Additional file 2 of The V protein in oncolytic Newcastle disease virus promotes HepG2 hepatoma cell proliferation at the single-cell level

Additional file 2

Additional file 1 of The V protein in oncolytic Newcastle disease virus promotes HepG2 hepatoma cell proliferation at the single-cell level

Additional file 1

Bioinspired Fluorescent Nanosheets for Rapid and Sensitive Detection of Organic Pollutants in Water

Detection of organic pollutants in aqueous media is crucial for guaranteeing water safety. Conventional methods for organic pollutant detection suffer from time-consuming operation procedures (on the order of hours) and expensive devices. Inspired by dog noses, herein, we constructed self-assembled fluorescent nanosheets for rapid and sensitive detection of organic pollutants based on the grasp-report strategy. Tetraphenylethene decorated cyclodextrins (TPE-CDs) self-assembled into nanosheets with hydrophobic TPE layers sandwiched between two hydrophilic cyclodextrin layers. The hydrophobic cavity of the outer cyclodextrin layers grasped and collected organic pollutants, and subsequently transported them to the TPE layers and quenched the fluorescence emission of TPE layers. Such nanosheets allowed rapid detection of xylene (on the order of seconds) at a concentration of 5 μg/L. With the merits of the ease of synthesis, simple operation, and high sensitivity, the fluorescent nanomaterials provide a promising candidate for rapid and sensitive detection of organic pollutants

Controllable Synthesis, Magnetic Properties, and Enhanced Photocatalytic Activity of Spindlelike Mesoporous α-Fe₂O₃/ZnO Core–Shell Heterostructures

Mesoporous spindlelike iron oxide/ZnO core–shell heterostructures are successfully fabricated by a low-cost, surfactant-free, and environmentally friendly seed-mediate strategy with the help of postannealing treatment. The material composition and stoichiometry, as well as these magnetic and optical properties, have been examined and verified by means of high-resolution transmission electron microscopy and X-ray diffraction, the thickness of ZnO layer can be simply tailored by the concentration of zinc precursor. Considering that both α-Fe₂O₃ and ZnO are good photocatalytic materials, we have investigated the photodegradation performances of the core–shell heterostructures using organic dyes Rhodamin B (RhB). It is interesting to find that the as-obtained iron oxides/ZnO core–shell heterostructures exhibited enhanced visible light or UV photocatalytic abilities, remarkably superior to the as-used α-Fe₂O₃ seeds and commercial TiO₂ products (P25), mainly owing to the synergistic effect between the narrow and wide bandgap semiconductors and effective electron–hole separation at the interfaces of iron oxides/ZnO

Mild and Regioselective <i>N</i>‑Alkylation of 2‑Pyridones in Water

A mild and regioselective <i>N</i>-alkylation reaction of 2-pyridones in water has been developed. Tween 20 (2% w/w) was added to create a micellar system for improved solubility of starting materials, which leads to enhanced reaction rates. The protocol demonstrated a wide substrate scope with good isolated yields (40–94%) for all of the 24 examples evaluated. High regioselectivity favoring <i>N</i>-alkylation over <i>O</i>-alkylation was observed for benzyl halides (>5:1), primary alkyl halides (>6:1), and bulky and less reactive secondary alkyl halides (>2.4:1)

Additional file 1: Figure S1. of Zinc Oxide Coating Effect for the Dye Removal and Photocatalytic Mechanisms of Flower-Like MoS2 Nanoparticles

Supporting information is available online from the Wiley Online Library or from the author. (DOCX 1281 kb

Plasma Sphingolipids as Potential Indicators of Hepatic Necroinflammation in Patients with Chronic Hepatitis C and Normal Alanine Aminotransferase Level

<div><p>Accurate estimation of hepatic necroinflammation caused by chronic hepatitis C (CHC) is crucial for prediction of prognosis and design of therapeutic strategy, which is particularly true for CHC patients with normal alanine aminotransferase (ALT) level. Recent studies have shown that sphingolipids have a close relationship with hepatitis C virus infection. The present study aimed to identify plasma sphingolipids related to hepatic necroinflammation. We included 120 treatment-naïve CHC patients and 64/120 had normal ALT levels (<40 U/L). CHC patients who underwent liver biopsies were subjected to Scheuer scoring analysis for scope of hepatic inflammation. Plasma sphingolipids were detected by high-performance liquid chromatography tandem mass spectrometry. Our results showed 44 plasma sphingolipids were detected altogether. Of all detected sphingolipids, hexosylceramide (HexCer) (d18∶1/22∶0) and HexCer (d18∶1/24∶0) showed a significant difference among G0/G1, G2, and G3/G4 (<i>P</i><0.05). For identifying hepatic necroinflammation (G≥2), after adjusting other factors, the odds ratio (OR) of HexCer (d18∶1/22∶0) reached 1.01 (95% confidence interval [CI]: 1.00–1.02). Furthermore, the area under the curve (AUC) of HexCer (d18∶1/22∶0) was 0.7 (<i>P</i> = 0.01) and approached that of ALT (AUC = 0.78). However, in CHC patients with normal ALT, HexCer (d18∶1/22∶0) was an independent factor (OR: 1.02, 95% CI: 1.01–1.03) to identify the hepatic necroinflammation (G≥2). HexCer (d18∶1/22∶0) not only showed the largest AUC (0.78, <i>P</i> = 0.001), but also exhibited the highest specificity of all indicators. These results indicate that plasma HexCer (d18∶1/22∶0) is a potential indicator to distinguish hepatic necroinflammation in CHC patients. For CHC with normal ALT, the ability of HexCer (d18∶1/22∶0) to distinguish hepatic necroinflammation might be superior to conventional serum indicators.</p></div

Plasma sphingolipids with significant difference by hepatic inflammation grades in patients with CHC.

<p>Plasma sphingolipids with significant difference by hepatic inflammation grades in patients with CHC.</p

ROC analysis of indicators related to identification of necroinflammation (G≥2) in CHC patients with normal ALT levels.

<p>The indicators whose <i>P</i> values of AUC were less than 0.05 were listed in the Table.</p><p>AUC: area under curve; CI: confidence interval; Se: sensitivity; Sp: specificity; PLR: positive likelihood ratio; NLR: negative likelihood ratio; HexCer: hexosylceramide; AST: aspartate aminotransferase; TBIL: total bilirubin; PTA: prothrombin activity; GGT: γ-glutamyl transpeptidase.</p

Indicators related to necroinflammation (G≥2) in CHC patients with normal ALT in univariate and multivariate analyses.

<p>Data are means ± SD or number of patients (%).</p><p><i>P</i> values are acquired by independent-samples <i>t</i> test or the Mann-Whitney test for continuous variables, and by Pearson χ² for categoric variables.</p><p>OR: odds ratio; CI: confidence interval; AST: aspartate aminotransferase; TBIL: total bilirubin; DBIL: direct bilirubin; TP: total protein; GGT: γ-glutamyl transpeptidase; ALP, alkaline phosphatase; TBA: total bile acid; PTA: prothrombin activity; Cer: ceramide; HexCer: hexosylceramide; dhCer: dihydroceramide.</p