Evaluation of Gingival Crevicular Fluid Cortisol Levels with HPLC Method

Objectives: The main aim of the present study was to evaluate the cortisol levels in gingival crevicular fluid by high performance liquid chromatography method. Secondary aim was to compare the cortisol levels in several periodontal diseases

Periodontal disease and systemic diseases in an older population

WOS: 000341018300039PubMed ID: 25015876Objective: To evaluate the relationship between older adults' medical and oral conditions and their self-reports of periodontal conditions with clinically obtained data. Background: Concerns about oral health of elders and its association with systemic diseases have been gaining more attention. Methods: A total of 201 older subjects were interviewed about their previous medical and dental histories and were asked to complete a health questionnaire. Each subject received full mouth exam, including counting number of natural teeth remaining, gingival (GI) and plaque index (PI), CPITN and denture status. Results: Elders who completed health questionnaires had mean age of 62.5. Mean CPITN score was 1.62(+/- 1.12), PI was 1.57(+/- 1.48), and GI was 1.55(+/- 1.31). Women had higher prevalence of CVD and osteoporosis than men (p = 0.008, p = 0.0001, respectively). Subjects who reported bleeding upon brushing had higher PI and GI scores (p = 0.03, p = 0.05, respectively). Smokers were more likely to describe their periodontal tissues as unhealthy (72.3% vs. 27.7%, p = 0.01), whereas self-reports of healthy vs. unhealthy gums did not differ between non-smokers. Conclusion: These findings suggest that a number of systemic conditions are associated with indicators of periodontal disease, and self-reports of oral conditions are independent of systemic diseases. (C) 2014 Elsevier Ireland Ltd. All rights reserved

Effects of potassium aluminum sulfate on TNF-alpha, MMP-1 and MMP-8 levels at gingival crevicular fluid in periodontally healthy subjects: a pilot study

WOS: 000314288600012Objectives: The aim of this study was to investigate the possible effects of fixed dental prosthesis procedures with and without gingival retraction containing potassium aluminum sulfate on TNF-alpha, MMP -1 and MMP-8 levels at gingival crevicular fluid in periodontally healthy subjects and to demonstrate that these markers can be used for the assessment of disease activity. Methods: Eight healthy subjects, each with at least two abutment teeth, were included in our research. Gingival crevicular fluid (GCF) samples were obtained before initiation of fixed dental prosthesis treatment and repeated on days 2, 3, 7 and first month after cementation. GCF concentrations of MMP-1, MMP-8 and TNF-alpha were determined by ELISA. ANOVA was used for statistical analysis. Results: The GCF MMP-8 concentrations revealed significant differences among study groups (p0.05). The MMP-8 concentrations of control group was significantly decreased in the first month (p<0.05). The TNF-alpha concentrations showed significant differences in all study groups (p<0.05). In contrast to ret(-) and control groups, TNF-alpha concentrations of ret(+) group was significantly different among sampling days (p<0.05). The TNF-alpha concentration peaked in the first month in ret(+) group. Conclusion: Within the scope of this study, it may be suggested that well-fitted fixed dental prosthesis and its procedures performed to periodontally healthy subjects do not affect the periodontal tissue health negatively.Ege University Scientific Research Support CommitteeEge University [018-DIS-2008]This study was solely supported by the Ege University Scientific Research Support Committee (018-DIS-2008)

Plasma Levels of C-Telopeptide Pyridinoline Cross-Links of Type I Collagen and Osteocalcin in Chronic Periodontitis

WOS: 000290234500007PubMed ID: 20577791This study was planned to investigate whether chronic periodontitis patients exhibit different plasma concentrations of C-telopeptide pyridinoline cross-links of type I collagen (ICTP) and osteocalcin (OC) compared to the clinically healthy controls. Before initiation of any periodontal intervention, plasma samples and full-mouth clinical periodontal recordings were obtained from 58 otherwise healthy patients with chronic periodontitis and also from 47 systemically and periodontally healthy control subjects. Plasma ICTP levels were determined by radioimmunoassay and OC levels by enzyme-linked immunosorbent assay. Data were tested statistically using t test and ANOVA. The healthy control group exhibited significantly lower values in all clinical periodontal measurements (P 0.05). Within the limits of this study, it may be suggested that plasma levels of ICTP and OC may not provide distinguishing data between chronic periodontitis patients and clinically healthy subjects

Is There an Interaction Between Polycystic Ovary Syndrome and Gingival Inflammation?

WOS: 000312355600012PubMed ID: 22509751Background: The aim of this study is to evaluate the gingival crevicular fluid (GCF), saliva, and serum concentrations of tumor necrosis factor-alpha (TNF-alpha), TNF-alpha receptor-1 (TNF-alpha R1), TNF-alpha R2, and interleukin-6 (IL-6) in non-obese females with polycystic ovary syndrome (PCOS) and either clinically healthy periodontium or gingivitis. Methods: Thirty-one females with PCOS and healthy periodontium, 30 females with PCOS and gingivitis, and 12 systemically and periodontally healthy females were included in the study. GCF, saliva, and serum samples were collected, and clinical periodontal measurements, body mass index, and Ferriman-Gallwey score (FGS) were recorded. Sex hormones, cortisol, and insulin levels were measured. TNF-alpha, THF-alpha 1R1, THF-alpha R2, and IL-6 were determined by enzyme-linked immunosorbent assay. Kruskal-Wallis followed by Bonferroni-corrected post hoc Mann-Whitney U tests were used to analyze the data. Results: The PCOS + gingivitis group revealed significantly higher GCF, saliva, and serum IL-6 concentrations than the PCOS + healthy group (P < 0.0001). The two PCOS groups exhibited significantly higher saliva TNF-alpha concentrations than the control group (P = 0.024 and P = 0.013, respectively). The FGS index was significantly higher in the PCOS + gingivitis group than the PCOS + healthy group (P = 0.030). The PCOS + gingivitis group revealed significantly higher insulin concentration than the PCOS + healthy and control groups (P = 0.014 and P < 0.0001, respectively). Serum TNF-alpha, TNF-alpha Rs, and serum, GCF, and salivary IL-6 levels correlated with the clinical periodontal measurements. Conclusions: PCOS and gingival inflammation appear to act synergistically on the proinflammatory cytokines IL-6 and TNF-alpha. Thus, PCOS may have an impact on gingival inflammation or vice versa. Additional studies are warranted to clarify the possible relationship between PCOS and periodontal disease. J Periodontol 2012;83:1529-1537.Ege University Research FoundationEge University [2009 DIS 024]This study was supported by Ege University Research Foundation Grant 2009 DIS 024. The authors report no conflicts of interest related to this study