Artificial Recharge of Groundwater with Recycled Municipal Wastewater in the Pajaro Valley

Abstract The Pajaro Valley, located along California’s Central Coast, is the State’s 5th most productive agricultural region. Groundwater is the main source of water for the region, and due to high agricultural demand, the Pajaro Valley is facing groundwater depletion and rapid seawater intrusion. Artificial recharge of recycled water into groundwater aquifers in other locations has proven to be an effective method of mitigating groundwater depletion and seawater intrusion while providing a sustainable water supply. Two methods of artificial recharge with recycled water exist: direct injection and surface spreading (infiltration). Case studies of both methods of recharge were analyzed to determine the benefits, issues and solutions associated with each project type. Direct injection is very effective when used as a seawater intrusion barrier to exert hydraulic pressure on seawater, preventing it from flowing inland. As evidenced by several case studies, clogging can be significant in direct injection wells, but well design and maintenance can effectively address clogging. Surface spreading basins require less engineering and have lower operating costs than direct injection, and they are able to accommodate fluctuating flows of water, unlike direct injection wells. Clogging of the infiltration basin surface is inevitable, as demonstrated by the case studies, but this type of clogging is relatively easy to remedy with regular basin maintenance. Based on the information gained from the case studies, the Pajaro Valley is a feasible basin for both direct injection, in the form of a seawater barrier, and surface spreading operations. A dual project featuring both a seawater barrier directly along the coast and a surface spreading basin further inland is recommended to provide the highest possible defense against saltwater intrusion while taking full advantage of all recycled water production to recharge the groundwater aquifer and supplement water supplies

The rate of the musculoskeletal pathology assessment for military personnel and civil population

Musculoskeletal disorders are an important cause of morbidity for civil population as well as for military personnel. Musculoskeletal disorders represent a prevalent source of patient visits, lost work time, hospitalization and disability. The three most important pain areas are those of lumbar spine, shoulders and lower extremities. We provide assessment of Anthropometric characteristics in two groups. Anthropometric characteristics of military personnel (n = 60) that leave the active service and civil population of the same age (n = 100) were collected during two year period (2011/2012). The anthropometric characteristics were evaluated for military personnel going for retirement with diagnosis of musculoskeletal pathology. The evaluated anthropometric characteristics were the height, the body mass and the anthropometric indices. Body fat ranges for standard adults were calculated according to NIH/WHO BMI guidelines. 30% of military persons with diagnosis of musculoskeletal pathology and individuals from civil population group with diagnosis of the musculoskeletal pathology have values of body mass index above the standard level One of the reasons the musculoskeletal pathology is the overweight it may be important to provide preventive measures to reduce the risk of musculoskeletal disorders linked to specific job and treatment at early stage.publishersversionPeer reviewe

Posttraumatic stress and symptom improvement in Norwegian tourists exposed to the 2004 tsunami – a longitudinal study

Background Mental health consequences of disasters are frequently studied. However, few studies have investigated symptom improvement in victims after natural disasters. This study aimed to identify predictors of 6 months post-disaster stress symptoms and to study 6 months and 24 months course of symptoms among Norwegian tourists who experienced the 2004 tsunami. Methods Norwegian tourists (≥18 years) who experienced the 2004 tsunami (n = 2468) were invited to return a postal questionnaire at two points of time. The first data set was collected at 6 months (T1, n = 899) and the second data set at 24 months post-disaster (T2, n = 1180). The population studied consisted of those who responded at both assessments (n = 674). Impact of Event Scale Revised (IES-R) was used to measure posttraumatic stress symptoms. IES–R score ≥33 (caseness) was used to identify various symptom trajectories from T1 to T2. Multiple linear regression was used to determine predictors of posttraumatic stress at T1 and to identify variables associated with symptom improvement from T1 to T2. Results The majority was identified as non-case at both assessments (57.7%), while 20.8% of the respondents were identified as case at both assessments. Symptoms at T1 were positively related to female gender, older age, unemployment, being chased or caught by the waves, witnessing death or suffering, loss of loved ones, experiencing intense fear during the disaster, low conscientiousness, neuroticism and low levels of social support. The IES-R sum score declined from 24.6 (SD = 18.5) at T1 to 22.9 (SD = 18.3) at T2, p < 0.001. Emotional stability and high IES-R scores at T1 were positively related to symptom improvement, while received social support was not. Being referred to a mental health specialist was negatively related to symptom improvement. Conclusions A significant minority (20-30%) among Norwegian tourists developed enduring posttraumatic stress symptoms in the aftermath of the 2004 tsunami. Tsunami exposure, peritraumatic fear, neuroticism and low levels of social support were the strongest predictors of posttraumatic stress at 6 months post-disaster. Decrease in posttraumatic stress was related to emotional stability and higher symptom levels at T1. Being referred to a mental health specialist did not facilitate symptom improvement

