Cell seeding chamber for bone graft substitutes

There is an increasing demand for bone graft substitutes that are used as osteoconductive scaffolds in the treatment of bone defects and fractures. Achieving optimal bone regeneration requires initial cell seeding of the scaffolds prior to implantation. The cell seeding chamber is a closed assembly. It works like a sandglass. The position of the scaffold is between two reservoirs containing the fluid (e. g. blood). The fluid at the upper reservoir flows through the scaffold driven by gravity. Fluid is collected at the lower reservoir. If the upper reservoir is empty the whole assembly turned and the process starts again. A new compact cell seeding chamber for initial cell seeding has been developed that can be used in the operating theater

New computer system simplifies programming of mathematical equations

Automatic Mathematical Translator /AMSTRAN/ permits scientists or engineers to enter mathematical equations in their natural mathematical format and to obtain an immediate graphical display of the solution. This automatic-programming, on-line, multiterminal computer system allows experienced programmers to solve nonroutine problems

Microlensing events from the 11-year observations of the Wendelstein Calar Alto Pixellensing Project

We present the results of the decade-long M31 observation from the Wendelstein Calar Alto Pixellensing Project (WeCAPP). WeCAPP has monitored M31 from 1997 till 2008 in both R- and I-filters, thus provides the longest baseline of all M31 microlensing surveys. The data are analyzed with the difference imaging analysis, which is most suitable to study variability in crowded stellar fields. We extracted light curves based on each pixel, and devised selection criteria that are optimized to identify microlensing events. This leads to 10 new events, and sums up to a total of 12 microlensing events from WeCAPP, for which we derive their timescales, flux excesses, and colors from their light curves. The color of the lensed stars fall between (R-I) = 0.56 to 1.36, with a median of 1.0 mag, in agreement with our expectation that the sources are most likely bright, red stars at post main-sequence stage. The event FWHM timescales range from 0.5 to 14 days, with a median of 3 days, in good agreement with predictions based on the model of Riffeser et al. (2006).Comment: 44 pages, 16 figures, 5 tables. ApJ accepte

Species- and organ-specificity of secretory proteins derived from human prostate and seminal vesicles

Polyclonal antibodies against semenogelin (SG) isolated from human seminal vesicle secretion and acid phosphatase (PAP), β‐microseminoprotein (β‐MSP), and Prostate‐Specific Antigen (PSA) derived from human prostatic fluid, as well as a monoclonal antibody against β‐MSP were used for immunocytochemical detection of the respective antigens in different organs from different species. SG immunoreactivity was detected in the epithelium of the pubertal and adult human and in monkey seminal vesicle, ampulla of the vas deferens, and ejaculatory duct. PAP, β‐MSP, and PSA immunoreactivities were detected in the pubertal and adult human prostate and the cranial and caudal monkey prostate. With the exception of a weak PSA immunoreactivity in the proximal portions of the ejaculatory duct, none of the latter antisera reacted with seminal vesicle, ampullary, and ejaculatory duct epithelium. Among the non‐primate species studied (dog, bull, rat, guinea pig) only the canine prostatic epithelium displayed a definite immunoreactivity with the PAP antibody and a moderate reaction with the PSA antibody. No immunoreaction was seen in bull and rat seminal vesicle and canine ampulla of the vas deferens with the SG antibody. The same was true for the (ventral) prostate of rat, bull, and dog for β‐MSP. The epithelium of the rat dorsal prostate showed a slight cross‐reactivity with the monoclonal antibody against β‐MSP and one polyclonal antibody against PSA. The findings indicate a rather strict species‐dependent expression of human seminal proteins which show some similarities in primates, but only marginal relationship to species with different physiology of seminal fluid

Weak Lensing Analysis of the z~0.8 cluster CL 0152-1357 with the Advanced Camera for Surveys

