A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent-0

<p><b>Copyright information:</b></p><p>Taken from "A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent"</p><p>BMC Microbiology 2005;5():56-56.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1262726.</p><p>Copyright © 2005 Takahashi and Watanabe; licensee BioMed Central Ltd.</p>ern blotting in panel C. B. Amplification of gonococcal gene by PCR. The genomic DNAs of neisserial species used for PCR were as follows: lane 1, H44/76 (); lane 2, NIID113 (IS) [49]; lane 3–13, ATCC49226, NIID54, NIID102, NIID103, NIID104, NIID105, NIID106, NIID107, NIID108, NIID109, NIID111 (). C. Southern blotting using the meningococcal gene as a probe. Two micrograms of purified chromosomal DNA digested with I were subjected to this analysis. Lane 1, H44/76 (); lane 2, NIID113 (IS) [49]; lane 3, NIID54 (); lane 4, ATCC23970 (); lane 5, ATCC13120 (); lane 6, ATCC14686 (); lane 7, ATCC25295 (); lane 8, ATCC14687 (); lane 9, ATCC14685 (); lane 10, NIID16 (); lane 11, NIID17 ()

A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent-4

<p><b>Copyright information:</b></p><p>Taken from "A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent"</p><p>BMC Microbiology 2005;5():56-56.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1262726.</p><p>Copyright © 2005 Takahashi and Watanabe; licensee BioMed Central Ltd.</p>106 were subjected to this analysis. B. RT-PCR to detect the transcripts of the gonococcal genes. The schematic figure in the box depicts the position of the primers used in this experiment (see Table 1). RT-PCR was performed without reverse transcriptase (RTase) (lanes 1 to 5) or with RTase (lanes 6 to 10). Lanes 1 and 6, H44/76 (); lanes 2 and 7, ATCC49226 (); lanes 3 and 8, NIID54 (); lanes 4 and 9, NIID103 (); lanes 5 and 10, NIID106 (). The marker in the left-most lane is X174 DNA digested with III. Primer sets used for RT-PCR are shown on the left side, and the corresponding PCR products are indicated by arrows on the right side. C. Primer extension analysis to detect the transcriptional start point of the and genes. Total RNA extracted from H44/76 (), ATCC49226 and NIID106 () was used for the primer extension with AMV reverse transcriptase XL and biotin-labeled oligonucleotide ggt-ext-2. The arrow on the right side indicates the transcriptional start site. D. Alignment of the nucleotide sequences of the upstream regions of the and genes. The sequence data have been deposited in the DDBJ/EMBL/GenBank Databases under the following Accession Numbers:strains H44/76 [DDBJ:], H114/90 [DDBJ:], strains ATCC49226 [DDBJ:], NIID54 [DDBJ:], NIID103 [DDBJ:], NIID106 [DDBJ:]. An identical nucleotide is represented as *. The transcriptional start site is shown in bold as +1. The putative -35, -10 elements and Shine-Dalgarno sequence (SD) are depicted in the box, and the ideal -35 and -10 nucleotide sequences are shown above the boxes. The previously predicted start codon (ATG) [25] and newly predicted start codon (GTG) of the meningococcal {\it ggt} gene are underlined. The amino acid sequence deduced from the putative start codon GTG (shown in bold) in the meningococcal {\it ggt} gene is also shown under the corresponding nucleotide sequences

A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent-1

<p><b>Copyright information:</b></p><p>Taken from "A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent"</p><p>BMC Microbiology 2005;5():56-56.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1262726.</p><p>Copyright © 2005 Takahashi and Watanabe; licensee BioMed Central Ltd.</p>GenBank Databases under the following Accession Numbers:strains H44/76 [DDBJ:], H114/90 [DDBJ:], 2996 [DDBJ:], NIID68 [DDBJ:], NIID76 [DDBJ:], NIID413 [DDBJ:] and NIID414 [DDBJ:]; strains ATCC49226 [DDBJ:], NIID54 [DDBJ:], NIID102 [DDBJ:], NIID103 [DDBJ:], NIID104 [DDBJ:], NIID105 [DDBJ:], NIID106 [DDBJ:], NIID107 [DDBJ:], NIID108 [DDBJ:], NIID109 [DDBJ:], NIID111 [DDBJ:]. The scale bar represents uncorrected distances, and a fit parameter is also shown. B. Alignment of the nucleotide sequences containing 4 kinds of differences in the and genes of strains H44/76 and H114/90; strains ATCC49226, NIID54, NIID103 and NIID106, respectively. Sequence identity is represented as *, polymorphism among the sequences of the 6 strains is indicated by the appropriate letter, and the absence of a base is shown by a hyphen (-). The nucleotide substitution for an ochre (Type II) mutation is shown in bold. Boxes at the Type I and Type III mutations indicate the tandem repeat. The tetranucleotide repeat in type I is also shown as a gray box. The newly deduced start codon of the meningococcal GGT is shown in underlined bold

A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent-2

<p><b>Copyright information:</b></p><p>Taken from "A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent"</p><p>BMC Microbiology 2005;5():56-56.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1262726.</p><p>Copyright © 2005 Takahashi and Watanabe; licensee BioMed Central Ltd.</p>n shown in Figure 2B. * indicates the position of the 7-bp deletion (Type III) mutation in the gene. B. Putative translated products from the corrected nucleotide sequences of the genes. The Type IV insertional mutation shown in Figure 2B is removed and the site of Type III deletion is replaced by the letter B. The site of the ochre (Type II) mutation is shown as X. The identical amino acid sequences between and are shown in a black box and amino acid sequences common to only are shown in a gray box

Change in activities of G6PDH, 6PGDH, aconitase, and ICDH in wild-type (open circles) and Δ (closed circles) cells upon the shift from photoautotrophic to photomixotrophic conditions

Cultures grown at 50 μmol photons m s were supplemented with 5 mM glucose at time 0. Samples were taken at the indicated time points, and enzymatic activities in cell extracts were determined as described in the Materials and methods. Data are the means ±SD of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3009-3018.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2504344.</p><p></p

Photosynthetic (A) and respiratory (B) activities of the wild-type and Δ cells incubated under photoautotrophic or photomixotrophic conditions for 24 h

Photosynthetic activity was measured as oxygen evolution supported by 2 mM NaHCO at 50 μmol photons m s. Respiratory activity was measured as oxygen consumption in the presence of 5 mM glucose in the dark. Data are the means ±SD of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3009-3018.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2504344.</p><p></p

Growth curve of the wild-type (open circles) and Δ (closed circles) cells in liquid BG-11 medium supplemented with 5 mM glucose

Cultures grown at 50 μmol photons m s were supplemented with glucose at time 0, and diluted every 24 h to avoid the self shading of cells.<p><b>Copyright information:</b></p><p>Taken from "Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3009-3018.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2504344.</p><p></p

Amounts of metabolites in glycolysis, the OPP pathway and the Calvin cycle

Wild-type and Δ mutant were grown photoautotrophically (white bars) or photomixotrophically (black bars) for 24 h, and then metabolite contents were quantified. Each value represents the means ±SD of three independent experiments (nmol g fresh weight).<p><b>Copyright information:</b></p><p>Taken from "Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3009-3018.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2504344.</p><p></p

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sj-xlsx-4-ajr-10.1177_19458924221132065 - Supplemental material for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment

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