Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers-2

<p><b>Copyright information:</b></p><p>Taken from "Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers"</p><p>http://www.virologyj.com/content/4/1/92</p><p>Virology Journal 2007;4():92-92.</p><p>Published online 24 Sep 2007</p><p>PMCID:PMC2099425.</p><p></p>mplicon size: 1059 bp), FA1-L/FA1-L' and FA1-R (amplicon size: 1014 bp), FA4-L/FA4-L' and FA4-R (amplicon size: 1072 bp), FA2-L and FA2-R (amplicon size: 1074 bp) primer pairs respectively. Sample 3~7 are for full length genome amplification primers (WA-L and WA-R) testing (amplicon size: 3181 bp)

Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers-3

<p><b>Copyright information:</b></p><p>Taken from "Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers"</p><p>http://www.virologyj.com/content/4/1/92</p><p>Virology Journal 2007;4():92-92.</p><p>Published online 24 Sep 2007</p><p>PMCID:PMC2099425.</p><p></p>leotide, otherwise outputs a '-'. The cutoff was set to (0.05, 1), and the step length is 0.05. The frequency is listed in the left box and the nucleotides are in the right box

Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers-1

<p><b>Copyright information:</b></p><p>Taken from "Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers"</p><p>http://www.virologyj.com/content/4/1/92</p><p>Virology Journal 2007;4():92-92.</p><p>Published online 24 Sep 2007</p><p>PMCID:PMC2099425.</p><p></p>1059 bp), FA4-L/FA4-L' and FA4-R (amplicon size: 1072 bp) in red arrows represent the four sets of walking primers for fragment amplification. Here we select "CTTTTTC" of X ORF as the start point. FA1-L' and FA4-L' are degenerate primers

Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers-0

<p><b>Copyright information:</b></p><p>Taken from "Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers"</p><p>http://www.virologyj.com/content/4/1/92</p><p>Virology Journal 2007;4():92-92.</p><p>Published online 24 Sep 2007</p><p>PMCID:PMC2099425.</p><p></p>leotide, otherwise outputs a '-'. The cutoff was set to (0.05, 1), and the step length is 0.05. The frequency is listed in the left box and the nucleotides are in the right box