Quality of life in people with visual impairment compared with the general population

Aim The aim of the present study was to examine quality of life and its associated factors among adults with visual impairment in Norway. Subject and methods Of the 1216 adults contacted, 736 (61% response rate) participated in a cross-sectional survey. A general population probability sample served as reference (n=1792, 36% response rate). Differences between the populations were examined with independent t-tests for continuous variables and with Chi-square tests for categorical variables. Quality of life covariates were investigated with linear regression analysis. Results People with visual impairment had lower quality of life than the general Norwegian population, across all age groups (mean QOL: 6.8 versus 7.6, p<0.001) and within all age groups (all p<0.01). Compared with the general population, people with visual impairment were less likely to report the highest levels and more likely to report intermediate levels of quality of life. Higher quality of life was associated with lower onset-age of impaired vision (B=-0.11, p<0.001), having employment (B=0.36, p=0.02), lower levels of loneliness (B=-0.28, p<0.001) and higher levels of social support (B=0.40, p<0.001) and general self-efficacy (B=0.07, p<0.001). Conclusion Quality of life was lower among people with visual impairment and may be increased by promoting work participation, social inclusion, connectedness, and coping.publishedVersio

Posttraumatic stress disorder in people with visual impairment compared with the general population

Background: People with a visual impairment appear to have an increased risk of experiencing potentially traumatizing life events and possibly also subsequently developing post-traumatic stress disorder (PTSD). This study investigated the point prevalence of PTSD in people with a visual impairment compared with the general population of Norway and examined factors associated with PTSD among people with a visual impairment. Methods: A telephone-based survey was administered to a probability sample of 1216 adults with a visual impairment. Of these, 736 (61% response rate) participated. A probability sample from the general population served as a reference (n = 1792, 36% response rate). PTSD was measured with the PTSD Checklist for the DSM-5 (PCL-5), based on the currently most bothersome event reported from the Life Events Checklist for DSM-5 (LEC-5). We used the DSM-5 diagnostic guidelines to categorize participants as fulfilling the PTSD symptom criteria or not. Results: The prevalence of PTSD was higher among people with a visual impairment than in the general population, both for men (9.0% vs. 3.8%) and women (13.9% vs. 8.5%). The prevalence rates of PTSD from the illness or injury that had caused the vision loss (men 3.9%, women 2.2%) accounted for a considerable part of the difference between the populations. For women, PTSD related to sexual assaults also contributed significantly to a higher PTSD prevalence in the visually impaired versus the general population (5.2% vs. 2.2%), while for men there were no other event categories which resulted in significant differences. Among people with a visual impairment, the higher risk of PTSD was associated with lower age, female gender, having acquired the vision loss, and having other impairments in addition to the vision loss. Conclusion: The higher prevalence of PTSD in people with a visual impairment suggests that vulnerability to mental health problems is associated with serious life events. The higher incidence than in the general population is partly due to the illness or injury that had led to the vision loss and partly due to people with vision loss appearing to be more vulnerable through exposure to other types of potentially traumatizing events, such as sexual abuse.publishedVersio

