Additional file 2: of Does Chinese calligraphy therapy reduce neuropsychiatric symptoms: a systematic review and meta-analysis

List of studies excluded from the review by exclusion category (DOC 67 kb

Additional file 1: of Does Chinese calligraphy therapy reduce neuropsychiatric symptoms: a systematic review and meta-analysis

AF1: Search terms (DOC 30 kb

Vpr is presented in the MAM.

<p><b>A</b>, Using Percoll self-generating gradient fractionation, Vpr-GFP was localized in the ER, MAM, and mitochondria. Cyto: cytosolic fraction, LM: microsomal fraction, Mito: mitochondrial fraction. <b>B</b>, Likewise, Vpr_52–96-GFP was located in the ER, MAM, and mitochondria. <b>C</b>, Relative expression levels of Vpr-GFP and Vpr_52–96-GFP in different subcellular fractions are similar. <b>D</b>, Immunofluorescent confocal microscopy showed that Vpr was co-localized with the MAM marker, PSS-1 protein. Bulged MAM became evident in Vpr_52–96-GFP expressing cells. Yellow fluorescence (white arrow) indicates an overlap between Vpr (green fluorescence) and MAM (red fluorescence). Bar: 10 µm. <b>E</b>, Colocalization of Vpr_52–96-GFP or Vpr-GFP with the marker of ER, MAM and mitochondria was assessed based on confocal images, and 100–120 cells were scored in three independent experiments. The results showed that both proteins were present in the three organelles. <b>F,</b> HEK293 cells were transfected with the plasmid encoding HA-Vpr for 48 hours. Cells were harvested and fractionated on a self-generated Percoll gradient. HA-Vpr was localized in the ER, MAM, and mitochondria. Cyto: cytosolic fraction, LM: microsomal fraction, Mito: mitochondrial fraction. <b>G,</b> HEK293 cells were either transfected with the plasmid encoding Vpr-GFP, Vpr_52–96-GFP, or HA-Vpr for 48 hours, or infected with Lenti-Vpr (48 and 72 hours). Cells were harvested and cell cycle was analyzed by flow cytometry. Vpr-induced G2 arrest was observed in Vpr-expressing cells.</p

Knockdown of DRP1 (DRP1^KD) expression induces the accumulation of Vpr in the MAM. Ectopic expression of Vpr, on the other hand, induces nuclear translocation of DRP1.

<p><b>A</b>, Treatment with siRNA for 48 hours reduced the expression of DRP1 by approximately 80%. <b>B</b>, In DRP1^KD cells, protein levels of Vpr, Calnexin and GRP78 were increased in the MAM, while those of Vpr were decreased in the ER and mitochondria, indicating that the knockdown of DRP1 expression induced the accumulation of Vpr in the MAM. The results are representative of two independent experiments. <b>C</b>, The presence of Vpr or Vpr_52–96 increased the levels of DRP1, in particular phosphorylated DRP1 (pDRP1), in the nucleus, as determined by Western blotting. These results are representative of two independent experiments. <b>D</b>, As shown by confocal immunofluorescence microscopy, ectopic expression of Vpr_52–96 increased nuclear levels of Vpr and DRP1 (arrows with white margin). In some cells, both Vpr and DRP1 were co-localized in the nucleolus (white arrows). Bar: 10 mm. <b>E</b>, The increase of nuclear DRP1 in Vpr- or Vpr_52–96-expressing cells. Fifty cells were scored in three independent experiments with means ± S.D. * (<i>p</i><0.05) and ** (<i>p</i><0.01) indicate significantly different from the control. <b>F,</b> HA-Vpr increased nuclear levels of DRP1. <b>G</b>, and <b>H</b>, The effect of gene knockdown of Mfn2 (Mfn2^KD) or ATAD3A (ATAD3A^KD) on the organelle distribution of Vpr was determined by Western blotting. Vpr level was increased in the cytosolic fractions, but it was reduced in the mitochondrial fractions in both Mfn2^KD and ATAD3A^KD cells. Band intensities were calculated using Image J. Relative intensities are shown at the bottom of each panel.</p

Vpr downregulated Mfn2 expression via VprBP-DDB1-CUL4A ubiquitin ligase complex.

