1,045 research outputs found
Cooperation between Prostaglandin E2 and Epidermal Growth Factor Receptor in Cancer Progression: A Dual Target for Cancer Therapy
It is recognized that prostaglandin E2 (PGE2) is one key lipid mediator involved in chronic inflammation, and it is directly implicated in tumor development by regulating cancer cell growth and migration, apoptosis, epithelialâmesenchymal transition, angiogenesis, and immune escape. In addition, the expression of the enzymes involved in PGE2 synthesis, cyclooxygenase 2 (COX-2) and microsomal prostaglandin E synthase 1 (mPGES1), positively correlates with tumor progression and aggressiveness, clearly indicating the crucial role of the entire pathway in cancer. Moreover, several lines of evidence suggest that the COX2/mPGES1/PGE2 inflammatory axis is involved in the modulation of epidermal growth factor receptor (EGFR) signaling to reinforce the oncogenic drive of EGFR activation. Similarly, EGFR activation promotes the induction of COX2/mPGES1 expression and PGE2 production. In this review, we describe the interplay between COX2/mPGES1/PGE2 and EGFR in cancer, and new therapeutic strategies that target this signaling pathway, to outline the importance of the modulation of the inflammatory process in cancer fighting. © 2023 by the authors
Finite size mean-field models
We characterize the two-site marginals of exchangeable states of a system of
quantum spins in terms of a simple positivity condition. This result is used in
two applications. We first show that the distance between two-site marginals of
permutation invariant states on N spins and exchangeable states is of order
1/N. The second application relates the mean ground state energy of a
mean-field model of composite spins interacting through a product pair
interaction with the mean ground state energies of the components.Comment: 20 page
Dbl oncogene expression in MCF-10 A epithelial cells disrupts mammary acinar architecture, induces EMT and angiogenic factor secretion.
The proteins of the Dbl family are guanine nucleotide exchange factors (GEFs) of Rho GTPases and are known to be involved in cell growth regulation. Alterations of the normal function of these proteins lead to pathological processes such as developmental disorders, neoplastic transformation, and tumor metastasis. We have previously demonstrated that expression of Dbl oncogene in lens epithelial cells modulates genes encoding proteins involved in epithelial-mesenchymal-transition (EMT) and induces angiogenesis in the lens. Our present study was undertaken to investigate the role of Dbl oncogene in epithelial cells transformation, providing new insights into carcinoma progression. To assess how Dbl oncogene can modulate EMT, cell migration, morphogenesis, and expression of pro-apoptotic and angiogenic factors we utilized bi- and three-dimensional cultures of MCF-10âA cells. We show that upon Dbl expression MCF-10âA cells undergo EMT. In addition, we found that Dbl overexpression sustain
Anomalous scaling due to correlations: Limit theorems and self-similar processes
We derive theorems which outline explicit mechanisms by which anomalous
scaling for the probability density function of the sum of many correlated
random variables asymptotically prevails. The results characterize general
anomalous scaling forms, justify their universal character, and specify
universality domains in the spaces of joint probability density functions of
the summand variables. These density functions are assumed to be invariant
under arbitrary permutations of their arguments. Examples from the theory of
critical phenomena are discussed. The novel notion of stability implied by the
limit theorems also allows us to define sequences of random variables whose sum
satisfies anomalous scaling for any finite number of summands. If regarded as
developing in time, the stochastic processes described by these variables are
non-Markovian generalizations of Gaussian processes with uncorrelated
increments, and provide, e.g., explicit realizations of a recently proposed
model of index evolution in finance.Comment: Through text revision. 15 pages, 3 figure
The small GTPase Rab29 is a common regulator of immune synapse assembly and ciliogenesis
Acknowledgements We wish to thank Jorge GalĂĄn, Gregory Pazour, Derek Toomre, Giuliano Callaini, Joel Rosenbaum, Alessandra Boletta and Francesco Blasi for generously providing reagents and for productive discussions, and Sonia Grassini for technical assistance. The work was carried out with the financial support of Telethon (GGP11021) and AIRC.Peer reviewedPostprin
