Design of a Seat Mounting with a Rail Vehicle Frame

V předkládané diplomové práci, kterou jsem vypracoval ve spolupráci s VŠB – TU Ostrava a společností Siemens s.r.o., se věnuji návrhu upevnění sedadel k boční stěně hrubé stavby kolejového vozidla. Práce souvisí s projektem výstavby metra ve městě Riyadh. Hlavními částmi této práce jsou variantní návrh upevnění pomocí tzv. cantileveru, rozpracování zvoleného řešení, pevnostně deformační analýza a kontrola šroubového spoje. Součástí práce je také teoretický rozbor norem a vyhlášek souvisejících s návrhem sedadlových systémů v kolejových vozidlech.In the presented master´s thesis, which I developed in cooperation with VŠB – TU Ostrava and company Siemens s.r.o., I designed variant Fastening of a Seats to the side wall of Rail Vehicle Frame. Thesis related with project Metro Riyadh. The main parts of this thesis are variant design of cantilever, Fastening of a seats, stress – strain analysis and check of bolted connection. Creation of drawing documentation is another part.347 - Katedra částí a mechanismů strojůvýborn

Ensemble cell-wide kinetic modeling of anaerobic organisms to support fuels and chemicals production

Thermophilic microorganisms have garnered the interest of the bioprocess industry due to their high temperature optimal growth conditions. In particular, two phylogenetically close organisms Clostridium thermocellum and Moorella thermoacetica have been focused on in the recent years. While C. thermocellum can metabolize cellulose into biofuels such as ethanol, M. thermoacetica can metabolize syn gas using the unique Wood-Ljungdahl pathway. Despite their increasing role as bio-production platforms, they remain poorly characterized with significant uncertainty in their metabolic repertoire. To this end, we develop cell-wide dynamic models of transcription and metabolism of these two organisms using the concept of Ensemble Modeling (EM) which requires curated genome-scale metabolic (GSM) models of the organisms as its foundation. The second generation GSM for C. thermocellum (iCth446) has been developed, which contains 446 genes and includes 598 metabolites and 637 reactions, along with gene-protein-reaction associations. The GSM is devoid of thermodynamically infeasible cycles and contains elementally and charge-balanced reactions. The GSM was simulated with down-regulation of phosphoenolpyruvate carboxykinase, or down-regulation of malic enzyme and malate dehydrogenase knocked out along with exogenous pyruvate kinase knocked in and lactate dehydrogenase knocked out. The simulations showed higher yield of ethanol production compared to wild-type conditions as observed experimentally. Likewise, the GSM results showed that only lactate hydrogenase knock out did not have any effect on growth rate as observed experimentally. The GSM model for M. thermoacetica has been constructed using a semi-automatic pipeline in the SEED database. This model contains 517 genes and 812 reactions, supplemented with 57 exchange reactions including biomass demand reaction. The GSM will be curated, gap-filled, and incorporated with the estimated growth and non-growth associated ATP (GAM & NGAM) requirements. Steady-state 13C-labelling experiments will be used to validate flux distribution predicted by the GSM, followed by instationary 13Clabeling experiments to identify the robustness of the GSM under various growth conditions with different substrates in distinct growth phases. Ultimately, these experiments will resolve the long-standing debate surrounding the hypothetical incomplete TCA cycle of M. thermoacetica. The constructed stoichiometry representations will subsequently serve as the scaffold for building kinetic model using the EM approach for C. thermocellum and M. thermoacetica. The EM procedure will allow us to integrate substrate-level as well as transcriptional level regulatory interactions into the framework. The constructed kinetic models will be ultimately used to identify the effect of transcription factors as well as enzyme level manipulations on metabolic fluxes leading to explore key metabolic derivers that underpin various biofuels production

Functional Role of Metal Center and Doping on Tuning the Electrocatalytic Oxygen Evolution Activity of BioMIL-1: Experimental and Theoretical Approaches

To achieve high-efficiency energy conversion and storage technologies, the design and fabrication of high-performance electrocatalysts and an understanding of their catalytic mechanism are critical. In this study, the effect of different metal centers (Co, Ni, Fe, and Cu) on the morphology, structure, and electrocatalytic activity of metal–organic frameworks with nicotinic acid as a linker (Bio-MOFs) on the oxygen evolution reaction (OER) in 0.1 KOH was investigated. In addition, the influence of the second metal on OER was studied. It could be shown that due to the highly exposed bimetal centers and the well-designed architecture of the cobalt–iron (Co–Fe)-based bimetallic–organic framework [BioMIL-(Co–Fe)], the OER showed a low overpotential of 275 mV (10 mA cm–2) and 350 mV (100 mA cm–2), respectively, with a high turnover frequency value of 0.23 s–1 at an overpotential of 320 mV. The presented electrocatalyst creates an emerging class of materials for electrocatalytic applications