<p><b>A,</b> HEK293 cells were transfected respectively with plasmids encoding HA-Vpr and Flag-Mfn2 for 32 hours and treated with proteasome inhibitor MG132 (5 µM) for 16 hours. The expression of Mfn2 was recovered after MG132 treatment. <b>B,</b> HEK293 cells were infected with Lenti-Vpr for 56 hours and treated with proteasome inhibitor MG132 (5 µM) for 16 hours. The expression of Mfn2 was not decreased after MG132 treatment. <b>C</b>, HEK293 cells were transfected with the plasmid encoding HA-Vpr and harvested after 48 hrs. Cell lysates were immunoprecipitated with anti-HA antibody, and detected by Western blotting. <b>D,</b> HEK293 cells were transfected respectively with plasmids encoding HA-Vpr and Flag-Mfn2 for 32 hours and treated with MG132 (5 µM) for 16 hours. Cell lysates were immunoprecipitated with anti-HA antibodies, and the coimmunoprecipitated proteins were detected by Western blotting. * indicates the ubiquitinated Mfn2. <b>E,</b> HEK293 cells were silenced by shRNA against DDB1, VprBP or CUL4A, and infected with lentivirus carrying Vpr for 72 hours. The ubiquitinated Mfn2 was detected in VprBP^KD, DDB1^KD, CUL4A^KD cells. Moreover, Vpr infection did not decrease Mfn2 expression in VprBP^KD, DDB1^KD, CUL4A^KD cells. * indicates the ubiquitinated Mfn2. <b>F,</b> The MMP loss was determined by flow cytometry after Lenti-Vpr infection. Knockdown of DDB1, VprBP and CUL4A reduced the percentage of MMP loss after Lenti-Vpr infection. <b>G,</b> HEK293 cells were infected with Lenti-Vpr for 24 hours and transfected with the plasmid encoding FLAG-Mfn2 for 48 hours. Cells were harvested and analyzed by Western blotting and flow cytometry. Band intensities were calculated using Image J. Relative intensities are shown at the bottom of each panel.</p

Vpr influences the expression level of GRP78 and Mfn2 proteins.

<p><b>A</b>, At 48 hours post-transfection, expression of Vpr-GFP or Vpr_52–96-GFP up-regulated GRP78 level, but reduced Mfn2 level in HEK293 cells. <b>B</b>, Vpr-related decrease in the expression of Mfn2 was time-dependent after Lenti-Vpr infection. <b>C</b>, Vpr-related loss of mitochondrial membrane potential (MMP) was also time-dependent, indicating that the effect of Vpr on MMP change was gradual, not immediate. <b>D</b>, Infection of HEK293 cells with lentivirus carrying siRNA to Mfn2 markedly reduced protein levels 48 hours after viral treatment. <b>E</b>, Similar to that mediated by Vpr, the Mfn2 silencing-induced loss of MMP was time-dependent. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033657#s2" target="_blank">Results</a> are the means ± S.D. of three independent experiments. For panels <b>C</b> and <b>E</b>, * (<i>p</i><0.05) and *** (<i>p</i><0.001) indicate significantly different from the control. <b>F,</b> HEK293 cells were transfected with the plasmid encoding HA-Vpr for 48 hours. Cells were harvested and analyzed by Western blotting. The expression of Mfn2 was reduced in HA-Vpr expressing cells.</p

The C-terminal TMD is crucial to the integration of Vpr into MOM.

<p><b>A</b>, The C-terminal TMD is required for the subcellular distribution of Vpr to mitochondria. <b>B</b>, Using confocal immunofluorescent microscopy, Vpr-GFP was localized on the mitochondria. Mitochondria were labeled using plasmid encoding mitochondria-targeted <i>Discosoma</i> red fluorescent protein (DsRed-Mito). Yellow fluorescence indicates the overlapping regions between the Vpr and mitochondria (white arrow). Bar: 10 µm. <b>C</b>, Treatment with 100 mM sodium carbonate (pH11.5) showed that Vpr and COX IV, as integral proteins of the mitochondrial inner membrane, were resistant to sodium carbonate, while the peripherally associated matrix protein mtHsp70 was separated in the soluble fraction. All experiments were independently repeated three times. <b>D</b>, Mitochondria isolated from HA-Vpr expressing cells were treated with proteinase K. The level of HA-Vpr was decreased after proteinase K treatment, indicating that the N-terminal HA was sensitive to protease. <b>E</b>, The C-terminal GFP fragment of the Vpr-GFP was resistant to proteinase K, indicating that the C-terminus of Vpr was protected. <b>F</b>, Resistance of the C-terminal GFP fragment of Vpr_52–96-GFP to proteinase K treatment indicated that C-terminal GFP fragments were oriented toward the mitochondria. <b>G</b>, Following silencing of Tom22 or Tom40, cells were transfected with Vpr-GFP for 48 hours. Although expression of Tom22 or Tom40 was significantly reduced in gene silencing cells, expression of Vpr-GFP was not affected. <b>H</b>, The mitochondrial level of Vpr was not affected either in Tom22^KD or Tom40^KD cells as shown by Western blotting. <b>I</b>, The relative expression of mitochondrial Vpr-GFP in Tom22^KD and Tom40^KD cells was similar to that in control cells, although the mitochondrial protein COX IV was markedly reduced. The results are shown as the means ± S.D. of three independent experiments. ***The difference of relative expression is statistically significant (<i>p</i><0.001) between the control and Tom22^KD cells or that and Tom40^KD cells.</p

Transport of Vpr to the ER.

