351 research outputs found
Identifying the source of unknown microcystin genes and predicting microcystin variants by comparing genes within uncultured cyanobacterial cells
While multiple phylogenetic markers have been used in the culture independent study of microcystin producing cyanobacteria, in only a few instances have multiple markers been studied within individual cells, and in all cases these studies have been conducted with cultured isolates. Here, we isolate and evaluate large DNA fragments (\u3e 6 kb) encompassing two genes involved in microcystin biosynthesis (mcyA2 and mcyB1) and use them to identify the source of gene fragments found in water samples. Further investigation of these gene loci from individual cyanobacterial cells allowed for improved analysis of the genetic diversity within microcystin producers as well as a method to predict microcystin variants for individuals. These efforts have also identified the source of the novel mcyA genotype previously termed Microcystis-like that is pervasive in the Laurentian Great Lakes and predict the microcystin variant(s) that it produces
Effects of Noisy Oracle on Search Algorithm Complexity
Grover's algorithm provides a quadratic speed-up over classical algorithms
for unstructured database or library searches. This paper examines the
robustness of Grover's search algorithm to a random phase error in the oracle
and analyzes the complexity of the search process as a function of the scaling
of the oracle error with database or library size. Both the discrete- and
continuous-time implementations of the search algorithm are investigated. It is
shown that unless the oracle phase error scales as O(N^(-1/4)), neither the
discrete- nor the continuous-time implementation of Grover's algorithm is
scalably robust to this error in the absence of error correction.Comment: 16 pages, 4 figures, submitted to Phys. Rev.
Normal and Lateral Casimir Forces between Deformed Plates
The Casimir force between macroscopic bodies depends strongly on their shape
and orientation. To study this geometry dependence in the case of two deformed
metal plates, we use a path integral quantization of the electromagnetic field
which properly treats the many-body nature of the interaction, going beyond the
commonly used pairwise summation (PWS) of van der Waals forces. For arbitrary
deformations we provide an analytical result for the deformation induced change
in Casimir energy, which is exact to second order in the deformation amplitude.
For the specific case of sinusoidally corrugated plates, we calculate both the
normal and the lateral Casimir forces. The deformation induced change in the
Casimir interaction of a flat and a corrugated plate shows an interesting
crossover as a function of the ratio of the mean platedistance H to the
corrugation length \lambda: For \lambda \ll H we find a slower decay \sim
H^{-4}, compared to the H^{-5} behavior predicted by PWS which we show to be
valid only for \lambda \gg H. The amplitude of the lateral force between two
corrugated plates which are out of registry is shown to have a maximum at an
optimal wavelength of \lambda \approx 2.5 H. With increasing H/\lambda \gtrsim
0.3 the PWS approach becomes a progressively worse description of the lateral
force due to many-body effects. These results may be of relevance for the
design and operation of novel microelectromechanical systems (MEMS) and other
nanoscale devices.Comment: 20 pages, 5 figure
Seasonal Gene Expression and the Ecophysiological Implications of Toxic Microcystis aeruginosa Blooms in Lake Taihu
Harmful cyanobacterial blooms represent an increasing threat to freshwater resources globally. Despite increased research, the physiological basis of how the dominant bloom-forming cyanobacteria, Microcystis spp., proliferate and then maintain high population densities through changing environmental conditions is poorly understood. In this study, we examined the transcriptional profiles of the microbial community in Lake Taihu, China at 9 stations sampled monthly from June to October in 2014. To target Microcystis populations, we collected metatranscriptomic data and mapped reads to the M. aeruginosa NIES 843 genome. Our results revealed significant temporal gene expression patterns, with many genes separating into either early or late bloom clusters. About one-third of genes observed from M. aeruginosa were differentially expressed between these two clusters. Conductivity and nutrient availability appeared to be the environmental factors most strongly associated with these temporal gene expression shifts. Compared with the early bloom season (June and July), genes involved in N and P transport, energy metabolism, translation, and amino acid biosynthesis were down-regulated during the later season (August to October). In parallel, genes involved in regulatory functions as well as transposases and the production of microcystin and extracellular polysaccharides were up-regulated in the later season. Our observation indicates an eco-physiological shift occurs within the Microcystis spp. transcriptome as cells move from the rapid growth of early summer to bloom maintenance in late summer and autumn
The Casimir force and the quantum theory of lossy optical cavities
We present a new derivation of the Casimir force between two parallel plane
mirrors at zero temperature. The two mirrors and the cavity they enclose are
treated as quantum optical networks. They are in general lossy and
characterized by frequency dependent reflection amplitudes. The additional
fluctuations accompanying losses are deduced from expressions of the optical
theorem. A general proof is given for the theorem relating the spectral density
inside the cavity to the reflection amplitudes seen by the inner fields. This