Online Enlightenment: A Phidget Notification System for Online Status

This paper describes a physical device that presents online presence information in a semi-public space. The device uses a map metaphor to represent a set of connected labs, showing online instant messenger status for members of the community. Device users can combine information from the device with information from the physical environment to identify unfamiliar lab members, determine human-to-human interaction strategies, and plan meetings. The paper reports on design decisions that were considered in creating the device, supplying rationale for decisions that were made. In particular, we focus on how people integrate physical information from the world and virtual information from this (and similar) devices in the environment, reflecting on ways in which this type of device can improve communication and enhance community. We describe four envisioned usage scenarios for the device, with early feedback from people who work in the space and whose information is displayed on the device

DNA fingerprinting of Shiga-toxin producing Escherichia coli O157 based on Multiple-Locus Variable-Number Tandem-Repeats Analysis (MLVA)

BACKGROUND: The ability to react early to possible outbreaks of Escherichia coli O157:H7 and to trace possible sources relies on the availability of highly discriminatory and reliable techniques. The development of methods that are fast and has the potential for complete automation is needed for this important pathogen. METHODS: In all 73 isolates of shiga-toxin producing E. coli O157 (STEC) were used in this study. The two available fully sequenced STEC genomes were scanned for tandem repeated stretches of DNA, which were evaluated as polymorphic markers for isolate identification. RESULTS: The 73 E. coli isolates displayed 47 distinct patterns and the MLVA assay was capable of high discrimination between the E. coli O157 strains. The assay was fast and all the steps can be automated. CONCLUSION: The findings demonstrate a novel high discriminatory molecular typing method for the important pathogen E. coli O157 that is fast, robust and offers many advantages compared to current methods

Reduction and Growth Inhibition of Listeria monocytogenes by Use of Anti-Listerial Nisin, P100 Phages and Buffered Dry Vinegar Fermentates in Standard and Sodium-Reduced Cold-Smoked Salmon

Cold-smoked salmon are ready-to-eat products that may support the growth of pathogenic Listeria monocytogenes during their long shelf-life. Consumption of such contaminated products can cause fatal listeriosis infections. Another challenge and potential risk associated with CS salmon is their high levels of sodium salt. Excess dietary intake is associated with serious health complications. In the present study, anti-listerial bacteriocin (nisin), P100 bacteriophages (Phageguard L, PGL) and fermentates (Verdad N6, P-NDV) were evaluated as commercial bio-preservation strategies for increased control of L. monocytogenes in standard (with NaCl) and sodium-reduced (NaCl partially replaced with KCl) CS salmon. Treatments of CS salmon with nisin (1 ppm) and PGL (5 x 107 pfu/cm2) separately yielded significant initial reductions in L. monocytogenes (up to 0.7 log) compared to untreated samples. Enhanced additive reductions were achieved through the combined treatments of nisin and PGL. Fermentates in the CS salmon inhibited the growth of Listeria but did not lead to its eradication. The lowest levels of L. monocytogenes during storage were observed in nisin- and PGL-treated CS salmon containing preservative fermentates and stored at 4 C, while enhanced growth was observed during storage at an abusive temperature of 8 C. Evaluation of industry-processed standard and sodium-replaced CS salmon confirmed significant effects with up to 1.7 log reductions in L. monocytogenes levels after 34 days of storage of PGL- and nisin-treated CS salmon-containing fermentates. No differences in total aerobic plate counts were observed between treated (PGL and nisin) or non-treated standard and sodium-reduced CS salmon at the end of storage. The microbiota was dominated by Photobacterium, but with a shift showing dominance of Lactococcus spp. and Vagococcus spp. in fermentate-containing samples. Similar and robust reductions in L. monocytogenes can be achieved in both standard and sodium-replaced CS salmon using the bio-preservation strategies of nisin, PGL and fermentates under various and relevant processing and storage conditions.Reduction and Growth Inhibition of Listeria monocytogenes by Use of Anti-Listerial Nisin, P100 Phages and Buffered Dry Vinegar Fermentates in Standard and Sodium-Reduced Cold-Smoked SalmonpublishedVersio

