Mechanisms for the maintenance and eventual degradation of neurofilament proteins in the distal segments of severed goldfish Mauthner axons

Cellular mechanisms that might affect the degradation of neurofilament proteins (NFPs) were examined in the distal segments of severed goldfish Mauthner axons (M-axons), which do not degenerate for more than 2 months after severance. Calpain levels, as determined by reactivity to a polyclonal antibody, remained constant for 80 d postseverance in distal segments of M-axons and then declined from 80 to 85 d postseverance. Calpain activity in rat brain, as determined by a spectrophotometric assay, was much higher than calpain activity in control and severed goldfish brain, spinal cord, muscle, or M-axons. Calpain activity was extremely low in M-axons compared with that in all other tissues and remained low for up to 80 d postseverance in distal segments of M-axons. Phosphorylated NFPs, as determined by Stains-All treatment of SDS gels, were maintained for up to 72 d postseverance and then decreased noticeably at 75 d postseverance when NFP breakdown products appeared on silver-stained gels. By 85 d postseverance, phosphorylated NFPs no longer were detected, and NFP breakdown products were the most prominent bands on silver-stained gels. These results suggest that the distal segments of M-axons survive for months after severance, because NFPs are maintained in a phosphorylated state that stabilizes and protects NFPs from degradation by low levels of calpain activity in the M-axon; the distal segments of severed M-axons degenerate eventually when NFPs no longer are maintained in a phosphorylated state and become susceptible to degradation, possibly by low levels of calpain activity in the M-axon.This work was supported by an Advanced Technology Project Grant to G.D.B.Neuroscienc

Phase behavior of a fluid with competing attractive and repulsive interactions

Fluids in which the interparticle potential has a hard core, is attractive at moderate separations, and repulsive at greater separations are known to exhibit novel phase behavior, including stable inhomogeneous phases. Here we report a joint simulation and theoretical study of such a fluid, focusing on the relationship between the liquid-vapor transition line and any new phases. The phase diagram is studied as a function of the amplitude of the attraction for a certain fixed amplitude of the long ranged repulsion. We find that the effect of the repulsion is to substitute the liquid-vapor critical point and a portion of the associated liquid-vapor transition line, by two first order transitions. One of these transitions separates the vapor from a fluid of spherical liquidlike clusters; the other separates the liquid from a fluid of spherical voids. At low temperature, the two transition lines intersect one another and a vapor-liquid transition line at a triple point. While most integral equation theories are unable to describe the new phase transitions, the Percus Yevick approximation does succeed in capturing the vapor-cluster transition, as well as aspects of the structure of the cluster fluid, in reasonable agreement with the simulation results.Comment: 15 pages, 20 figure

Methane hydrate stability and anthropogenic climate change

International audienceMethane frozen into hydrate makes up a large reservoir of potentially volatile carbon below the sea floor and associated with permafrost soils. This reservoir intuitively seems precarious, because hydrate ice floats in water, and melts at Earth surface conditions. The hydrate reservoir is so large that if 10% of the methane were released to the atmosphere within a few years, it would have an impact on the Earth's radiation budget equivalent to a factor of 10 increase in atmospheric CO2. Hydrates are releasing methane to the atmosphere today in response to anthropogenic warming, for example along the Arctic coastline of Siberia. However most of the hydrates are located at depths in soils and ocean sediments where anthropogenic warming and any possible methane release will take place over time scales of millennia. Individual catastrophic releases like landslides and pockmark explosions are too small to reach a sizable fraction of the hydrates. The carbon isotopic excursion at the end of the Paleocene has been interpreted as the release of thousands of Gton C, possibly from hydrates, but the time scale of the release appears to have been thousands of years, chronic rather than catastrophic. The potential climate impact in the coming century from hydrate methane release is speculative but could be comparable to climate feedbacks from the terrestrial biosphere and from peat, significant but not catastrophic. On geologic timescales, it is conceivable that hydrates could release much carbon to the atmosphere/ocean system as we do by fossil fuel combustion

