, we use the World Health Organization (WHO) "Defeating Meningitis by 2030: global roadmap" as a starting point to consider key goals and activities to specifically defeat TBM in children. We apply the five pillars outlined in the roadmap to describe how this approach can be adapted to serve children affected by TBM. The pillars are (i) prevention; (ii) diagnosis and treatment; (iii) surveillance; (iv) support and care for people affected by meningitis; and (v) advocacy and engagement. We conclude by calling for greater integration between meningitis and TB programmes at WHO and at national levels

Progress and gaps in climate change adaptation in coastal cities across the globe

Coastal cities are at the frontlines of climate change impacts, resulting in an urgent need for substantial adaptation. To understand whether and to what extent cities are on track to prepare for climate risks, this paper systematically assesses the academic literature to evaluate climate change adaptation in 199 coastal cities worldwide. We show that adaptation in coastal cities is rather slow, of narrow scope, and not transformative. Adaptation measures are predominantly designed based on past and current, rather than future, patterns in hazards, exposure, and vulnerability. City governments, particularly in high-income countries, are more likely to implement institutional and infrastructural responses, while coastal cities in lower-middle income countries often rely on households to implement behavioral adaptation. There is comparatively little published knowledge on coastal urban adaptation in low and middle income economies and regarding particular adaptation types such as ecosystem-based adaptation. These insights make an important contribution for tracking adaptation progress globally and help to identify entry points for improving adaption of coastal cities in the future

Development of the PSYCHS: Positive SYmptoms and Diagnostic Criteria for the CAARMS Harmonized with the SIPS

Aim: To harmonize two ascertainment and severity rating instruments commonly used for the clinical high risk syndrome for psychosis (CHR-P): the Structured Interview for Psychosis-risk Syndromes (SIPS) and the Comprehensive Assessment of At-Risk Mental States (CAARMS). Methods: The initial workshop is described in the companion report from Addington et al. After the workshop, lead experts for each instrument continued harmonizing attenuated positive symptoms and criteria for psychosis and CHR-P through an intensive series of joint videoconferences. Results: Full harmonization was achieved for attenuated positive symptom ratings and psychosis criteria, and modest harmonization for CHR-P criteria. The semi-structured interview, named Positive SYmptoms and Diagnostic Criteria for the CAARMS Harmonized with the SIPS (PSYCHS), generates CHR-P criteria and severity scores for both CAARMS and SIPS. Conclusions: Using the PSYCHS for CHR-P ascertainment, conversion determination, and attenuated positive symptom severity rating will help in comparing findings across studies and in meta-analyses