Optimization of organotypic cultures of mouse spleen for staining and functional assays
By preserving cell viability and three-dimensional localization, organotypic culture stands out among the newest frontiers of cell culture. It has been successfully employed for the study of diseases among which neoplasias, where tumoral cells take advantage of the surrounding stroma to promote their own proliferation and survival. Organotypic culture acquires major importance in the context of the immune system, whose cells cross-talk in a complex and dynamic fashion to elicit productive responses. However, organotypic culture has been as yet poorly developed for and applied to primary and secondary lymphoid organs. Here we describe in detail the development of a protocol suitable for the efficient cutting of mouse spleen, which overcomes technical difficulties related to the peculiar organ texture, and for optimized organotypic culture of spleen slices. Moreover, we used microscopy, immunofluorescence, flow cytometry and qRT-PCR to demonstrate that the majority of cells residing in spleen slices remain alive and maintain their original location in the organ architecture for several days after cutting. The development of this protocol represents a significant technical improvement in the study of the lymphoid microenvironment in both physiological and pathological conditions involving the immune system
U and Th content in the Central Apennines continental crust: a contribution to the determination of the geo-neutrinos flux at LNGS
The regional contribution to the geo-neutrino signal at Gran Sasso National
Laboratory (LNGS) was determined based on a detailed geological, geochemical
and geophysical study of the region. U and Th abundances of more than 50
samples representative of the main lithotypes belonging to the Mesozoic and
Cenozoic sedimentary cover were analyzed. Sedimentary rocks were grouped into
four main "Reservoirs" based on similar paleogeographic conditions and
mineralogy. Basement rocks do not outcrop in the area. Thus U and Th in the
Upper and Lower Crust of Valsugana and Ivrea-Verbano areas were analyzed. Based
on geological and geophysical properties, relative abundances of the various
reservoirs were calculated and used to obtain the weighted U and Th abundances
for each of the three geological layers (Sedimentary Cover, Upper and Lower
Crust). Using the available seismic profile as well as the stratigraphic
records from a number of exploration wells, a 3D modelling was developed over
an area of 2^{\circ}x2^{\circ} down to the Moho depth, for a total volume of
about 1.2x10^6 km^3. This model allowed us to determine the volume of the
various geological layers and eventually integrate the Th and U contents of the
whole crust beneath LNGS. On this base the local contribution to the
geo-neutrino flux (S) was calculated and added to the contribution given by the
rest of the world, yielding a Refined Reference Model prediction for the
geo-neutrino signal in the Borexino detector at LNGS: S(U) = (28.7 \pm 3.9) TNU
and S(Th) = (7.5 \pm 1.0) TNU. An excess over the total flux of about 4 TNU was
previously obtained by Mantovani et al. (2004) who calculated, based on general
worldwide assumptions, a signal of 40.5 TNU. The considerable thickness of the
sedimentary rocks, almost predominantly represented by U- and Th- poor
carbonatic rocks in the area near LNGS, is responsible for this difference.Comment: 45 pages, 5 figures, 12 tables; accepted for publication in GC
De Finetti theorem on the CAR algebra
The symmetric states on a quasi local C*-algebra on the infinite set of
indices J are those invariant under the action of the group of the permutations
moving only a finite, but arbitrary, number of elements of J. The celebrated De
Finetti Theorem describes the structure of the symmetric states (i.e.
exchangeable probability measures) in classical probability. In the present
paper we extend De Finetti Theorem to the case of the CAR algebra, that is for
physical systems describing Fermions. Namely, after showing that a symmetric
state is automatically even under the natural action of the parity
automorphism, we prove that the compact convex set of such states is a Choquet
simplex, whose extremal (i.e. ergodic w.r.t. the action of the group of
permutations previously described) are precisely the product states in the
sense of Araki-Moriya. In order to do that, we also prove some ergodic
properties naturally enjoyed by the symmetric states which have a
self--containing interest.Comment: 23 pages, juornal reference: Communications in Mathematical Physics,
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