The expression of FtsZ and its localization in Spirulina platensis

FtsZ是最早被鉴定出与真核微管蛋白类似的原核骨架蛋白，广泛存在于原核生物中，在植物中也有发现。它最先从大肠杆菌温度敏感型突变体中分离得到，当它突变后杆状大肠杆菌会停止分裂变成长丝状菌体。FtsZ在细胞中部分裂位点形成一个Z环，接着细胞缢缩内陷分裂成两个子细胞。 螺旋藻在不良环境下会从正常螺旋形转变为不同螺旋度的螺旋形甚至直线形。本实验以钝顶螺旋藻（Spirulinaplatensis）FACHB869FtsZ为出发点，研究其功能及与螺旋藻形态建成的关系。我们克隆了钝顶螺旋藻FACHB869的ftsZ基因全序列并构建了表达载体pET28a–ftsZ和荧光融合表达载体pGFPxm-ftsZ。在...FtsZ is a prokaryotic homologue of eukaryotic tubulins. It appears to be universal in eubacteria and archaebacteria and has also been identified in plants. ftsZ has been identified as temperature-sensitive mutants(fts) in Escherichia coli. FtsZ mutants can grow for a time at the non-permissive temperature, but septation is blocked, leading to long filamentous cells. FtsZ forms Z-rings at the centr...学位：理学硕士院系专业：生命科学学院生物学系_水生生物学学号：2162007115206

A Study on the Cooperation Mechanism of the Cross-Strait high-tech industry in the ECFA era

两岸高科技产业的合作机制仅仅是两岸产业合作的一个方面，但不论从动力来源还是运行构成上来说它都是双方合作中最重要以及最核心的一个方面。ECFA时代两岸高科技产业的合作机制出现了一些新特点与新趋势，但仍需从既有理论及文献综述上找到本选题的逻辑起点与理论基础，并按照相关理论所提供的逻辑链条，对两岸高科技产业合作机制进行深入研究。 为了发掘ECFA对两岸高科技产业合作机制的重要意义，本文通过对新世纪以来到ECFA签署前这段时间，两岸高科技产业各自发展的总体态势以及新特点的观察和对比来全面揭示两岸高科技产业的差异性及互补性。通过对ECFA签署前两岸高科技产业业已存在的合作机制的内容与运行进行总结，笔者...Although the Corss-strait high-tech industry cooperation mechanism is just one aspect of Cross-strait industrial cooperation, it is the most significant aspects when we refer to the cooperation of Cross-strait cooperation regardless from its driving force or constitution of operation side. In the ECFA era, there are some new features and tendency coming from the mechanism of Cross-strait high-tech...学位：经济学博士院系专业：台湾研究院_区域经济学学号：2562010015397

基于价值链理论的我国光伏产业动态演进分析

我国光伏产业由光伏电池制造领域切入,到上游晶硅材料领域,再到下游应用领域,形成了一个动态演进的发展路线。但在产业演进的过程中,随着市场份额的扩大,产业的价值创造却在降低,这需要我国厂商在价值链驱动模式的选择上从采购者驱动转向生产者驱动,此外还需要整合产业制造商通过利益交换等方式与渠道商进行价值链治理者的争夺,结合两方面的努力才能改变这一现象并使我国光伏产业得到持续健康的发展。教育部人文社科重点研究基地重大项目(11JJD810006)“后ECFA时代两岸经济合作发展趋势与对策研究”阶段性成

Expression and Localization of FtsZ from Spirulina platensis in Escherichia coli

fTSz是与真核微管蛋白类似的原核骨架蛋白,能在细胞分裂位点聚合组装成环状结构而调控细胞分裂过程。为了研究钝顶螺旋藻(SPIrulInA PlATEnSIS)fTSz蛋白的功能,构建了钝顶螺旋藻fTSz与绿色荧光蛋白gfP融合表达的质粒,并在大肠杆菌中进行了表达和定位研究,结果发现,表达融合蛋白gfP-fTSz的大肠杆菌细胞由短杆状变为长丝状,且菌丝体长度与融合蛋白的表达量呈正比。在荧光显微镜下观察到融合蛋白gfP-fTSz在长丝状体细菌中呈有规律的点状分布,这说明fTSz蛋白功能高度保守,钝顶螺旋藻fTSz蛋白能识别大肠杆菌分裂位点并装配成环状结构调控大肠杆菌细胞分裂,fTSz蛋白的过量表达能抑制大肠杆菌正常的细胞分裂而导致长丝状体细胞的形成。Prokaryotic cytoskeletal protein FtsZ,a microtubule homolog,assembles into a compact circular structure at the mid-cell and plays an important role in cytokinesis.To explore the function of FtsZ in Spirulina platensis morphogenesis,we cloned the ftsZ gene from S.platensis and constructed its fusion tag of GFP expression plasmid pGFP-FtsZ.The recombined expression vector was transformed to Escherichia coli BL21.Western blot analysis showed that the GFP-FtsZ fusion gene was successfully expressed in the transformant.The transformed bacteria that expressed the GFP-FtsZ protein changed from normal short-rod shapes and formed long filaments.The length of the filamentation cells was proportional to the expression amount of FtsZ in cells.Regular-dot distribution of the GFP-FtsZ fusion protein in transformed bacteria was observed by fluorescent light microscopy.The data demonstrated that FtsZ was a highly conserved functional protein.The FtsZ of S.platensis assembled the complete cytokinesis apparatus and formed a Z-ring structure at the future division site to regulate cell division in E.coli.The overexpression of FtsZ blocked the normal cell cycle and led to cell filamentation.国家自然科学基金资助项目(30571446); 福建省自然科学基金资助项目(2009J01193

Expression profile of voltage-gated potassium channel Kv1.3 in aorta of atherosclerosis rats

