33 research outputs found

    Comparison of Invasive Blood Pressure Measurements from the Caudal Ventral Artery and the Femoral Artery in Male Adult SD and Wistar Rats

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    <div><p>Background and Purpose</p><p>Studies have suggested that the caudal ventral artery is a potential site for continuous arterial blood pressure monitoring in rats. However, the agreement of mean arterial pressure values between the femoral artery and the caudal ventral artery has not been investigated. This study was performed to identify whether the caudal ventral artery could be safely used for continuous blood pressure monitoring as an alternative site to the femoral artery.</p> <p>Methods</p><p>Rats were randomized into four groups: Sprague Dawley rats under normothermia; Wistar rats under normothermia; Sprague Dawley rats under hypothermia; Wistar rats under hypothermia. Each rat underwent simultaneous monitoring of blood pressure using femoral artery and caudal ventral artery catheterization during a stable hemodynamic state and three periods of acute severe hemodynamic changes. The effects of rat strain, rectal temperature, experimental time course and hemodynamic factors on pressure gradients, the concordance of mean arterial pressure values between the femoral artery and the caudal ventral artery, and the rates of distal ischemia after surgery were determined.</p> <p>Results</p><p>There was a significant difference in the rate of distal ischemia between femoral and caudal ventral arteries after catheterization (25% <i>vs</i> 5%, <i>P</i><0.05). The overall mean gradient and the mean gradient under a steady hemodynamic state were 4.9±3.7 mm Hg and 5.5±2.5 mm Hg, respectively. The limits of agreement (bias±1.96 SD) were (−2.5 mm Hg, 12.3 mm Hg) and (-0.5 mm Hg, 10.5 mm Hg), respectively. Although the concordance decreased during the first 30 sec of each period of severe hemodynamic changes, the degree of agreement was acceptable regardless of the effects of rat strain and rectal temperature.</p> <p>Conclusions</p><p>Based on the degree of agreement and the safety of catheterization, the caudal ventral artery may be a preferred site for continuous arterial blood pressure monitoring without acute severe hemodynamic changes.</p> </div

    The surgical procedures and simultaneous measurements of BP.

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    <p>The surgical procedures and simultaneous measurements of BP.</p

    Physiological parameters of inclusion criteria.

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    <p>The presented values are the means ± SD. DN, SD rats under normothermia; DH, SD rats under hypothermia; WN, Wistar rats under normothermia; WH, Wistar rats under hypothermia.</p

    Bland-Altman parameters for concordance between MAP values measured at FA and CVA at all the time points.

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    <p>CI, Confidence Interval; Percent Error, Percent Exceeding the 95% CI of the overall difference; <i>r</i>, Correlation coefficient; Upper and Lower, the upper and the lower limit of the 95% CI; T<sub>0</sub> = 0 sec of each treatment; T<sub>1</sub> = 15 sec after starting each treatment; T<sub>2</sub> = 30 sec after starting each treatment; T<sub>3</sub> = 45 sec after starting each treatment; T<sub>4</sub> = 60 sec after starting each treatment; T<sub>5</sub> = 75 sec after starting each treatment; T<sub>6</sub> = 90 sec after starting each treatment; T<sub>7</sub> = 105 sec after starting each treatment; T<sub>8</sub> = 120 sec after starting each treatment; MAP, mean arterial pressure; FA, femoral artery; CVA, caudal ventral artery. Superscript(*), disagreement according to the overall 95% CI; Superscript(<sup>#</sup>), number and percentage of the plots that exceed the overall 95% CI; Superscript(<sup>△</sup>), the frequency of individual gradients greater than those that indicate good for agreement.</p

    Participants’ sensitivity (<i>d’</i>) in each ethnic group for each condition.

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    <p>Participants’ sensitivity (<i>d’</i>) in each ethnic group for each condition.</p

    MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

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    The chemotherapy resistance often leads to chemotherapy failure. This study aims to explore the molecular mechanism by which MUC1 regulates paclitaxel resistance in lung adenocarcinoma (LUAD), providing scientific basis for future target selection. The bioinformatics method was used to analyse the mRNA and protein expression characteristics of MUC1 in LUAD. RT-qPCR and ELISA were used to detect the mRNA and protein expression, flow cytometry was used to detect CD133+ cells, and cell viability was detected by CCK-8 assay. The mRNA-seq was performed to analyse the changes in expression profile, GO and KEGG analysis were used to explore the potential biological functions. MUC1 is highly expressed in LUAD patients and is associated with a higher tumour infiltration. In paclitaxel resistance LUAD cells (A549/TAX cells), the expression of MUC1, EGFR/p-EGFR and IL-6 were higher than that of A549 cells, the proportion of CD133+ cells was significantly increased, and the expression of cancer stem cell (CSCs) transcription factors (NANOG, OCT4 and SOX2) were significantly up-regulated. After knocking down MUC1 in A549/Tax cells, the activity of A549/Tax cells was significantly decreased. Correspondingly, the expression of EGFR, IL-6, OCT4, NANOG, and SOX2 were significantly down-regulated. The mRNA-seq showed that knocking down MUC1 affected the gene expression, DEGs mainly enriched in NF-κB and MAPK signalling pathway. MUC1 was highly expressed in A549/TAX cells, and MUC1-EGFR crosstalk with IL-6 may be due to the activation of NF-κB and MAPK pathways, which promote the enrichment of CSCs and lead to paclitaxel resistance.</p

    In-group trust and out-group distrust.

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    <p><i>d’</i> values for Han and Uyghur participants toward <i>In−/out-group+Trust/Distrust</i>. Error bars denote standard error of mean (S.E.M.). <sup>*</sup> indicates p<.05, <sup>***</sup> indicates p<.001, <sup>#</sup> indicates p<.10.</p

    Neuroanatomical correlates of interval perception in the amygdala.

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    <p>The peak voxel is located in the left amygdala. The scatter plot shows distributions of scores on interval perception vs. mean GMV of all the voxels in the cluster after controlling for sex and total GMV. Each dot represents one participant.</p