2,162 research outputs found

    LOCATION STRATEGIES BASED ON DISCRETE CHOICE MODELS: AN EMPIRICAL APPLICATION TO SUPERMARKET LOCATION

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    In this paper we present a theoretic two-stage model for retailers location and consumers purchase decision. Retailers decision problem is formalized in terms of a zero-sum game, whose payoffs refers to retailers' market share and consumers decision problem is formalized in terms of a discrete choice model, based on random utilities. The theoretical models provide forecasting of equilibrium market shares and the locations to be chosen by retailers, in terms of the geographic distribution of the underlying location space (constituencies of the town), population distribution and characteristics (types) of the consumers.Hotelling, Industrial Organization, Choice Model

    Analizando la variabilidad del registro arqueológico en sociedades cazadoras-recolectoras desde la etnoarqueología

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    Desde 1986, nuestros proyectos de investigaci√≥n (en colaboraci√≥n con una contraparte argentina) en la Isla Grande de Tierra del Fuego han tenido como objetivo destacado el desarrollo de una metodolog√≠a para un adecuado reconocimiento de la organizaci√≥n social a partir de la evidencia arqueol√≥gica. El trabajo aqu√≠ presentado se concentra en uno de los asentamientos excavados durante estos proyectos, T√ļnel VII, ubicado en la parte argentina de la Isla Grande de Tierra del Fuego. El yacimiento, un ¬ęconchero¬Ľ, es altamente representativo de las sociedades canoeras de los canales fueguinos, al menos en su fase final. El m√©todo de excavaci√≥n nos ha permitido reconocer la detallada secuencia de una serie de, al menos, diez episodios discretos de ocupaci√≥n a lo largo de un siglo, que tuvieron lugar en la pr√°ctica totalidad de las estaciones del a√Īo. Los momentos de ocupaci√≥n de T√ļnel VII no llevan vinculadas espec√≠ficas pautas estacionales. Son estrategias de subsistencia especializadas pero ¬ęflexibles¬Ľ que no se ven representadas en la estructura conceptual dual ¬ęcollector¬Ľ o ¬ęforager¬Ľ, es decir, una ¬ęespecializaci√≥n en la no especializaci√≥n¬Ľ. La misma evidencia ha servido para fijar claros y definitorios comportamientos generales respecto al uso del espacio.From 1986, our research projects in Isla Grande of Tierra del Fuego, in collaboration with other Argentinian team, have emphasized the goal of developing a methodology for a proper archaeological recognition of the social organization from archaeological evidence. The work presented here focuses on one of the settlements excavated during these projects, VII Tunnel, located on the Argentine side of the Isla Grande of Tierra del Fuego. The site is highly representative of the canoe societies of the fueguian channels, at least in its final phase. The excavation method allow us to recognize the detailed sequence of a series of at least ten discrete episodes of occupation, over a century, which took place in almost all the seasons. The Tunnel VII moments of occupation do not carry specific seasonal patterns linked. Livelihood strategies are highly specialized but "flexible" that are not represented in the conceptual dual "collector" or "forager", ie "specialization in non-specialization". The same evidence has served to set clear and defining general behavior regarding the use of space