Scale effects on the hydrological impact of upland afforestation and drainage using indices of flow variability: the River Irthing, England

International audienceFrequent assertions by river users that rivers in northern England now rise and fall more quickly than in the past, have never been validated by analysis on catchments of more than 10 km2. The method using indices of flow variability provides a basis for making direct measurements of the annual number and duration of pulses, i.e. rises above a given flow, and for comparing catchments of different sizes. A comparison is made between the small afforested headwater Coalburn catchment (1.5 km2) and the larger River Irthing catchment (335 km2) on which the afforested area comprises 19%. A simple but effective means is provided for decoupling the effect of climatic variability from the effects of land use. The analysis shows that major changes have occurred on the small catchment, first with rising pulse numbers after pre-afforestation drainage, then with a much greater progressive decrease in pulse number accompanied with increasing pulse duration. In contrast, the larger catchment shows little change in variability indices from the beginning of its record in 1968 until the late 1980s after which the pattern of change mirrors that at Coalburn but the proportional change is much smaller. The direction of change is the opposite of that asserted by river users. Keywords: hydrology, flow variability, land-use impacts, forests, scale effect

Change in Working Length at Different Stages of Instrumentation as a Function of Canal Curvature

The aim of this study was to determine the change in working length (∆WL) before and after coronal flaring and after complete rotary instrumentation as a function of canal curvature. One mesiobuccal or mesiolingual canal from each of 43 extracted molars had coronal standardization and access performed. Once the access was completed, canal preparation was accomplished using Gates Glidden drills for coronal flaring and EndoSequence files for rotary instrumentation. WLs were obtained at 3 time points: pre-instrumentation (unflared), mid-instrumentation (flared) and post-instrumentation (concluded). Measurements were made via direct visualization (DV) and the CanalPro apex locator (EM) in triplicate by a single operator with blinding within the time points. Root curvature was measured using Schneider’s technique. The change in working length was assessed using repeated-measures ANCOVA. The direct visualization measurements were statistically larger than the electronic measurements (paired t-test difference = 0.20 mm, SE = 0.037, P \u3c .0001), although a difference this large may not be clinically important. Overall, a greater change in working length was observed in straight canals than in curved canals, and this trend was more pronounced when measured electronically than via direct visualization, especially in the unflared-concluded time points compared with unflared-flared time points. A greater change in working length was also observed in longer canals than in shorter canals.https://scholarscompass.vcu.edu/gradposters/1032/thumbnail.jp

An Investigation of the Relationships Between the Angle of Mental Rotation Required For Spatial Orientation, Response Times, and Accuracy

The purpose of this study is to investigate the relationship between the angles of mental rotation when attempting to spatially orientate and the resulting response times and levels of accuracy. By means of a computer program, participants were presented with 64 mental rotational trials. The mental rotational trials consisted of a triangle placed in the center of the screen with a standard stick symbol of an aircraft appearing at various headings and orientations around the triangle. The participants were required to imagine themselves inside the flight deck of the aircraft, and then respond as quickly and accurately as possible to where the triangle is in relation to their orientation. Analysis of the data indicated that as the amount of angular displacement increased from the straight ahead and directly behind positions, the response times and accuracy rates increased and decreased respectively. Additionally, responses for the cardinal orientations were faster than the non-cardinal orientations

Development of methodologies and procedures for identifying STS users and uses

A study was conducted to identify new uses and users of the new Space Transporation System (STS) within the domestic government sector. The study develops a series of analytical techniques and well-defined functions structured as an integrated planning process to assure efficient and meaningful use of the STS. The purpose of the study is to provide NASA with the following functions: (1) to realize efficient and economic use of the STS and other NASA capabilities, (2) to identify new users and uses of the STS, (3) to contribute to organized planning activities for both current and future programs, and (4) to air in analyzing uses of NASA's overall capabilities