The SPLASH Survey: Kinematics of Andromeda's Inner Spheroid

The combination of large size, high stellar density, high metallicity, and Sersic surface brightness profile of the spheroidal component of the Andromeda galaxy (M31) within R_proj ~ 20 kpc suggest that it is unlike any subcomponent of the Milky Way. In this work we capitalize on our proximity to and external view of M31 to probe the kinematical properties of this "inner spheroid." We employ a Markov chain Monte Carlo (MCMC) analysis of resolved stellar kinematics from Keck/DEIMOS spectra of 5651 red giant branch stars to disentangle M31's inner spheroid from its stellar disk. We measure the mean velocity and dispersion of the spheroid in each of five spatial bins after accounting for a locally cold stellar disk as well as the Giant Southern Stream and associated tidal debris. For the first time, we detect significant spheroid rotation (v_rot ~ 50 km/s) beyond R_proj ~ 5 kpc. The velocity dispersion decreases from about 140 km/s at R_proj = 7 kpc to 120 km/s at R_proj = 14 kpc, consistent to 2 sigma with existing measurements and models. We calculate the probability that a given star is a member of the spheroid and find that the spheroid has a significant presence throughout the spatial extent of our sample. Lastly, we show that the flattening of the spheroid is due to velocity anisotropy in addition to rotation. Though this suggests that the inner spheroid of M31 more closely resembles an elliptical galaxy than a typical spiral galaxy bulge, it should be cautioned that our measurements are much farther out (2 - 14 r_eff) than for the comparison samples.Comment: Accepted for publication in Ap

A non-apoptotic role for caspase-9 in muscle differentiation

Caspases, a family of cysteine proteases most often investigated for their roles in apoptosis, have also been demonstrated to have functions that are vital for the efficient execution of cell differentiation. One such role that has been described is the requirement of caspase-3 for the differentiation of skeletal myoblasts into myotubes but, as yet, the mechanism leading to caspase-3 activation in this case remains elusive. Here, we demonstrate that caspase-9, an initiator caspase in the mitochondrial death pathway, is responsible for the activation of caspase-3 in differentiating C2C12 cells. Reduction of caspase-9 levels, using an shRNA construct, prevented caspase-3 activation and inhibited myoblast fusion. Myosin-heavy-chain expression, which accompanies myoblastic differentiation, was not caspase-dependent. Overexpression of Bcl-xL, a protein that inhibits caspase-9 activation, had the same effect on muscle differentiation as knockdown of caspase-9. These data suggest that the mitochondrial pathway is required for differentiation; however, the release of cytochrome c or Smac (Diablo) could not be detected, raising the possibility of a novel mechanism of caspase-9 activation during muscle differentiation.</jats:p

Accelerated Particle Swarm Optimization and Support Vector Machine for Business Optimization and Applications

Business optimization is becoming increasingly important because all business activities aim to maximize the profit and performance of products and services, under limited resources and appropriate constraints. Recent developments in support vector machine and metaheuristics show many advantages of these techniques. In particular, particle swarm optimization is now widely used in solving tough optimization problems. In this paper, we use a combination of a recently developed Accelerated PSO and a nonlinear support vector machine to form a framework for solving business optimization problems. We first apply the proposed APSO-SVM to production optimization, and then use it for income prediction and project scheduling. We also carry out some parametric studies and discuss the advantages of the proposed metaheuristic SVM.Comment: 12 page