density determines the vacuum radiation pressure and, therefore, the Casimir
force. The force is obtained as an integral over the real frequencies,
including the contribution of evanescent waves besides that of ordinary waves,
and, then, as an integral over imaginary frequencies. The demonstration relies
only on general properties obeyed by real mirrors which also enforce general
constraints for the variation of the Casimir force.Comment: 18 pages, 6 figures, minor amendment
Roles of Nutrient Limitation on Western Lake Erie CyanoHAB Toxin Production
Cyanobacterial harmful algal bloom (CyanoHAB) proliferation is a global problem impacting ecosystem and human health. Western Lake Erie (WLE) typically endures two highly toxic CyanoHABs during summer: a Microcystis spp. bloom in Maumee Bay that extends throughout the western basin, and a Planktothrix spp. bloom in Sandusky Bay. Recently, the USA and Canada agreed to a 40% phosphorus (P) load reduction to lessen the severity of the WLE blooms. To investigate phosphorus and nitrogen (N) limitation of biomass and toxin production in WLE CyanoHABs, we conducted in situ nutrient addition and 40% dilution microcosm bioassays in June and August 2019. During the June Sandusky Bay bloom, biomass production as well as hepatotoxic microcystin and neurotoxic anatoxin production were N and P co-limited with microcystin production becoming nutrient deplete under 40% dilution. During August, the Maumee Bay bloom produced microcystin under nutrient repletion with slight induced P limitation under 40% dilution, and the Sandusky Bay bloom produced anatoxin under N limitation in both dilution treatments. The results demonstrate the importance of nutrient limitation effects on microcystin and anatoxin production. To properly combat cyanotoxin and cyanobacterial biomass production in WLE, both N and P reduction efforts should be implemented in its watershed
Local and Global Casimir Energies: Divergences, Renormalization, and the Coupling to Gravity
From the beginning of the subject, calculations of quantum vacuum energies or
Casimir energies have been plagued with two types of divergences: The total
energy, which may be thought of as some sort of regularization of the
zero-point energy, , seems manifestly divergent. And
local energy densities, obtained from the vacuum expectation value of the
energy-momentum tensor, , typically diverge near
boundaries. The energy of interaction between distinct rigid bodies of whatever
type is finite, corresponding to observable forces and torques between the
bodies, which can be unambiguously calculated. The self-energy of a body is
less well-defined, and suffers divergences which may or may not be removable.
Some examples where a unique total self-stress may be evaluated include the
perfectly conducting spherical shell first considered by Boyer, a perfectly
conducting cylindrical shell, and dilute dielectric balls and cylinders. In
these cases the finite part is unique, yet there are divergent contributions
which may be subsumed in some sort of renormalization of physical parameters.
The divergences that occur in the local energy-momentum tensor near surfaces
are distinct from the divergences in the total energy, which are often
associated with energy located exactly on the surfaces. However, the local
energy-momentum tensor couples to gravity, so what is the significance of
infinite quantities here? For the classic situation of parallel plates there
are indications that the divergences in the local energy density are consistent
with divergences in Einstein's equations; correspondingly, it has been shown
that divergences in the total Casimir energy serve to precisely renormalize the
masses of the plates, in accordance with the equivalence principle.Comment: 53 pages, 1 figure, invited review paper to Lecture Notes in Physics
volume in Casimir physics edited by Diego Dalvit, Peter Milonni, David
Roberts, and Felipe da Ros
The state of the Martian climate
60°N was +2.0°C, relative to the 1981–2010 average value (Fig. 5.1). This marks a new high for the record. The average annual surface air temperature (SAT) anomaly for 2016 for land stations north of starting in 1900, and is a significant increase over the previous highest value of +1.2°C, which was observed in 2007, 2011, and 2015. Average global annual temperatures also showed record values in 2015 and 2016. Currently, the Arctic is warming at more than twice the rate of lower latitudes
Anisotropic flow of charged hadrons, pions and (anti-)protons measured at high transverse momentum in Pb-Pb collisions at TeV
The elliptic, , triangular, , and quadrangular, , azimuthal
anisotropic flow coefficients are measured for unidentified charged particles,
pions and (anti-)protons in Pb-Pb collisions at TeV
with the ALICE detector at the Large Hadron Collider. Results obtained with the
event plane and four-particle cumulant methods are reported for the
pseudo-rapidity range at different collision centralities and as a
function of transverse momentum, , out to GeV/.
The observed non-zero elliptic and triangular flow depends only weakly on
transverse momentum for GeV/. The small dependence
of the difference between elliptic flow results obtained from the event plane
and four-particle cumulant methods suggests a common origin of flow
fluctuations up to GeV/. The magnitude of the (anti-)proton
elliptic and triangular flow is larger than that of pions out to at least
GeV/ indicating that the particle type dependence persists out
to high .Comment: 16 pages, 5 captioned figures, authors from page 11, published
version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/186
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