Appendix A. Sample sites in Twelvemile Creek.

Sample sites in Twelvemile Creek

Index Files

Index File

Appendix C. Targeted PCB analytes and methods for extraction and analysis of samples by GC-ECD.

Targeted PCB analytes and methods for extraction and analysis of samples by GC-ECD

Appendix B. Summary PCB, 15N, and 13C for stream and riparian food webs.

Summary PCB, 15N, and 13C for stream and riparian food webs

Cyprinella hybrid zone data 2008_2011

Phenotypic, mtDNA haplotype, and multilocus genotype data for Cyprinella collected in 2008 and 2011

Appendix A. Location of study area and sampling sites.

Location of study area and sampling sites

Appendix B. Lake Hartwell water elevation 1962–2007.

Lake Hartwell water elevation 1962–2007

Trends in southern Rocky Mountain lake thermal regimes.

<p>Example plots of thermal change (1988–2085) and the uncertainty analysis for these rates of change in three thermal metrics for three representative lakes. These lakes span the range of relative thermal regimes observed which include cold (Odessa Lake; a-c), cool (Ypsilon Lake; d-f), and warm (Sandbeach Lake; g-i). Thermal metrics presented are the mean annual lake surface temperature (a,d,g), the mean summer lake surface temperature (b,e,h), and number of ice-free days (c,f,i). Green trend lines represent the upper and lower bounds of future thermal trends. These bounds are calculated using the highest and lowest projected mean weekly air temperatures from both climate models combined.</p

Thermal regimes of Rocky Mountain lakes warm with climate change

<div><p>Anthropogenic climate change is causing a wide range of stresses in aquatic ecosystems, primarily through warming thermal conditions. Lakes, in response to these changes, are experiencing increases in both summer temperatures and ice-free days. We used continuous records of lake surface temperature and air temperature to create statistical models of daily mean lake surface temperature to assess thermal changes in mountain lakes. These models were combined with downscaled climate projections to predict future thermal conditions for 27 high-elevation lakes in the southern Rocky Mountains. The models predict a 0.25°C·decade^-1 increase in mean annual lake surface temperature through the 2080s, which is greater than warming rates of streams in this region. Most striking is that on average, ice-free days are predicted to increase by 5.9 days ·decade^-1, and summer mean lake surface temperature is predicted to increase by 0.47°C·decade^-1. Both could profoundly alter the length of the growing season and potentially change the structure and function of mountain lake ecosystems. These results highlight the changes expected of mountain lakes and stress the importance of incorporating climate-related adaptive strategies in the development of resource management plans.</p></div

Trends in thermal conditions for southern Rocky Mountain lakes.

<p>Predicted change in lake surface temperature metrics for 27 southern Rocky Mountain lakes. Metrics were calculated using the lake-specific non-linear models of daily mean lake surface temperature, mean weekly air temperatures form SNOTEL sites, and future mean weekly air temperature predicted from climate models [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179498#pone.0179498.ref041" target="_blank">41</a>]. Rates of change ·decade^-1 were calculated by linear regression using annual measures of each metric as a function of year. Upper and lower bounds of these predictions from our uncertainty analysis are shown in parentheses.</p