The young stellar population at the center of NGC 205

Context. NGC 205 is a peculiar dwarf elliptical galaxy hosting in its center a population of young blue stars. Their origin is still matter of debate, the central fresh star formation activity possibly being related to dynamical interactions between NGC 205 and M31. Aims. The star formation history in the central 30\arcsec ($\sim$120 pc) around the NGC 205 central nucleus is investigated in order to obtain clues to the origin of the young stellar population. Methods. Deep HST/ACS CCD photometry is compared with theoretical isochrones and luminosity functions to characterize the stellar content of the region under study and compute the recent SF rate. Results. Our photometry reveals a previously undetected blue plume of young stars clearly distinguishable down to I$\simeq$26. Our analysis suggests that 1.9$\times10^5$ M$_\odot$ were produced between approximately 62 Myr and 335 Myr ago in the NGC 205 inner regions, with a latest minor episode occurring $\sim$25 Myr ago. This implies a star formation rate of $\sim7\times10^{-4}$ M$_\odot$/yr over this period. Conclusions. The excellent fit of the observed luminosity function of young main sequence stars obtained with a model having a constant star formation rate argues against a tidally triggered star formation activity over the last $\sim$300 Myr. Rather, a constant SF may be consistent with NGC 205 being on its first interaction with M 31.Comment: 5 pages, 2 figures, accepted for publication in A&A letter

Polymerase chain reaction detection of avipox and avian papillomavirus in naturally infected wild birds: comparisons of blood, swab and tissue samples

Avian poxvirus (avipox) is widely reported from avian species, causing cutaneous or mucosal lesions. Mortality rates of up to 100% are recorded in some hosts. Three major avipox clades are recognized. Several diagnostic techniques have been reported, with molecular techniques used only recently. Avipox has been reported from 278 different avian species, but only 111 of these involved sequence and/or strain identification. Collecting samples from wild birds is challenging as only few wild bird individuals or species may be symptomatic. Also, sampling regimes are tightly regulated and the most efficient sampling method, whole bird collection, is ethically challenging. In this study, three alternative sampling techniques (blood, cutaneous swabs and tissue biopsies) from symptomatic wild birds were examined. Polymerase chain reaction was used to detect avipoxvirus and avian papillomavirus (which also induces cutaneous lesions in birds). Four out of 14 tissue samples were positive but all 29 blood samples and 22 swab samples were negative for papillomavirus. All 29 blood samples were negative but 6/22 swabs and 9/14 tissue samples were avipox-positive. The difference between the numbers of positives generated from tissue samples and from swabs was not significant. The difference in the avipox-positive specimens in paired swab (4/6) and tissue samples (6/6) was also not significant. These results therefore do not show the superiority of swab or tissue samples over each other. However, both swab (6/22) and tissue (8/9) samples yielded significantly more avipox-positive cases than blood samples, which are therefore not recommended for sampling these viruses.The authors thank bird ringers from Alula and Monticola, especially Alfredo Ortega and Chechu Aguirre, for help with the capture and ringing of birds, which made this project possible. Thanks to Alvaro Ramírez for samples. This research was funded by the Ministerio de Ciencia e Innovación, Spain (grant number: CGL2010-15734/BOS). R.A.J.W. was supported by the Programa Internacional de Captación de Talento (PICATA) de Moncloa Campus de Excelencia Internacional while writing the manuscript

Measurement of Muon Antineutrino Quasi-Elastic Scattering on a Hydrocarbon Target at E_{\nu} ~ 3.5 GeV

We have isolated muon anti-neutrino charged-current quasi-elastic interactions occurring in the segmented scintillator tracking region of the MINERvA detector running in the NuMI neutrino beam at Fermilab. We measure the flux-averaged differential cross-section, d{\sigma}/dQ^2, and compare to several theoretical models of quasi-elastic scattering. Good agreement is obtained with a model where the nucleon axial mass, M_A, is set to 0.99 GeV/c^2 but the nucleon vector form factors are modified to account for the observed enhancement, relative to the free nucleon case, of the cross-section for the exchange of transversely polarized photons in electron-nucleus scattering. Our data at higher Q^2 favor this interpretation over an alternative in which the axial mass is increased.Comment: 8 pages, 5 figures. Added correlation between neutrino and anti-neutrino results in ancillary text files (CSV

Human Papillomavirus E6 Triggers Upregulation of the Antiviral and Cancer Genomic DNA Deaminase APOBEC3B

