Supplementary document for A Metasurface Cut-off Perfect Absorber in Solar Energy Wavelength Band - 6624905.pdf

Part1 and 2 of Supplementary Materia

Video_1_Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior.AVI

<p>Magnetotactic bacteria (MTB) are a diverse group of microorganisms capable of using geomagnetic fields for navigation. This magnetotactic behavior can help microorganisms move toward favorable habitats for optimal growth and reproduction. A comprehensive understanding of the magnetotactic mechanism at molecular levels requires highly efficient genomic editing tools, which remain underdeveloped in MTB. Here, we adapted an engineered CRISPR-Cas9 system for efficient inactivation of genes in a widely used MTB model strain, Magnetospirillum magneticum AMB-1. By combining a nuclease-deficient Cas9 (dCas9) and single-guide RNA (sgRNA), a CRISPR interference system was successfully developed to repress amb0994 expression. Furthermore, we constructed an in-frame deletion mutant of amb0994 by developing a CRISPR-Cas9 system. This mutant produces normal magnetosomes; however, its response to abrupt magnetic field reversals is faster than wild-type strain. This behavioral difference is probably a consequence of altered flagella function, as suggested with our dynamics simulation study by modeling M. magneticum AMB-1 cell as an ellipsoid. These data indicate that, Amb0994 is involved in the cellular response to magnetic torque changes via controlling flagella. In summary, this study, besides contributing to a better understanding of magnetotaxis mechanism, demonstrated the CRISPR-(d)Cas9 system as a useful genetic tool for efficient genome editing in MTB.</p

Table_1_Serum fatty acid profiles associated with metabolic risk in women with polycystic ovary syndrome.xlsx

PurposeDyslipidemia is a feature of polycystic ovary syndrome (PCOS) that may augment metabolic disturbances. Serum fatty acids are important biomedical indicators of dyslipidemia. The aim of this study was to determine the distinct serum fatty acids in various PCOS subtypes and their association with metabolic risk in women with PCOS.MethodsFatty acids in the serum of 202 women with PCOS were measured using gas chromatography-mass spectrometry. Fatty acids were compared between PCOS subtypes and correlated with glycemic parameters, adipokines, homocysteine, sex hormones, and sex hormone-binding globulin (SHBG).ResultsThe levels of total monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) in the reproductive subtype of PCOS were lower than those in the metabolic subtype. Docosahexaenoic acid, a PUFA, was associated with higher SHBG after correction for multiple comparisons. Eighteen species of fatty acids emerged as potential biomarkers associated with the metabolic risk factors measured, independent of body mass index (BMI). Among them, myristic acid (C14:0), palmitoleic acid (C16:1), oleic acid (C18:1n-9C), cis-vaccenic acid (C18:1n-7), and homo-gamma-linolenic acid (C20:3n-6) were the strongest lipid species that were consistently associated with metabolic risk factors, particularly insulin-related parameters in women with PCOS. As for adipokines, 16 fatty acids were positively associated with serum leptin. Among them, C16:1 and C20:3n-6were significantly associated with leptin levels.ConclusionOur data demonstrated that a distinct fatty acid profile comprising high C14:0, C16:1, C18:1n-9C, C18:1n-7, and C20:3n-6levels is associated with metabolic risk in women with PCOS, independent of BMI.</p

Enhanced Directional Emission from Monolayer WSe₂ Integrated onto a Multiresonant Silicon-Based Photonic Structure

Two-dimensional transition-metal dichalcogenides such as WSe₂ show great promise as versatile atomic-scale light sources for on-chip applications due to their advanced optoelectronic properties and compatibility with a silicon photonics platform. However, the sub-nanometer thickness of such active materials limits their emission efficiency. Hence, new approaches to simultaneously enhance the emission and control its directionality are required. Here, we demonstrate enhanced and directional emission from a WSe₂ monolayer integrated onto a silicon photonic structure. This is achieved by coupling of the WSe₂ layer to a multiresonant silicon grating-waveguide structure. The interaction with the multiple resonant modes supported by the structure provides simultaneous excitation and emission enhancement, while the dispersion of the modes further routes the emission into specified directions. In addition, our hybrid structure offers the opportunity for ultrafast emission modulation, owing to the reduced emission lifetime of WSe₂. Such a silicon-based hybrid platform is fully scalable and promising as efficient chip-integrated and spatially multiplexed light sources

Animate field of 1.1THz

The broadband mechanism in the high-frequency band results from the coupling of Mie resonances in dielectric wires excited by the incident wave to the graphene plasmon resonances. In this visualization, we take the animate field of 1.1 THz for example to show the absorption process

Caspase polymorphisms and prognosis of hepatocellular carcinoma

<div><p>The aim of our study was to determine the impact of genetic polymorphisms in the caspase (CASP) genes on prognosis of hepatocellular carcinoma (HCC). We genotyped 7 potentially functional polymorphisms in <i>CASP3</i>, <i>CASP7</i>, <i>CASP8</i>, <i>CASP9</i>, <i>CASP10</i> genes in 362 HCC patients of receiving surgical resection of HCC tumor. The associations of genotype and haplotype with overall survival (OS) and disease free survival (DFS) were analyzed by using the Cox proportional hazards model. We found that the <i>CASP9</i> rs4645981 C allele was significantly associated with positive effect on DFS (<i>P</i> = 0.011 and 0.016 for CT+CC vs. TT in univariate and multivariate analysis, respectively), CT genotype was associated with a better OS of HCC than the TT genotype both in univariate and multivariate analysis (<i>P</i> = 0.048 and 0.041, respectively). Moreover, the <i>CASP3</i> rs2705897 GT genotype showed marginally significant association with decreased OS and DFS, compared with the GG genotype. One haplotype TT/TG in <i>CASP3</i> (constructed by rs12108497 T>C and rs2705897 T>G) was significantly associated with decreased OS and DFS, compared to the common haplotype TT/TT both in univariate analysis (<i>P</i> = 0.021 and 0.026, respectively) and multivariate analysis (<i>P</i> = 0.025 and 0.030, respectively). The haplotype GT/GT in <i>CASP9</i> (constructed by rs4645978 A>G and rs4645981 C>T) was significantly associated with decreased DFS both in univariate and multivariate analysis (<i>P</i> = 0.012 and 0.010, respectively). In conclusion, the <i>CASP9</i> rs4645981 polymorphism, <i>CASP3</i> and <i>CASP9</i> haplotypes may be useful prognosis markers for HCC patients with surgical resection of tumor.</p></div

Kaplan-Meier survival curves of <i>CASP9</i>_haplotype with disease free survival of HCC patients.

<p>Kaplan-Meier survival curves of <i>CASP9</i>_haplotype with disease free survival of HCC patients.</p

Kaplan-Meier survival curves of <i>CASP9</i> rs4645981 with clinical outcomes of 362 HCC patients.

<p>(A) disease free survival (DFS) (B) overall survival (OS).</p

Kaplan-Meier survival curves of <i>CASP3</i>_haplotype with clinical outcomes of HCC patients.

<p>(A) overall surviva<i>l</i> (OS), and (B) disease free survival (DFS).</p

Univariate and multivariate Cox regression analysis of genotypes in HCC patients.

<p>Univariate and multivariate Cox regression analysis of genotypes in HCC patients.</p