<p><b>A</b>, Confocal immunofluorescent microscopy localized Vpr at the ER. ER was labeled using plasmid encoding ER-targeted <i>Discosoma</i> red fluorescent protein (DsRed-ER). Yellow fluorescence indicates the overlapped regions between Vpr and ER (white arrow). Bar: 10 µm. <b>B</b>, Treatment with 100 mM sodium carbonate confirmed that Vpr and the ER integral protein Calnexin were present in the alkaline-resistant fraction. <b>C</b>, Isolated microsomal fractions treated with trypsin indicate that Vpr inserted into the ER by the C-terminal TMD. <b>D</b>, Using immunogold electron microscopy, Vpr particles (15 nm) were shown to be located on the membrane of mitochondria (arrowhead) and ER (arrow). These results are representative of three independent experiments.</p

Vpr-mediated mitochondrial damage causes cell death in HEK293 and CD4⁺ T cells under normal growth condition or serum starvation.

<p><b>A</b>, HEK293 cells were transfected with GFP vector the plasmid encoding Vpr-GFP or Vpr_52–96-GFP, and harvested at different time (hours) post-transfection for PI staining. The percentage of dead cells among GFP-expressing cells was determined by flow cytometry. *** (<i>p</i><0.001) indicates significantly different from the GFP vector control. <b>B</b>, HEK293 cells were infected with lenti-vector (control) or Lenti-Vpr and harvested at different time (hours) post-infection for PI staining. The percentage of dead cells was determined by flow cytometry. *** (<i>p</i><0.001) indicates significantly different from the control. <b>C,</b> HEK293 and SupT1 cells were grown in 10% FBS or starved for 24 hours, and infected with Vpr-expressing lentivirus for 48 and 72 hours. The expression of Mfn2 was decreased in serum-starved HEK293 or SupT1 cells. The quantitative expression of Mfn2 was measured by Image J and normalized with the expression of β-actin. C indicates Vpr negative lentiviral control. <b>D,</b> MMP loss was determined after Vpr-expressing lentivirus infection. Vpr significantly impaired MMP in serum-starved HEK293 and SupT1 cells. <b>E,</b> Vpr expression led to cell death in serum-starved HEK293 and SupT1 cells. For panels <b>D</b> and <b>E</b>, results are the means ± S.D. of three independent experiments. <b>*</b> (<i>p</i><0.05), ** (<i>p</i><0.01) and ** (<i>p</i><0.001) indicate significantly higher than the Vpr negative lentiviral control (Con.). † (p<0.05) and †† (<i>p</i><0.01) indicate significantly different between 10% FBS and serum starvation. <b>F,</b> Human primary CD4⁺ T cells were isolated from peripheral blood mononuclear cell (PBMC) and infected with Vpr-expressing lentivirus for 72 hours. The expression of Mfn2 was decreased and the expression of nuclear DRP1 was increased in human primary CD4⁺ cells. The relative expression levels of Mfn2 and DRP1 were measured by Image J and normalized with the expression of β-actin. <b>G,</b> MMP loss was determined after Vpr-expressing lentivirus infection. Vpr led to a significant MMP loss in human primary CD4⁺ T cells. <b>H,</b> Vpr expression led to cell death in human primary CD4⁺ T cells. For panels <b>G</b> and <b>H</b>, results are the means ± S.D. of three independent experiments. ** (<i>p</i><0.01) and *** (<i>p</i><0.001) indicate significantly higher than control human primary CD4⁺ T cells. Band intensities were calculated using Image J. Relative intensities are shown at the bottom of each panel.</p

PKCα expression and cell proliferation/migration/invasion inhibition by the truncated-MZF-1 (MZF-1ΔDBD) or-Elk-1 (Elk-1ΔDBD) proteins in SK-Hep-1 cells.

<p>The inhibitory effects of transfection with MZF-1ΔDBD and Elk-1ΔDBD constructs from RT-PCR (A) and Western blot analysis (B) assays. SK-Hep-1 cells were transiently transfected with FLAG vector (5 μg), c-Myc vector (5 μg), FLAG-MZF-1ΔDBD (5 μg), or c-Myc-Elk-1ΔDBD (5 μg). (C and D) Effect of MZF-1ΔDBD and Elk-1ΔDBD on cell growth. The SK-Hep-1 cell was transfected with the indicated dose of MZF-1ΔDBD and Elk-1ΔDBD for 1 and 2 d. Untreated cultures were designated as control (Control). Absorbance values obtained from untreated cells on day 0 after subculture were considered 100%. The migration (E and F) and invasion (G and H) assays were performed on cell cultures treated with 5 μg dose of FLAG vector, MZF-1ΔDBD, c-Myc vector, and Elk-1ΔDBD, as described in the Materials and Methods Section. Values are presented as means ± SE of three replicates from two independent experiments. **, P < 0.01 vs. FLAG or c-Myc vector-transfected group.</p