ABSTRACT Several recent studies have converged upon the innate immune DNA cytosine deaminase APOBEC3B (A3B) as a significant source of genomic uracil lesions and mutagenesis in multiple human cancers, including those of the breast, head/neck, cervix, bladder, lung, ovary, and other tissues. A3B is upregulated in these tumor types relative to normal tissues, but the mechanism is unclear. Because A3B also has antiviral activity in multiple systems and is a member of the broader innate immune response, we tested the hypothesis that human papillomavirus (HPV) infection causes A3B upregulation. We found that A3B mRNA expression and enzymatic activity were upregulated following transfection of a high-risk HPV genome and that this effect was abrogated by inactivation of E6. Transduction experiments showed that the E6 oncoprotein alone was sufficient to cause A3B upregulation, and a panel of high-risk E6 proteins triggered higher A3B levels than did a panel of low-risk or noncancer E6 proteins. Knockdown experiments in HPV-positive cell lines showed that endogenous E6 is required for A3B upregulation. Analyses of publicly available head/neck cancer data further support this relationship, as A3B levels are higher in HPV-positive cancers than in HPV-negative cancers. Taken together with the established role for high-risk E6 in functional inactivation of TP53 and published positive correlations in breast cancer between A3B upregulation and genetic inactivation of TP53, our studies suggest a model in which high-risk HPV E6, possibly through functional inactivation of TP53, causes derepression of A3B gene transcription. This would lead to a mutator phenotype that explains the observed cytosine mutation biases in HPV-positive head/neck and cervical cancers

Kinematic parameters and membership probabilities of open clusters in the Bordeaux PM2000 catalogue

We derive lists of proper-motions and kinematic membership probabilities for 49 open clusters and possible open clusters in the zone of the Bordeaux PM2000 proper motion catalogue ($+11^{\circ}\le\delta\le+18^{\circ}$). We test different parametrisations of the proper motion and position distribution functions and select the most successful one. In the light of those results, we analyse some objects individually. The segregation between cluster and field member stars, and the assignment of membership probabilities, is accomplished by applying a new and fully automated method based on both parametrisations of the proper motion and position distribution functions, and genetic algorithm optimization heuristics associated with a derivative-based hill climbing algorithm for the likelihood optimization. We present a catalogue comprising kinematic parameters and associated membership probability lists for 49 open clusters and possible open clusters in the Bordeaux PM2000 catalogue region. We note that this is the first determination of proper motions for five open clusters. We confirm the non-existence of two kinematic populations in the region of 15 previously suspected non-existent objects.Comment: 14 pages, 6 figures, 4 tables. Accepted for publication in Astronomy & Astrophysic

The Recent Stellar Archeology of M31 - The Nearest Red Disk Galaxy

We examine the star-forming history (SFH) of the M31 disk during the past few hundred Myr. The luminosity functions (LFs) of main sequence stars at distances R_GC > 21 kpc (i.e. > 4 disk scale lengths) are matched by models that assume a constant star formation rate (SFR). However, at smaller R_GC the LFs suggest that during the past ~10 Myr the SFR was 2 - 3 times higher than during the preceding ~100 Myr. The rings of cool gas that harbor a significant fraction of the current star-forming activity are traced by stars with ages ~100 Myr, indicating that (1) these structures have ages of at least 100 Myr, and (2) stars in these structures do not follow the same relation between age and random velocity as their counterparts throughout the disks of other spiral galaxies, probably due to the inherently narrow orbital angular momentum distribution of the giant molecular clouds in these structures. The distribution of evolved red stars is not azimuthally symmetric, in the sense that their projected density along the north east segment of the major axis is roughly twice that on the opposite side of the galaxy. The north east arm of the major axis thus appears to be a fossil star-forming area that dates to intermediate epochs. Such a structure may be the consequence of interactions with a companion galaxy.Comment: To appear in The Astrophysical Journa