Metamaterial lens of specifiable frequency-dependent focus and adjustable aperture for electron cyclotron emission in the DIII-D tokamak

Electron Cyclotron Emission (ECE) of different frequencies originates at different locations in non-uniformly magnetized plasmas. For simultaneous observation of multiple ECE frequencies from the outside edge of a toroidal plasma confinement device (e.g. a tokamak), the focal length of the collecting optics should increase with the frequency to maximize the resolution on a line of sight along the magnetic field gradient. Here we present the design and numerical study of a zoned metamaterial lens with such characteristics, for possible deployment with the 83-130 GHz ECE radiometer in the DIII-D tokamak. The lens consists of a concentric array of miniaturized element phase-shifters. These were reverse-engineered starting from the desired Gaussian beam waist locations and further optimized to account for diffraction and finite-aperture effects that tend to displace the waist. At the same time we imposed high and uniform transmittance, averaged over all phase-shifters. The focal length is shown to increase from 1.37 m to 1.97 m over the frequency range of interest, as desired for low-field DIII-D discharges (B = -1.57 T). Retracting the lens to receded positions rigidly moves the waists accordingly, resulting in a good match -within a fraction of the Rayleigh length- of the EC-emitting layer positions at higher fields (up to B = -2.00 T). Further, it is shown how varying the lens aperture might move the waists "non-rigidly" to better match the non-rigid movement of the EC-emitting layers with the magnetic field. The numerical method presented is very general and can be used to engineer any dependence of the focal length on the frequency, including zero or minimal chromatic aberration.Comment: 18 pages, 9 figures, http://link.springer.com/article/10.1007%2Fs10762-013-9987-

De-excitation spectroscopy of strongly interacting Rydberg gases

We present experimental results on the controlled de-excitation of Rydberg states in a cold gas of Rb atoms. The effect of the van der Waals interactions between the Rydberg atoms is clearly seen in the de-excitation spectrum and dynamics. Our observations are confirmed by numerical simulations. In particular, for off-resonant (facilitated) excitation we find that the de-excitation spectrum reflects the spatial arrangement of the atoms in the quasi one-dimensional geometry of our experiment. We discuss future applications of this technique and implications for detection and controlled dissipation schemes.Comment: 6 pages, 5 figure

Epoxy/ graphene nanocomposites – processing and properties: a review

Graphene has recently attracted significant academic and industrial interest because of its excellent performance in mechanical, electrical and thermal applications. Graphene can significantly improve physical properties of epoxy at extremely small loading when incorporated appropriately. Herein, the structure, preparation and properties of epoxy/graphene nanocomposites are reviewed in general, along with detailed examples drawn from the key scientific literature. The modification of graphene and the utilization of these materials in the fabrication of nanocomposites with different processing methods have been explored. This review has been focused on the processing methods and mechanical, electrical, thermal, and fire retardant properties of the nanocomposites. The synergic effects of graphene and other fillers in epoxy matrix have been summarised as well

Lyophilisation of influenza, rabies and Marburg lentiviral pseudotype viruses for the development and distribution of a neutralisation-assay based diagnostic kit

Pseudotype viruses (PVs) are chimeric, replication-deficient virions that mimic wild-type virus entry mechanisms and can be safely employed in neutralisation assays, bypassing the need for high biosafety requirements and performing comparably to established serological assays. However, PV supernatant necessitates -80°C long-term storage and cold-chain maintenance during transport, which limits the scope of dissemination and application throughout resource-limited laboratories. We therefore investigated the effects of lyophilisation on influenza, rabies and Marburg PV stability, with a view to developing a pseudotype virus neutralisation assay (PVNA) based kit suitable for affordable global distribution. Infectivity of each PV was calculated after lyophilisation and immediate reconstitution, as well as subsequent to incubation of freeze-dried pellets at varying temperatures, humidities and timepoints. Integrity of glycoprotein structure following treatment was also assessed by employing lyophilised PVs in downstream PVNAs. In the presence of 0.5M sucrose-PBS cryoprotectant, each freeze-dried pseudotype was stably stored for 4 weeks at up to 37°C and could be neutralised to the same potency as unlyophilised PVs when employed in PVNAs. These results confirm the viability of a freeze-dried PVNA-based kit, which could significantly facilitate low-cost serology for a wide portfolio of emerging infectious viruses

