Immobilization of the Glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast

Gas1p is a glucan-elongase that plays a crucial role in yeast morphogenesis. It is predominantly anchored to the plasma membrane through a glycosylphosphatidylinositol but a fraction was also found covalently bound to the cell wall. We have used fusions with the green or red fluorescent proteins (GFP or RFP) to determine its localization. Gas1p was present in microdomains of the plasma membrane, at the mother-bud neck and in the bud scars. By exploiting the instability of RFP-Gas1p we identified mobile and immobile pools of Gas1p. Moreover, in chs3\uf044 cells the chitin ring and the cross-linked Gas1p were missing but this unveiled an additional unexpected localization of Gas1p along the septum line in cells at cytokinesis. Localization of Gas1p was also perturbed in a chs2\uf044 mutant where a remedial septum is produced. Phenotypic analysis of cells expressing a fusion of Gas1p to a trans-membrane domain unmasked new roles of the cell wall-bound Gas1p in the maintenance of the bud neck size and in cell separation. We present evidence that the Crh1p and Crh2p are required for tethering Gas1p to the chitin ring and bud scar. These results reveal a new mechanism of protein immobilization at specific sites of the cell envelop

Atomic-scale representation and statistical learning of tensorial properties

This chapter discusses the importance of incorporating three-dimensional symmetries in the context of statistical learning models geared towards the interpolation of the tensorial properties of atomic-scale structures. We focus on Gaussian process regression, and in particular on the construction of structural representations, and the associated kernel functions, that are endowed with the geometric covariance properties compatible with those of the learning targets. We summarize the general formulation of such a symmetry-adapted Gaussian process regression model, and how it can be implemented based on a scheme that generalizes the popular smooth overlap of atomic positions representation. We give examples of the performance of this framework when learning the polarizability and the ground-state electron density of a molecule

Independent measurement of the Hoyle state β\beta feeding from 12B using Gammasphere

Using an array of high-purity Compton-suppressed germanium detectors, we performed an independent measurement of the $\beta$-decay branching ratio from $^{12}\mathrm{B}$ to the second-excited (Hoyle) state in $^{12}\mathrm{C}$. Our result is $0.64(11)\%$, which is a factor $\sim 2$ smaller than the previously established literature value, but is in agreement with another recent measurement. This could indicate that the Hoyle state is more clustered than previously believed. The angular correlation of the Hoyle state $\gamma$ cascade has also been measured for the first time. It is consistent with theoretical predictions

Casimir type effects for scalar fields interacting with material slabs

We study the field theoretical model of a scalar field in presence of spacial inhomogeneities in form of one and two finite width mirrors (material slabs). The interaction of the scalar field with the defect is described with position-dependent mass term. For the single layer system we develop a rigorous calculation method and derive explicitly the propagator of the theory, S-matrix elements and the Casimir self-energy of the slab. Detailed investigation of particular limits of self-energy is presented, and connection to know cases is discussed. The calculation method is found applicable to the two mirrors case as well. By means of it we derive the corresponding Casimir energy and analyze it. For particular values of the parameters of the model the obtained results recover the Lifshitz formula. We also propose a procedure to obtain unambiguously the finite Casimir \textit{self}-energy of a single slab without reference to any renormalizations. We hope that our approach can be applied to calculation of Casimir self-energies in other demanded cases (such as dielectric ball, etc.)Comment: 22 pages, 3 figures, published version, significant changes in Section 4.

Evaluation of procalcitonin-guided antimicrobial stewardship in patients admitted to hospital with COVID-19 pneumonia

BACKGROUND: Procalcitonin is a biomarker that may be able to identify patients with COVID-19 pneumonia who do not require antimicrobials for bacterial respiratory tract co-infections. OBJECTIVES: To evaluate the safety and effectiveness of a procalcitonin-guided algorithm in rationalizing empirical antimicrobial prescriptions in non-critically ill patients with COVID-19 pneumonia. METHODS: Retrospective, single-site, cohort study in adults hospitalized with confirmed or suspected COVID-19 pneumonia and receiving empirical antimicrobials for potential bacterial respiratory tract co-infection. Regression models were used to compare the following outcomes in patients with and without procalcitonin testing within 72 h of starting antimicrobials: antimicrobial consumption (DDD); antimicrobial duration; a composite safety outcome of death, admission to HDU/ICU or readmission to hospital within 30 days; and length of admission. Procalcitonin levels of ≤0.25 ng/L were interpreted as negatively predictive of bacterial co-infection. Effects were expressed as ratios of means (ROM) or prevalence ratios (PR) accordingly. RESULTS: 259 patients were included in the final analysis. Antimicrobial use was lower in patients who had procalcitonin measured within 72 h of starting antimicrobials: mean antimicrobial duration 4.4 versus 5.4 days, adjusted ROM 0.7 (95% CI 0.6–0.9); mean antimicrobial consumption 6.8 versus 8.4 DDD, adjusted ROM 0.7 (95% CI 0.6–0.8). Both groups had similar composite safety outcomes (adjusted PR 0.9; 95% CI 0.6–1.3) and lengths of admission (adjusted ROM 1.3; 95% CI 0.9–1.6). CONCLUSIONS: A procalcitonin-guided algorithm may allow for the safe reduction of antimicrobial usage in hospitalized non-critically ill patients with COVID-19 pneumonia

