370 research outputs found

    A Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (<em>Argopecten irradians</em>) and Its Application in Size-Related QTL Analysis

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    <div><p>Bay scallop (<em>Argopecten irradians</em>) is one of the most economically important aquaculture species in China. In this study, we constructed a consensus microsatellite-based genetic linkage map with a mapping panel containing two hybrid backcross-like families involving two subspecies of bay scallop, <em>A. i. irradians</em> and <em>A. i. concentricus</em>. One hundred sixty-one microsatellite and one phenotypic (shell color) markers were mapped to 16 linkage groups (LGs), which corresponds to the haploid chromosome number of bay scallop. The sex-specific map was 779.2 cM and 781.6 cM long in female and male, respectively, whereas the sex-averaged map spanned 849.3 cM. The average resolution of integrated map was 5.9 cM/locus and the estimated coverage was 81.3%. The proportion of distorted markers occurred more in the hybrid parents, suggesting that the segregation distortion was possibly resulted from heterospecific interaction between genomes of two subspecies of bay scallop. The overall female-to-male recombination rate was 1.13∢1 across all linked markers in common to both parents, and considerable differences in recombination also existed among different parents in both families. Four size-related traits, including shell length (SL), shell height (SH), shell width (SW) and total weight (TW) were measured for quantitative trait loci (QTL) analysis. Three significant and six suggestive QTL were detected on five LGs. Among the three significant QTL, two (<em>qSW-10</em> and <em>qTW-10</em>, controlling SW and TW, respectively) were mapped on the same region near marker <em>AiAD121</em> on LG10 and explained 20.5% and 27.7% of the phenotypic variance, while the third (<em>qSH-7</em>, controlling SH) was located on LG7 and accounted for 15.8% of the phenotypic variance. Six suggestive QTL were detected on four different LGs. The linkage map and size-related QTL obtained in this study may facilitate marker-assisted selection (MAS) in bay scallop.</p> </div

    Mapping significant size-related QTL.

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    <p>A, QTL for shell length on LG7; B, QTL for total weight on LG10. QTL were detected with genome-wide threshold. The QTL names are on the left of each box-bar. The significance of Kruskal-Wallis analyses are shown at the right side of linkage group (**<i>P</i><0.05, *** <i>P</i><0.01, ****<i>P</i><0.005, ***** <i>P</i><0.001).</p

    Experimental mating scheme employed to generate the two hybrid backcross-like mapping families (CC5 and CC10) and segregation of shell color in offspring.

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    <p>Experimental mating scheme employed to generate the two hybrid backcross-like mapping families (CC5 and CC10) and segregation of shell color in offspring.</p

    Information of the analyzed objects.

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    <p><sup>a</sup> NO nonessential genes information provided in DEG.</p><p>Information of the analyzed objects.</p

    Significance levels of 33 objects in a normal distribution based on the hurstSpec method in smoothed mode.

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    <p>Significance levels of 33 objects in a normal distribution based on the hurstSpec method in smoothed mode.</p

    Q–Q plots of the Hurst exponents.

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    <p>A, B, and C: <i>Escherichia coli</i> MG1655 I; D, E, and F: <i>Salmonella enterica subsp</i>. <i>enterica serovar Typhimurium str</i>. 14028S. A and D show Q–Q plots of the Hurst exponents of the full-gene-sets of the two objects from respective organisms. B and E show Q–Q plots of the Hurst exponents of the essential genes of the two objects from respective organisms. C and F show Q–Q plots of the Hurst exponents of the nonessential genes of the two objects from respective organisms.</p

    Consensus microsatellite-based linkage map for bay scallop (<i>Argopecten irradians</i>) constructed using two hybrid backcross-like families.

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    <p>The female linkage group (left) is named as β€œAiF_LG”; the male linkage group (middle) is named as β€œAiM_LG”; the sex-averaged linkage group (right) is named as β€œAi_LG”. The phenotypic maker (shell color) placed on LG 10 was underlined.</p

    Number of markers and genetic length for sex-averaged linkage map of bay scallop.

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    <p>Number of markers and genetic length for sex-averaged linkage map of bay scallop.</p

    Size-related QTL identified in two families (CC5 and CC10) of bay scallop.

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    <p>SL, shell length; SH, shell height; TW, total weight; PVE, percentage of phenotypic variance explained by the QTL; association between four parental genotypes (f1m1, f2m1, f1m2, f2m2) of markers nearest each QTL peak and mean phenotypic values of each trait was tested by Kruskal-Wallis analysis.</p>#<p>Confidence interval at 95% significance;</p>†<p>linkage-group-wide;</p>‑<p>genome wide;</p><p>*P<0.1, **<i>P</i><0.05, *** <i>P</i><0.01, ****<i>P</i><0.005, ***** <i>P</i><0.001.</p
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