We present a weak lensing analysis of the X-ray luminous cluster CL 0152-1357 at z~0.84 using HST/ACS observations. The unparalleled resolution and sensitivity of ACS enable us to measure weakly distorted, faint background galaxies to the extent that the number density reaches ~175 arcmin^-2. The PSF of ACS has a complicated shape that also varies across the field. We construct a PSF model for ACS from an extensive investigation of 47 Tuc stars in a modestly crowded region. We show that this model PSF excellently describes the PSF variation pattern in the cluster observation when a slight adjustment of ellipticity is applied. The high number density of source galaxies and the accurate removal of the PSF effect through moment-based deconvolution allow us to restore the dark matter distribution of the cluster in great detail. The direct comparison of the mass map with the X-ray morphology from Chandra observations shows that the two peaks of intracluster medium traced by X-ray emission are lagging behind the corresponding dark matter clumps, indicative of an on-going merger. The overall mass profile of the cluster can be well described by an NFW profile with a scale radius of r_s =309+-45 kpc and a concentration parameter of c=3.7+-0.5. The mass estimates from the lensing analysis are consistent with those from X-ray and Sunyaev-Zeldovich analyses. The predicted velocity dispersion is also in good agreement with the spectroscopic measurement from VLT observations. In the adopted WMAP cosmology, the total projected mass and the mass-to-light ratio within 1 Mpc are estimated to be 4.92+-0.44 10^14 solar mass and 95+-8 solar mass/solar luminosity, respectively.Comment: Accepted for publication in Astrophysical Journal. 58 pages, 26 figures. Figures have been degraded to meet size limit; a higher resolution version available at http://acs.pha.jhu.edu/~mkjee/ms_cl0152.pd

The Wendelstein Calar Alto Pixellensing Project (WeCAPP): the M31 Nova catalogue

We present light curves from the novae detected in the long-term, M31 monitoring WeCAPP project. The goal of WeCAPP is to constrain the compact dark matter fraction of the M31 halo with microlensing observations. As a by product we have detected 91 novae benefiting from the high cadence and highly sensitive difference imaging technique required for pixellensing. We thus can now present the largest CCD and optical filters based nova light curve sample up-to-date towards M31. We also obtained thorough coverage of the light curve before and after the eruption thanks to the long-term monitoring. We apply the nova taxonomy proposed by Strope et al. (2010) to our nova candidates and found 29 S-class novae, 10 C-class novae, 2 O-class novae and 1 J-class nova. We have investigated the universal decline law advocated by Hachichu and Kato (2006) on the S-class novae. In addition, we correlated our catalogue with the literature and found 4 potential recurrent novae. Part of our catalogue has been used to search for optical counter-parts of the super soft X-ray sources detected in M31 (Pietsch et al. 2005). Optical surveys like WeCAPP, and coordinated with multi-wavelength observation, will continue to shed light on the underlying physical mechanism of novae in the future.Comment: 15 pages, 15 figures, 7 tables, A&A accepted for publication. The appendix is stored in the Data Conservanc

Configuration development study of the X-24C hypersonic research airplane

Bottom line results were made of a three-phase study to determine the feasibility of designing, building, and operating, and maintaining an air-launched high performance aircraft capable of cruising at speeds up to Mach 8 for short durations. The results show that Lockalloy heat-sink structure affords the capability for a 'work-horse' vehicle which can serve as an excellent platform for this research. It was further concluded that the performance of a blended wing body configuration surpassed that of a lifting body design for typical X-24C missions. The cost of a two vehicle program, less engines, B-52 modification and contractor support after delivery, can be kept within $70M (in Jan. 1976 dollars)

Large-Scale QSO-Galaxy Correlations and Weak Lensing

Several recent studies show that bright, intermediate and high redshift optically and radio selected QSOs are positively correlated with nearby galaxies on a range of angular scales up to a degree. Obscuration by unevenly distributed Galactic dust can be ruled out as the cause, leaving weak statistical lensing as the physical process responsible. However the amplitude of correlations on < 1 degree scales is at least a factor of a few larger than lensing model predictions. A possible way to reconcile the observations and theory is to revise the weak lensing formalism. We extend the standard lensing formulation to include the next higher order term (second order) in the geodesic equation of motion for photons. We derive relevant equations applicable in the weak lensing regime, and discuss qualitative properties of the updated formulation. We then perform numerical integrations of the revised equation and study the effect of the extra term using two different types of cosmic mass density fluctuations. We find that nearby large-scale coherent structures increase the amplitude of the predicted lensing-induced correlations between QSOs and foreground galaxies by ~ 10% (not a factor of several required by observations), while the redshift of the optimal, i.e. `most correlated' structures is moved closer to the observer compared to what is predicted using the standard lensing equation.Comment: extended Section 2; 20 pages, including 4 figures, accepted to Ap

Curr Opin Chem Biol

Technological innovations and novel applications have greatly advanced the field of protein microarrays. Over the past two years, different types of protein microarrays have been used for serum profiling, protein abundance determinations, and identification of proteins that bind DNA or small compounds. However, considerable development is still required to ensure common quality standards and to establish large content repertoires. Here, we summarize applications available to date and discuss recent technological achievements and efforts on standardization