目的探讨动脉粥样硬化(AS)大鼠主动脉病变局部离子通道kV1.3的表达水平及作用。方法雄性WISTAr大鼠16只,随机分为两组:正常组(8只,予普通饮食+0.9%氯化钠溶液)和AS组(8只,予高脂饮食+维生素d3负荷)。采用组织病理学检查,观察主动脉粥样硬化病变。采用实时定量反转录-聚合酶链反应(rT-PCr)和WESTErn印迹法检测主动脉病变局部kV1.3和白细胞介素(Il)-2、干扰素(Ifn)-γ的表达水平。结果组织病理学检查证实纤维增生性AS斑块。AS组的kV1.3MrnA为(31.48±8.64)x10-3,显著高于正常组的(3.28±0.79)x10-3(P=0.012)。AS组的kV1.3、Il-2、Ifn-γ蛋白表达量分别为0.691±0.067、0.611±0.055、0.759±0.050,均显著高于正常组的0.490±0.052、0.299±0.058、0.273±0.052(P值均<0.01)。结论 kV1.3在主动脉粥样硬化病变局部的表达增高。kV1.3可能在AS的发生和发展过程中发挥着重要的作用。Objective To investigate the expression of voltage-gated potassium channel Kv1.3 in the aorta of a rat model of atherosclerosis and its role in the progress of atherosclerotic plaque formation.Methods A total of 16 male Wistar rats were randomly divided into normal control(normal diet and saline)and atherosclerosis group(high lipid diet+Vitamin D3 overload),with 8 rats in each group.In 16 weeks later,all rats were killed after weighing,and their blood samples and aorta were collected.Pathological changes of the rat aortic artery were observed with HE staining.Real time RT-PCR and Western blotting analysis were used to determine the mRNA and protein expression of Kv1.3 and the protein expressions of interleukin(IL)-2 and interfereron(IFN)-γ.Results Pathological changes showed that fiber proliferative atherosclerotic plaques were found in the aorta of atherosclerosis group,with inflammatory cells infiltrating in the local lesion.Real time RT-PCR analysis showed that the expression of Kv1.3 mRNA in the aorta was increased significantly in the atherosclerosis rats than that in the controls([31.48±8.64]×10-3 vs.[3.28±0.79]×10-3,P<0.05).Western blotting analysis showed that the protein expression of Kv1.3,IL-2 and IFN-γ in the aorta were also increased significantly in the atherosclerotic rats than that in the controls(Kv1.3[0.691±0.067] vs.[0.490±0.052],IL-2 [0.611±0.055] vs.[0.299±0.058],IFN-γ [0.759±0.050] vs.[0.273±0.052],P<0.01 n=8).Conclusion The expression of Kv1.3 potassium channels is increased in the plaques of atherosclerotic rats.Kv1.3 may play an important role in the development and progression of atherosclerosis.国家自然科学基金资助项目(30871045

短小芽孢杆菌、分离方法、糖脂类表面活性剂制备方法

本发明提供了一种短小芽孢杆菌，所述芽孢杆菌的保藏号为CCTCC?M2015438。本发明的提供的短小芽孢杆菌，具有降解石油组分和产生表面活性剂的双功能，拓展了生物医疗微生物的范围。本发明提供的糖脂类表面活性剂的制备方法，采用海水加营养物配置成廉价寡养培养基，进行海洋细菌短小芽孢杆菌L-2菌株的产生物表面活性剂糖脂发酵，有效降低了发酵成本

海洋芽孢杆菌、海洋右旋糖苷酶及其应用

本发明的提供了一种海洋芽孢杆菌，将其应用于药物开发、日用化妆品、中，拓展了生物医疗微生物的范围。通过在海洋芽孢杆菌(Bacillus?sp.)L-1中提取分离出海洋右旋糖苷酶的基因及其表达翻译的右旋糖苷酶，与其它的右旋糖苷酶相比，本发明所述的海洋右旋糖苷酶来源于海洋低温高盐环境，该酶在25~30℃海水中具有很高的右旋糖苷降解效率，常温下就可以发挥作用，而且40℃以上极不稳定，中温就可以使酶失活，从而确保了其安全性

The Cooperation Situation and Mechanism Innovation of Cross-strait High-tech Industry

2008年以来,两岸高科技产业合作有了突破性的进展,衍生了合作机制创新的诉求。笔者通过对现今两岸高科技产业合作新态势的观察,对两岸高科技产业合作机制的内容、构成与运行方式进行总结,并提出两岸高科技产业可能达成的合作新机制。Since 2008,the improvement of cross-strait political environment makes the cross-strait industrial cooperation in high-tech industry with breakthrough development,the scope of cross-strait cooperation in high-tech industry continues to expand with further deepening the level of its cooperation.However,the past market mechanism with the absence of government functions can not meet the requirements of cross-strait cooperation in high-tech industry,and gives rise to the demands of innovation and cooperation mechanisms.The author,through the observation of situation of the current cross-strait cooperation,summarizes the content structure and way of running in cross-strait high-tech industry,and on the current defect basis,proposes a new mechanism of cooperation in cross-strait high-tech industry.国家科社基金重点项目“构建台湾海峡两岸关系和平发展框架研究”(11AZZ005)阶段性成果之

海洋芽孢杆菌、分离方法、海洋右旋糖苷酶制备方法及应用

本发明提供了一种海洋芽孢杆菌，所述芽孢杆菌的保藏号为CCTCC?M2015437。本发明应用于上述海洋芽孢杆菌制备得到海洋右旋糖苷酶，对变异链球菌具有抑制其生物膜形成的功能，除了可以应用于龋齿防治及在医疗，日化等方面以外，对海洋生物医药等方面也具有巨大的应用潜力