141 research outputs found

    Effects of the Xitongxiao prescription on apoptosis and proliferation of chondrocytes in knee

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    目的:探讨膝痛消方对促进软骨细胞增殖和抑制IL-1β诱导的软骨细胞凋亡的作用。方法:体外培养膝关节软骨细胞,用阿利新蓝染色方法检测软骨细胞蛋白多糖,用Ⅱ型胶原免疫荧光染色检测软骨细胞Ⅱ型胶原的表达,以鉴定软骨细胞;用MTS检测软骨细胞的增殖;用IL-1β诱导软骨细胞凋亡,AnnexinV/PI双染检测细胞凋亡。结果:膝关节剥离得到的细胞形态为多角形或梭形,阿利新蓝与Ⅱ型胶原免疫荧光染色均呈阳性,证明分离培养的细胞为软骨细胞。MTS实验结果发现膝痛消方提取物处理软骨细胞后检测到的OD值随浓度增加而增加且显著高于对照组。此外,IL-1β处理诱导软骨细胞凋亡,而膝痛消方提取物能够有效地抑制IL-1β引起的细胞凋亡。结论:膝痛消方能促进软骨细胞的增殖,抑制IL-1β诱导的软骨细胞的凋亡。以上发现为膝痛消方临床上治疗膝关节骨性关节炎提供药理作用依据。Objective:To investigate the effects of the Xitongxiao prescription on the cell proliferation and the inhibition of IL-1β-induced apoptosis of chondrocytes.Methods:To identify chondrocytes,the articular chondrocytes were cultured in vitro;staining with alcian blue was used to detect the protein polysaccharide,and staining with collagen immunofluorescence was used to examine the expression of type II collagen in chondrocytes.The proliferation of chondrocytes was determined by MTS.Apoptosis of chondrocytes was induced by IL-1β,and the effect of apoptosis was tested by Annexin V/PI double staining.Results:The morphology of the cells obtained from the knee joint was polygonal or fusiform.The tested results of Alcian blue and collagen immunofluorescence staining were positive.These together certified that the isolated cells were knee chondrocytes.MTS assay results showed that the detected OD values were dosedependently increased and they were significantly higher than those of the control group.In addition,the apoptosis of chondrocytes was induced by IL-1βtreatment.While the extract of the Xitongxiao prescription could effectively inhibit the IL-1β-induced apoptosis of chondrocytes.Conclusion:The Xitongxiao prescription could promote the proliferation of chondrocytes and inhibit the apoptosis of chondrocytes induced by IL-1β.The above findings may provide one of pharmacological mechanisms of the Xitongxiao for clinical treatment of knee osteoarthritis.厦门市科技计划项目(3502z20144031);福建省自然科学基金计划资助项目(2015J01065)

    Degradation of HPAM-containing wastewater with integrated process of UASB and immobilized microorganism reactor

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    随着聚合物驱油技术在油田的广泛推广,部分水解聚丙烯酰胺(HPAM)在为油田提高采收率的同时,对当地环境也产生了相当大的危害。本文在对含聚污水水质分析和可生化性分析的基础上对污水进行可生化性调整,运用“气浮-uASb-水解酸化-微生物固定化反应器“组合工艺对含聚污水进行了生化处理模拟实验。模拟实验分为静态模拟和动态模拟两部分。静态模拟实验中,降解6天后,聚丙烯酰胺降解率达到89.7%。动态模拟试验中,组合工艺处理2天以后,HPAM降解率为88.65%,原油总去除率为99.40%,出水COd总去除率为93.40%。利用扫描电镜(SEM)和红外光谱分析聚丙烯酰胺降解产物,显示HPAM由大分子物质断裂成小分子物质,HPAM的酰胺基转化为羧基。Polymer flooding technique has become an important method of enhanced oil recovery.But the application of partially hydrolyzed polyacrylamide(HPAM) in oilfield meets many environmental problems.Based on water analysis and biodegradability analysis of HPAM-containing wastewater,improved biodegradability of wastewater was achieved.Laboratory simulation treatment of wastewater was performed by the "flotation-up-flow anaerobic sludge bed(UASB)-hydrolysis acidificationimmobilized microorganism reactor" integrated process.There were two parts in simulation experiment,one was static,and the other was dynamic.After 2 d static biochemical treatment,the degradation ratio of HPAM in wastewater was up to 89.7%.And in dynamic simulation experiment,after 2 d treatment,the degradation ratio of HPAM was up to 88.65%,that of oil was 99.40%,that of COD was 93.40%.HPAM degradation products were analyzed by scanning electron microscopy(SEM) and infrared spectroscopy.The results showed that HPAM was transformed from bigger molecules into smaller molecules and acylamino group in HPAM was transformed into carboxyl group.国家863计划项目(2008AA06Z304

    复现高超声速飞行条件下10°尖锥标模气动力特性试验研究

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    综述了应用中科院力学所的JF12长试验时间激波风洞中,在复现40km高度,飞行马赫数7的试验条件下,开展的10°尖锥标模天平测力试验研究结果

    产胞外布雷菲德菌素A青霉发酵条件的研究

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    通过正交设计试验,优化了青霉(Penicillium)属真菌SHZK-15产胞外布雷菲德菌素(B refeld in-A,简称BFA)的液体发酵条件。最佳的BFA发酵条件为:培养基含马铃薯200g(煮汁),葡萄糖20g,(NH4)2SO44.0g,KH2PO41.0g,MgSO4.7H2O 2.0g,CaCO35.0g,pH自然,瓶装量为100mL/250 mL,培养温度为28℃,转速为120 r/m in,培养时间为7d,BFA的最高产量可达151.6mg/L

