FIGURE 2 in Supplementary characters of Bursaphelenchus lini Braasch, 2004 (Nematoda: Parasitaphelenchidae) and remarks on this nematode

FIGURE 2. ITS­RFLP patterns obtained from two individual specimens of Bursaphelenchus lini (left: type A, right: type B). Restriction fragments were obtained by digestion of the amplified rDNA fragment (0) with Rsa I (1), Hae III (2), Msp I (3), Hinf I (4) and Alu I (5). M: DNA size marker (100 bp ladder; Invitrogen Life Technologies).Published as part of <i>Braasch, Helen, Brandstetter, Martin & Burgermeister, Wolfgang, 2006, Supplementary characters of Bursaphelenchus lini Braasch, 2004 (Nematoda: Parasitaphelenchidae) and remarks on this nematode, pp. 55-61 in Zootaxa 1141 (1)</i> on page 60, DOI: 10.11646/zootaxa.1141.1.2, <a href="http://zenodo.org/record/10087138">http://zenodo.org/record/10087138</a&gt

Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses

Random Amplified Polymorphic DNA (RAPD-PCR) technique was used in order to assess the level of genetic variability and genetic relationships among 24 Portuguese isolates of pinewood nematode, Bursaphelenchus xylophilus, representative of the fully affected area in Portugal. Two additional isolates of B. xylophilus, representing North America and East Asia were included, and B. mucronatus was used as out-group. Twenty-eight random primers generated a total of 640 DNA fragments. The Nei & Li similarity index revealed a high genetic similarity among the Portuguese isolates (above 90%). Hierarchical cluster analysis was performed to illustrate the relatedness among the isolates. No indication for separate groups among the Portuguese isolates was obtained, and the low level of genetic diversity strongly suggests that they were dispersed recently from a single introduction. The lack of apparent relationship between the genetic and the geographic matrices of the Portuguese isolates limits the use of this technique for following recent pathways of distribution. Genetic distance of the Portuguese isolates towards an isolate from China was much lower as compared to an isolate from the USA. This confirmed previous results suggesting an East Asian origin of the Portuguese B. xylophilus

FIGURE 1 in Supplementary characters of Bursaphelenchus lini Braasch, 2004 (Nematoda: Parasitaphelenchidae) and remarks on this nematode

FIGURE 1. Scanning electron micrographs of Bursaphelenchus lini: A. Head region, lateral view; B. Enface view of female head; C. Lateral field; D. Female tail; E. Vulval region; F.­I: Male tail showing papillae (F, G), bursa (H) and protracted spicules (I).Published as part of <i>Braasch, Helen, Brandstetter, Martin & Burgermeister, Wolfgang, 2006, Supplementary characters of Bursaphelenchus lini Braasch, 2004 (Nematoda: Parasitaphelenchidae) and remarks on this nematode, pp. 55-61 in Zootaxa 1141 (1)</i> on page 59, DOI: 10.11646/zootaxa.1141.1.2, <a href="http://zenodo.org/record/10087138">http://zenodo.org/record/10087138</a&gt

FIGURE 4 in Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group

FIGURE 4. ITS­RFLP patterns of Bursaphelenchus singaporensis sp. n. and six morphologically similar species. Each picture shows (from left to right) DNA marker (100 bp ladder, Invitrogen), rDNA amplification product, restriction fragments obtained with Rsa I, Hae III, Msp I, Hinf I, Alu I, and again DNA marker.Published as part of <i>GU, JIANFENG, ZHANG, JIANCHENG, BRAASCH, HELEN & BURGERMEISTER, WOLFGANG, 2005, Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group, pp. 1-12 in Zootaxa 988 (1)</i> on page 9, DOI: 10.11646/zootaxa.988.1.1, <a href="http://zenodo.org/record/10087012">http://zenodo.org/record/10087012</a&gt

FIGURE 1 in Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group

FIGURE 1. Bursaphelenchus singaporensis sp. n. A: Female; B: Male; C: Head region; D: Female tails; E: Male tail; F: Vulval region; G: Bursa; H:. Spicules. (Scale bars = 10 µm).Published as part of <i>GU, JIANFENG, ZHANG, JIANCHENG, BRAASCH, HELEN & BURGERMEISTER, WOLFGANG, 2005, Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group, pp. 1-12 in Zootaxa 988 (1)</i> on page 5, DOI: 10.11646/zootaxa.988.1.1, <a href="http://zenodo.org/record/10087012">http://zenodo.org/record/10087012</a&gt

FIGURE 2 in Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group

FIGURE 2. Light photomicrographs of Bursaphelenchus singaporensis sp. n. A, B: Head region; C, D: Female tail; E, F: Male bursa; G, H: Male tail; I: Vulval region. (Scale bars = 10 µm).Published as part of <i>GU, JIANFENG, ZHANG, JIANCHENG, BRAASCH, HELEN & BURGERMEISTER, WOLFGANG, 2005, Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group, pp. 1-12 in Zootaxa 988 (1)</i> on page 6, DOI: 10.11646/zootaxa.988.1.1, <a href="http://zenodo.org/record/10087012">http://zenodo.org/record/10087012</a&gt