Mice with cardiac-restricted angiotensin-converting enzyme (ACE) have atrial enlargement, cardiac arrhythmia, and sudden death

Journal ArticleTo investigate the local effects of angiotensin II on the heart, we created a mouse model with 100-fold normal cardiac angiotensin-converting enzyme (ACE), but no ACE expression in kidney or vascular endothelium. This was achieved by placing the endogenous ACE gene under the control of the α-myosin heavy chain promoter using targeted homologous recombination. These mice, called ACE 8/8, have cardiac angiotensin II levels that are 4.3-fold those of wild-type mice. Despite near normal blood pressure and a normal renal function, ACE 8/8 mice have a high incidence of sudden death. Both histological analysis and in vivo catheterization of the heart showed normal ventricular size and function. In contrast, both the left and right atria were three times normal size. ECG analysis showed atrial fibrillation and cardiac block. In conclusion, increased local production of angiotensin II in the heart is not sufficient to induce ventricular hypertrophy or fibrosis. Instead, it leads to atrial morphological changes, cardiac arrhythmia, and sudden death

Targeted disruption of the even-skipped gene, evx1, causes early postimplantation lethality of the mouse conceptus.

Journal ArticleImplantation within the mammalian uterus elicits dramatic changes in the growth, differentiation, and morphogenesis of the conceptus. This process is interrupted in mice carrying a targeted disruption of the murine evx1 gene, a homolog of the Drosophila even-skipped (eve) gene. Upon implantation, presumptive evx1- homozygotes elicit a decidual response, invade the uterine epithelium, and attach to the basement membrane between uterine stroma and epithelium, but fail to differentiate extraembryonic tissues or to form egg cylinders prior to resorption. Retrograde analysis of embryo genotypes demonstrates that homozygotes could be isolated as free-floating blastocysts but not as gastrulating egg cylinders. Homozygous mutant blastocysts appeared normal and, when grown in vitro, attach, proliferate, and form trophoblastic giant cells surrounding a growing inner cell mass before rapidly degenerating. In situ hybridization analysis demonstrates evx1 gene expression within the visceral endoderm after implantation and prior to gastrulation, at a time in which the mutant phenotype is first detected

Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

Journal ArticleTwo selective procedures are compared in an effort to isolate variants of mouse L cells containing structural gene mutations. Among the resulting variant cloned cell lines are found two types of alterations in theenzyme hypoxanthine phosphoribosyl transferase (EC 2.4.2.8.) (1): enzyme with altered kinetic constants causing in vivo and in vitro resistance to 8-azaguanine; and (enzyme with altered heat sensitivity in vitro. These results support the view that tissue culture cell variantscan arise from structural gene mutations

Oxidative bio-desulfurization by nanostructured peroxidase mediator system

Bio-desulfurization is an efficient technology for removing recalcitrant sulfur derivatives from liquid fuel oil in environmentally friendly experimental conditions. In this context, the development of heterogeneous bio-nanocatalysts is of great relevance to improve the performance of the process. Here we report that lignin nanoparticles functionalized with concanavalin A are a renewable and efficient platform for the layer-by-layer immobilization of horseradish peroxidase. The novel bio-nanocatalysts were applied for the oxidation of dibenzothiophene as a well-recognized model of the recalcitrant sulfur derivative. The reactions were performed with hydrogen peroxide as a green primary oxidant in the biphasic system PBS/n-hexane at 45 °C and room pressure, the highest conversion of the substrate occurring in the presence of cationic polyelectrolyte layer and hydroxy-benzotriazole as a low molecular weight redox mediator. The catalytic activity was retained for more transformations highlighting the beneficial effect of the support in the reusability of the heterogeneous system

A gentle introduction to multiparty asynchronous session types

This article provides a gentle introduction to multiparty session types, a class of behavioural types specifically targeted at describing protocols in distributed systems based on asynchronous communication. The type system ensures well-typed processes to enjoy non-trivial properties, including communication safety, protocol fidelity, as well as progress. The adoption of multiparty session types can positively affect the whole software lifecycle, from design to deployment, improving software reliability and reducing its development costs