Prevalence and characterization of integrons in blood culture Enterobacteriaceae and gastrointestinal Escherichia coli in Norway and reporting of a novel class 1 integron-located lincosamide resistance gene

BACKGROUND: Class 1 integrons contain genetic elements for site-specific recombination, capture and mobilization of resistance genes. Studies investigating the prevalence, distribution and types of integron located resistance genes are important for surveillance of antimicrobial resistance and to understand resistance development at the molecular level. METHODS: We determined the prevalence and genetic content of class 1 integrons in Enterobacteriaceae (strain collection 1, n = 192) and E. coli (strain collection 2, n = 53) from bloodstream infections in patients from six Norwegian hospitals by molecular techniques. Class 1 integrons were also characterized in 54 randomly selected multiresistant E. coli isolates from gastrointestinal human infections (strain collection 3). RESULTS: Class 1 integrons were present in 10.9% of the Enterobacteriaceae blood culture isolates of collection 1, all but one (S. Typhi) being E. coli. Data indicated variations in class 1 integron prevalence between hospitals. Class 1 integrons were present in 37% and 34% of the resistant blood culture isolates (collection 1 and 2, respectively) and in 42% of the resistant gastrointestinal E. coli. We detected a total of 10 distinct integron cassette PCR amplicons that varied in size between 0.15 kb and 2.2 kb and contained between zero and three resistance genes. Cassettes encoding resistance to trimethoprim and aminoglycosides were most common. We identified and characterized a novel plasmid-located integron with a cassette-bound novel gene (linF) located downstream of an aadA2 gene cassette. The linF gene encoded a putative 273 aa lincosamide nucleotidyltransferase resistance protein and conferred resistance to lincomycin and clindamycin. The deduced LinF amino acid sequence displayed approximately 35% identity to the Enterococcus faecium and Enterococcus faecalis nucleotidyl transferases encoded by linB and linB' CONCLUSIONS: The present study demonstrated an overall low and stable prevalence of class 1 integron gene cassettes in clinical Enterobacteriaceae and E. coli isolates in Norway. Characterization of the novel lincosamide resistance gene extends the growing list of class 1 integron gene cassettes that confer resistance to an increasing number of antibiotics

Biofilm forming abilities of Salmonella are correlated with persistence in fish meal- and feed factories

<p>Abstract</p> <p>Background</p> <p>Feed contaminated with <it>Salmonella </it>spp. constitutes a risk of <it>Salmonella </it>infections in animals, and subsequently in the consumers of animal products. <it>Salmonella </it>are occasionally isolated from the feed factory environment and some clones of <it>Salmonella </it>persist in the factory environment for several years. One hypothesis is that biofilm formation facilitates persistence by protecting bacteria against environmental stress, e.g. disinfection. The aim of this study was to investigate the biofilm forming potential of <it>Salmonella </it>strains from feed- and fishmeal factories. The study included 111 <it>Salmonella </it>strains isolated from Norwegian feed and fish meal factories in the period 1991–2006 of serovar Agona, serovar Montevideo, serovar Senftenberg and serovar Typhimurium.</p> <p>Results</p> <p>Significant differences were found between serovars regarding the abilities to form biofilm on polystyrene (microtiter plate assay) and in the air-liquid interface of nutrient broth (pellicle assay). Strains of serovar Agona and serovar Montevideo were good biofilm producers. In Norwegian factories, clones of these serovars have been observed to persist for several years. Most serovar Senftenberg clones appear to persist for a shorter period, and strains of this serovar were medium biofilm producers in our test systems. Strains of the serovar Typhimurium were relatively poor biofilm producers. <it>Salmonella </it>ser. Typhimurium clones have not been observed to persist even though this serovar is resident in Norwegian wild life. When classifying strains according to persistence or presumed non-persistence, persistent strains produced more biofilm than presumed non-persisting strains.</p> <p>Conclusion</p> <p>The results indicate a correlation between persistence and biofilm formation which suggests that biofilm forming ability may be an important factor for persistence of <it>Salmonella </it>in the factory environment.</p