2 research outputs found
Dermacozines H–J Isolated from a Deep-Sea Strain of <i>Dermacoccus abyssi</i> from Mariana Trench Sediments
<i>Dermacoccus abyssi</i> sp. nov. strains MT1.1 and
MT1.2 are actinomycetes isolated from a Mariana Trench sediment at
a depth of 10 898 m. The fermentation process using complex
media led to the production of three new pigmented heteroaromatic
(oxidized and reduced) phenazine compounds, dermacozines H–J
(<b>1</b>–<b>3</b>). Extensive use was made of
1D and 2D NMR experiments and high-resolution MS to determine the
structures of the compounds. The new dermacozines showed radical scavenging
activity, and the highest activity was observed for dermacozine H
(<b>1</b>), with an IC<sub>50</sub> value of 18.8 μM
Chaxapeptin, a Lasso Peptide from Extremotolerant <i>Streptomyces leeuwenhoekii</i> Strain C58 from the Hyperarid Atacama Desert
Lasso
peptides are ribosomally synthesized and post-translationally
modified peptides (RiPPs) that possess a unique “lariat knot”
structural motif. Genome mining-targeted discovery of new natural
products from microbes obtained from extreme environments has led
to the identification of a gene cluster directing the biosynthesis
of a new lasso peptide, designated as chaxapeptin <b>1</b>,
in the genome of <i>Streptomyces leeuwenhoekii</i> strain
C58 isolated from the Atacama Desert. Subsequently, <b>1</b> was isolated and characterized using high-resolution electrospray
ionization mass spectrometry and nuclear magnetic resonance methods.
The lasso nature of <b>1</b> was confirmed by calculating its
nuclear Overhauser effect restraint-based solution structure. Chaxapeptin <b>1</b> displayed a significant inhibitory activity in a cell invasion
assay with human lung cancer cell line A549