A comparative study of Jet-quenching Schemes

The four major approximation schemes devised to study the modification of jets in dense matter are outlined. The comparisons are restricted to basic assumptions and approximations made in each case and the calculation methodology used. Emergent underlying similarities between apparently disparate methods brought about by the approximation schemes are exposed. Parameterizations of the medium in each scheme are discussed in terms of the transport coefficient $\hat{q}$. Discrepancies between the estimates obtained from the four schemes are discussed. Recent developments in the basic theory and phenomenology of energy loss are highlighted.Comment: 12 pages, 10 figures, latex, plenary presentation at the 19th International Conference on Ultra-Relativistic Nucleus-Nucleus Collisions (QM2006), Shanghai, China Nov. 14-20, 200

Future of the universe in modified gravitational theories: Approaching to the finite-time future singularity

We investigate the future evolution of the dark energy universe in modified gravities including $F(R)$ gravity, string-inspired scalar-Gauss-Bonnet and modified Gauss-Bonnet ones, and ideal fluid with the inhomogeneous equation of state (EoS). Modified Friedmann-Robertson-Walker (FRW) dynamics for all these theories may be presented in universal form by using the effective ideal fluid with an inhomogeneous EoS without specifying its explicit form. We construct several examples of the modified gravity which produces accelerating cosmologies ending at the finite-time future singularity of all four known types by applying the reconstruction program. Some scenarios to resolve the finite-time future singularity are presented. Among these scenarios, the most natural one is related with additional modification of the gravitational action in the early universe. In addition, late-time cosmology in the non-minimal Maxwell-Einstein theory is considered. We investigate the forms of the non-minimal gravitational coupling which generates the finite-time future singularities and the general conditions for this coupling in order that the finite-time future singularities cannot emerge. Furthermore, it is shown that the non-minimal gravitational coupling can remove the finite-time future singularities or make the singularity stronger (or weaker) in modified gravity.Comment: 25 pages, no figure, title changed, accepted in JCA

Gamow Shell Model Description of Weakly Bound Nuclei and Unbound Nuclear States

We present the study of weakly bound, neutron-rich nuclei using the nuclear shell model employing the complex Berggren ensemble representing the bound single-particle states, unbound Gamow states, and the non-resonant continuum. In the proposed Gamow Shell Model, the Hamiltonian consists of a one-body finite depth (Woods-Saxon) potential and a residual two-body interaction. We discuss the basic ingredients of the Gamow Shell Model. The formalism is illustrated by calculations involving {\it several} valence neutrons outside the double-magic core: $^{6-10}$He and $^{18-22}$O.Comment: 19 pages, 20 encapsulated PostScript figure

Fecal Microbiota Transplantation in Gestating Sows and Neonatal Offspring Alters Lifetime Intestinal Microbiota and Growth in Offspring

Previous studies suggest a link between intestinal microbiota and porcine feed efficiency (FE). Therefore, we investigated whether fecal microbiota transplantation (FMT) in sows and/or neonatal offspring, using inocula derived from highly feed-efficient pigs, could improve offspring FE. Pregnant sows were assigned to control or FMT treatments and the subsequent offspring to control treatment, FMT once (at birth), or FMT four times (between birth and weaning). FMT altered sow fecal and colostrum microbiota compositions and resulted in lighter offspring body weight at 70 and 155 days of age when administered to sows and/or offspring. This was accompanied by FMT-associated changes within the offspring?s intestinal microbiota, mostly in the ileum. These included transiently higher fecal bacterial diversity and load and numerous compositional differences at the phylum and genus levels (e.g., Spirochaetes and Bacteroidetes at high relative abundances and mostly members of Clostridia, respectively), as well as differences in the abundances of predicted bacterial pathways. In addition, intestinal morphology was negatively impacted, duodenal gene expression altered, and serum protein and cholesterol concentrations reduced due to FMT in sows and/or offspring. Taken together, the results suggest poorer absorptive capacity and intestinal health, most likely explaining the reduced body weight. An additive effect of FMT in sows and offspring also occurred for some parameters. Although these findings have negative implications for the practical use of the FMT regime used here for improving FE in pigs, they nonetheless demonstrate the enormous impact of early-life intestinal microbiota on the host phenotype.publishersversionPeer reviewe

Manganites at Quarter Filling: Role of Jahn-Teller Interactions

We have analyzed different correlation functions in a realistic spin-orbital model for half-doped manganites. Using a finite-temperature diagonalization technique the CE phase was found in the charge-ordered phase in the case of small antiferromagnetic interactions between $t_{2g}$ electrons. It is shown that a key ingredient responsible for stabilization of the CE-type spin and orbital-ordered state is the cooperative Jahn-Teller (JT) interaction between next-nearest Mn$^{+3}$ neighbors mediated by the breathing mode distortion of Mn$^{+4}$ octahedra and displacements of Mn$^{+4}$ ions. The topological phase factor in the Mn-Mn hopping leading to gap formation in one-dimensional models for the CE phase as well as the nearest neighbor JT coupling are not able to produce the zigzag chains typical for the CE phase in our model.Comment: 16 pages with 16 figures, contains a more detailed parameter estimate based on the structural data by Radaelli et al. (accepted for publication in Phys. Rev. B