OPTIMIZATION OF LIPASE PRODUCTION FROM DIFFERENT AGROINDUSTRIAL WASTES BY MARINE ACTINOMYCETES

Objective: To identify the production of extracellular lipase by solid state fermentation (SSF) using coconut cake and groundnut cake using thermophilic strain of marine actinomycetes. Methods: From the isolated strains of marine actinomycetes a comparative study has been evaluated on different intends such as pH, temperature, carbon sources, nitrogen sources and additive sources were optimized for maximum yield. Results: Five different pH was used from 5 to 9 and on the other hand 3 different temperatures were maintained, sucrose, fructose and dextrose were used as carbon sources while urea, peptone and Ammonium nitrate was used as nitrogen sources similarly Tween 20 was used as additive sources. On comparing coconut oil cake and groundnut oil cake for all these different intends, there was maximum extracellular lipase specific activity was obtained in groundnut oil cake