    活心丸(浓缩丸)治疗冠心病稳定性心绞痛的多中心、随机、双盲、安慰剂对照临床研究

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    目的观察活心丸(浓缩丸)治疗冠心病稳定性心绞痛的临床疗效和安全性。方法本研究采用多中心、随机、双盲、安慰剂对照研究方法,将480例气虚血瘀型冠心病稳定性心绞痛患者以3:1比例随机分为试验组(360例)和对照组(120例)。在西医常规治疗基础上,两组患者分别服用活心丸(浓缩丸)和安慰剂,1次2粒,每日3次;疗程8周。观察两组治疗前后主要指标(心绞痛症状积分)和次要指标(包括硝酸甘油减停率、中医证候积分及生活质量评价)及安全性。结果共454例患者完成试验(试验组336例,对照组118例)。与本组治疗前比较,试验组和对照组心绞痛症状积分和中医证候积分均降低,西雅图心绞痛量表评分改善(P<0.01);与对照组治疗后比较,试验组心绞痛症状积分、中医证候积分、西雅图心绞痛量表评分改善均优于对照组(P<0.01)。试验组心绞痛症状疗效总有效率为80.95%,硝酸甘油停减率为80.70%,中医证候疗效有效率80.65%,均高于对照组[36.44%、45.07%、38.99%(χ~2=58.21、40.94、66.55,P<0.01)]。试验组中既往有心梗史患者心绞痛症状总有效率为83.22%,中医证候有效率83.22%,西雅图心绞痛量表评分为(361.74±62.10)分,均高于无心梗史患者[60.92%、66.89%、(327.95±65.07)分(χ~2=13.89、13.26,P<0.01)]。治疗过程中未发生明显不良反应。结论活心丸(浓缩丸)治疗气虚血瘀型冠心病稳定性心绞痛疗效显著,尤其适用于既往有心梗病史的冠心病稳定性心绞痛患者,无明显不良反应。国家中医药管理局资助课题(No.国中医药科2016ZX04

    Photochemical route for synthesizing atomically dispersed palladium catalysts

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    该工作由校内外多个课题组共同努力,历时三年多完成。我校郑南峰、傅钢等课题组紧密协作负责催化剂的合成、表征、催化测试及机理研究;中科院物理研究所谷林研究员主要负责催化剂的球差校正透射电子显微研究;加拿大达尔豪斯大学的张鹏课题组参与催化剂的同步辐射X-射线吸收谱研究。该研究工作的第一、二作者刘朋昕、赵云均为我校博士生。【Abstract】Atomically dispersed noble metal catalysts often exhibit high catalytic performances, but the metal loading density must be kept low (usually below 0.5%) to avoid the formation of metal nanoparticles through sintering. We report a photochemical strategy to fabricate a stable atomically dispersed palladium–titanium oxide catalyst (Pd 1 /TiO2 ) on ethylene glycolate (EG)–stabilized ultrathin TiO2 nanosheets containing Pd up to 1.5%.The Pd 1 /TiO2 catalyst exhibited high catalytic activity in hydrogenation of C=C bonds, exceeding that of surface Pd atoms on commercial Pd catalysts by a factor of 9.No decay in the activity was observed for 20 cycles. More important, the Pd 1 /TiO2 -EG system could activate H2 in a heterolytic pathway, leading to a catalytic enhancement in hydrogenation of aldehydes by a factor of more than 55.Supported by Ministry of Science and Technology of China grant 2015CB932303; National Natural Science Foundation of China grants 21420102001, 21131005, 21390390, 21133004, 21373167, 21573178, and 21333008; a NSERC CGS Alexander Graham Bell scholarship (D.M.C.); and a NSERC Discovery grant (P.Z.)

    融合结构信息的Hausdorff距离在图像匹配中的应用

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    现有的基于Hausdorff距离的边缘图像匹配主要利用边缘的位置信息,忽视了边缘的其他有用信息。为了提高基于边缘的图像匹配的鲁棒性,采用Haussdorff距离与结构信息相结合的方法,并给出了相似性度量函数。利用可见光图像进行仿真,验证了此方法的有效性和鲁棒性

    基于遥感案例推理的海岸带养殖信息提取

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    目前基于目视解释或光谱分类的养殖信息提取效率低,难以克服由于地物混杂带来的椒盐噪声现象且难以融合地学知识。针对养殖信息提取中存在的问题,首先在分析现有养殖信息提取方法和案例推理CBR(Case-Based Reasoning)用于遥感图像处理的基础上,提出基于遥感案例推理的海岸带养殖信息提取的研究思路;其次,结合养殖区域的空间特征和属性特征,构建案例的表达模型以及CBR相似性推理模型;最后,对不属于案例构建区的粤西沙田镇进行养殖信息提取的CBR实验,精度达到84.56%。对比CBR方法和传统监督分类方法可知,CBR方法是实现海岸带养殖信息快速准确提取的一种有效手段

    使用多层深度采样的屏幕空间环境光遮挡

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    为解决传统的基于水平线的屏幕空间环境光遮挡算法不能准确计算被遮挡物体的环境光遮挡值的问题,提出了一种使用多层深度采样的屏幕空间环境光遮挡算法。该算法使用桶型深度剥离算法获取场景中各层次的深度信息,并使用这些信息为场景中所有物体计算环境光遮挡值,有效解决了算法不能为被遮挡物体计算环境光遮挡值的问题。该算法的计算过程完全在图形处理器中完成。实验结果表明,该算法相对于原算法具有更好的真实感
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