The liver histological analysis of <i>T</i>.

<div><p><b><i>gondii</i>-infected mice from different groups</b>. </p> <p>Infected mice i.p. inoculated with 10² RH tachyzoites of <i>T. gondii</i> were killed at 9-10 days p.i. (A) Representative microscopic pictures show sections from uninfected mouse treated with PBS (a and b), infected control mouse (c and d), infected mouse treated with C48/80 (e and f), and infected mouse treated with DSCG (g and h). Tachyzoites were indicated with arrows. H&E stain. (B) Quantitative analysis of the number of inflammatory foci per field in liver sections from different groups. There were 4 mice per group, and the data are representative of two experiments. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01 (compared to control). </p></div

Light photomicrographs of metachromatic MCs in mesenteries by toluidine blue staining.

<p>Infected mice i.p. inoculated with 10² RH tachyzoites of <i>T. gondii</i> from different groups were killed at 9-10 days p.i. Metachromatic MCs were evaluated in mesentery tissue from uninfected mouse treated with PBS (a), infected control mouse displaying mildly degranulated MCs (b), uninfected mouse treated with C48/80 (c) and infected mouse treated with C48/80 (d), both displaying degranulated MCs (arrows); uninfected mouse treated with DSCG (e) and infected mouse treated with DSCG (f), both displaying intact MCs. </p

The numbers of metachromatic and tryptase-positive MCs in spleen tissues from different groups expressed as MCs mm⁻².

<p>There were 4 mice per group, and the data are representative of two experiments. Statistically significant differences for comparison with the uninfected mice with PBS (**, <i>P</i> < 0.01) and for comparison with the infected controls (§, <i>P</i> < 0.01). </p

Light photomicrographs of tryptase positive-MCs in mesenteries by immunofluorescence staining.

<p>Infected mice i.p. inoculated with 10² RH tachyzoites of <i>T. gondii</i> from different groups were killed at 9-10 days p.i. MCs were evaluated in mesentery tissue from uninfected mouse treated with PBS (a), infected control mouse displaying a degranulated MC (arrow) (b), uninfected mouse treated with C48/80 (c) and infected mouse treated with C48/80 (d), both displaying degranulated MCs (arrows); uninfected mouse treated with DSCG (e) and infected mouse treated with DSCG (f), both displaying intact MCs. </p

The spleen histological analysis of <i>T</i>.

<div><p><b><i>gondii</i>-infected mice from different groups</b>. </p> <p>Infected mice i.p. inoculated with 10² RH tachyzoites of <i>T. gondii</i> were killed at 9-10 days p.i. (A) Representative microscopic pictures show sections from uninfected mouse treated with PBS (a), <i>T. gondii</i>-infected control mouse (b), <i>T. gondii</i>-infected mouse treated with C48/80 (c), and <i>T. gondii</i>-mouse treated with DSCG (d). Tachyzoites were indicated with arrows. H&E stain. (B) Histological score analysis of spleen tissues. There were 4 mice per group, and the data are representative of two experiments. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01 (compared to control). </p></div

Mice survival after infection with 10² RH tachyzoites of <i>T</i>.

<div><p><b><i>gondii</i></b>. </p> <p>Survival of naïve mice treated with PBS (open square, n=8); uninfected mice treated with C48/80 (dash, n=8); uninfected mice treated with DSCG (open upright triangle, n=8); <i>T. gondii</i>-infected control mice (filled square, n=7), <i>T. gondii</i>-infected mice with C48/80 treatment (asterisk, n=9), and <i>T. gondii</i>-infected mice with DSCG treatment (filled upright triangle, n=8). The mice were monitored for survival on a daily basis until the termination of the experiment. </p></div

Cytokine mRNA expressions in livers from different groups i.p. inoculated with 10²<i>T</i>.

<div><p><b><i>gondii</i> RH strain tachyzoites at 9-10 days p.i., using qRT-PCR</b>. </p> <p>There were 4 mice per group, and the data are representative of two experiments. Symbols indicate statistically significant differences (<i>P</i> < 0.01) for comparison with the uninfected control mice (**) and the infected controls (§), and statistically significant differences (<i>P</i> < 0.05) for comparison with the infected controls (#).</p></div

The mesentery histopathology of <i>T</i>.

<div><p><b><i>gondii</i>-infected mice from different groups</b>. </p> <p>Infected mice i.p. inoculated with 10² RH tachyzoites of <i>T. gondii</i> were killed at 9-10 days p.i. (A) Representative microscopic pictures show sections from uninfected mouse treated with PBS (a), <i>T. gondii</i>-infected control mouse (b), <i>T. gondii</i>-infected mouse treated with C48/80 (c), and <i>T. gondii</i>-infected mouse treated with DSCG (d). Tachyzoites were indicated with arrows. H&E stain. (B) Histological score analysis of mesentery tissues. There were 4 mice per group, and the data are representative of two experiments. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01 (compared to control). </p></div

Light photomicrographs of tryptase positive-MCs in spleens by immunofluorescence staining.

<p>Infected mice i.p. inoculated with 10² RH tachyzoites of <i>T. gondii</i> from different groups were killed at 9-10 days p.i. MCs were evaluated in spleen tissue from uninfected mouse treated with PBS (a), infected control mouse displaying degranulated MCs (arrows) (b), uninfected mouse treated with C48/80 (c) and infected mouse treated with C48/80 (d), both displaying degranulated MCs (arrows); uninfected mouse treated with DSCG (e) and infected mouse treated with DSCG (f), both displaying intact MCs. </p

Parasite burden of <i>T</i>.

<div><p><b><i>gondii</i> RH strain tachyzoites in the peritoneal lavage fluids and tissues</b>. </p> <p>(A) Parasite burden of <i>T. gondii</i> RH strain tachyzoites in the peritoneal lavage fluids and (B) normalized mRNA expression levels of <i>T. gondii</i> tachyzoite SAG1 gene in the spleens and livers using qRT-PCR, from different groups i.p. inoculated with 10²<i>T. gondii</i> RH strain tachyzoites at 9-10 days p.i. There were 4 mice per group, and the data are representative of two experiments. Symbols indicate statistically significant differences (<i>P</i> < 0.01) for comparison with the uninfected controls (**) and for comparison between group means (§). </p></div