Gas chromatography : a practical course /

Translation of 2nd ed. 1986.Includes bibliographical references and index

Jahrbuch des Archivs der deutschen Jugendbewegung. Dritter Band 1971

Rückblicke auf die Historische Jugendbewegung bis 1933 und ihre Protagoniste

3D-Segmentierungstechniken und vektorwertige Bewertungsfunktionen für symbolisches Protein-Protein-Docking

Ackermann F, Herrmann G, Posch S, Sagerer G. 3D-Segmentation and Vectorvalued Scoring Functions for Symbolic Docking of Proteins. In: Schomburg D, Lessel U, eds. Bioinformatics : from nucleic acids and proteins to cell metabolism ; contributions to the Conference on "Bioinformatics", October 9 to 11, 1995, Braunschweig, Germany. GBF Monographs. Vol 18. Weinheim: VCH Publishers; 1995: 105-124

Treatment with recombinant human bone morphogenetic protein 7 leads to a transient induction of neutralizing autoantibodies in a subset of patients

Background: Recombinant human bone morphogenetic protein 7 (rhBMP7) is applied for treatment of bone fractures, especially tibial non-unions. Its application may induce autoantibodies (aAB) affecting the targeted and endogenous signaling pathways and in turn negatively impact treatment efficacy. Methods: Novel and sensitive assays for the quantification of BMP7-aAB and BMP2-aAB were established and used to analyze serum samples from healthy controls (n = 100 men, n = 100 women) and patients with long bone fracture (n = 265) treated or not with rhBMP7. Sera from three to nine time points per patient were available and enabled the evaluation of aAB over a time course of up to one year. Functional activity of the BMP-aAB was tested with a BMP-responsive cell-based reporter assay. Consolidation of the fracture was evaluated as clinical outcome potentially affected by BMP7-aAB. Results: Prevalence of BMP7-aAB and BMP2-aAB was 1–2.5% in non-treated patients or healthy controls. The rhBMP7 treatment induced a transient increase in BMP7-aAB in a subset of patients, returning to non-detectable levels within six months. IgG from BMP7-aAB positive sera inhibited dose dependently the BMP7-reporter gene activity, whereas control sera were without effect. Successful consolidation of the fracture was observed in the majority of both aAB-positive and aAB-negative patients. General significance: We conclude that BMP7-aAB can be detected as natural aAB in healthy subjects, and are transiently induced by rhBMP7 therapy in a subset of patients. The aAB are capable of antagonizing BMP7 signaling in vitro, but do not preclude treatment success in patients

Protein Docking Combining Symbolic Descriptions of Molecular Surfaces and Grid-Based Scoring Functions

Ackermann F, Herrmann G, Kummert F, Posch S, Sagerer G, Schomburg D. Protein Docking Combining Symbolic Descriptions of Molecular Surfaces and Grid-Based Scoring Functions. In: Rawlings C, Clark D, Altman R, Hunter L, Lengauer T, Wodak S, eds. Proceedings Third International Conference on Intelligent Systems for Molecular Biology. Vol 3. Menlo Park: AAAI Press; 1995: 3-11.With the growing number of known 3D protein structures, computing systems, that can predict where two protein molecules interact with each other is becoming of increasing interest. A system is presented, integrating preprocessing like the computation of molecular surfaces, segmentation, and searching for complementarity in the general framework of a pattern analyzing semantic network (ERNEST). The score of coarse symbolic computations is used by the problem independent control strategy of ERNEST to guide a more detailed analysis considering steric clash and judgements based on gridâbased surface representations. Successfull examples of the docking system are discussed that compare well with other approaches