    RAZVOJ HOMOZIGOTNIH LINIJA KUKURUZA POMOńÜU INDUKCIJE HAPLOIDA IN VIVO U HRVATSKOJ GERMPLAZMI

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    The in vivo haploid induction has been widely applied to the maize breeding in recent decades, but it has not been used in the breeding programs in the Republic of Croatia by now. This study\u27s objectives were to examine the haploid induction rates in the Croatian germplasm and to evaluate the properties of the D0 haploids, which are essential for a successful implementation of this method in breeding. The in vivo haploid induction was performed on 11 single-cross hybrids using the Zarodyshevy Marker Krasnodarsky (ZMK) inducer, and colchicine was used as a chromosome doubling agent. Emergence, misclassification rate, colchicine treatment survival, chromosome doubling rate and self-pollination success were examined in the D0 generation. The haploid induction rates ranged from 6.9 to 15.8%, which is consistent with the average induction rates characteristic of the ZMK inducer and the other modern ones. Significant differences were found among the populations of D0 haploids for all tested properties, except for self-pollination success. On average, the misclassification rates were lower, and the seedling survival rates were higher than those reported in other studies, indicating a possibility of a successful application of the doubled haploid method in maize breeding.Indukcija haploida in vivo posljednjih se desetljeńáa sve viŇ°e primjenjuje u oplemenjivanju kukuruza, ali se dosad nije koristila u oplemenjivańćkim programima u Republici Hrvatskoj. Ciljevi ovoga istraŇĺivanja bili su utvrditi relativne frekvencije induciranih haploida kod germplazme koja se upotrebljava u domańáim oplemenjivańćkim programima i ispitati svojstva haploida generacije D0 koja su vaŇĺna za uspjeŇ°nu primjenu ove metode u oplemenjivanju. Indukcija haploida in vivo obavljena je na 11 jednostrukih hibrida pomońáu induktora ZMK, a za udvostruńćenje kromosoma koriŇ°ten je kolhicin. U generaciji D0 ispitana su svojstva nicanja, pogreŇ°ne klasifikacije, preŇĺivljavanja tretmana kolhicinom, udvostruńćenja kromosoma i uspjeŇ°nosti izvońĎenja samooplodnje. Relativna frekvencija haploida kretala se od 6,9 do 15,8%, Ň°to je usporedivo s prosjeńćnim stopama indukcije specifińćnim za induktor ZMK i druge moderne induktore. Statistińćki znańćajne razlike pronańĎene su izmeńĎu populacija haploida D0 za sva ispitana svojstva, osim za uspjeŇ°nost izvońĎenja samooplodnje. U prosjeku, stope pogreŇ°ne klasifikacije bile su niŇĺe, a stope preŇĺivljavanja tretmana kolhicinom viŇ°e u odnosu na one zabiljeŇĺene u drugim istraŇĺivanjima, Ň°to ukazuje na moguńánost uspjeŇ°ne primjene metode udvostruńćenih haploida u oplemenjivanju

    Cytoplasmic Expression of the ALS/FTD-Related Protein TDP-43 Decreases Global Translation Both in vitro and in vivo

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    TDP-43 is a major component of cytoplasmic inclusions observed in neurodegenerative diseases like frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). To further understand the role of TDP-43 in mRNA/protein metabolism and proteostasis, we used a combined approach with cellular and animal models overexpressing a cytoplasmic form of human TDP-43 (TDP-43-őĒNLS), recapitulating ALS/FTD features. We applied in HEK293 cells a method for labeling de novo translation, surface sensing of translation (SUnSET), based on puromycin (PURO) incorporation. While control cells displayed robust puromycilation, TDP-43-őĒNLS transfected cells exhibited reduced ongoing protein synthesis. Next, by using a transgenic mouse overexpressing cytoplasmic TDP-43 in the forebrain (TDP-43-őĒNLS mice) we assessed whether cytoplasmic TDP-43 regulates global translation in vivo. Polysome profiling of brain cortices from transgenic mice showed a shift toward non-polysomal fractions as compared to wild-type littermates, indicating a decrease in global translation. Lastly, cellular level translational assessment by SUNSET was performed in TDP-43-őĒNLS mice brain slices. Control mice slices incubated with PURO exhibited robust cytoplasmic PURO signal in layer 5 neurons from motor cortex, and normal nuclear TDP-43 staining. Neurons in TDP-43-őĒNLS mice slices incubated with PURO exhibited high cytoplasmic expression of TDP-43 and reduced puromycilation respect to control mice. These in vitro and in vivo results indicate that cytoplasmic TDP-43 decreases global translation and potentially cause functional/cytotoxic effects as observed in ALS/FTD. Our study provide in vivo evidence (by two independent and complementary methods) for a role of mislocalized TDP-43 in the regulation of global mRNA translation, with implications for TDP-43 proteinopathies.Fil: Charif, Santiago El√≠as. Consejo Nacional de Investigaciones Cient√≠ficas y T√©cnicas. Oficina de Coordinaci√≥n Administrativa Houssay. Instituto de Fisiolog√≠a y Biof√≠sica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiolog√≠a y Biof√≠sica Bernardo Houssay; ArgentinaFil: Luchelli, Luciana. Consejo Nacional de Investigaciones Cient√≠ficas y T√©cnicas. Oficina de Coordinaci√≥n Administrativa Houssay. Instituto de Fisiolog√≠a y Biof√≠sica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiolog√≠a y Biof√≠sica Bernardo Houssay; ArgentinaFil: Vila, Antonella. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnolog√≠a y Biolog√≠a Traslacional; ArgentinaFil: Blaustein, Mat√≠as. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnolog√≠a y Biolog√≠a Traslacional; ArgentinaFil: M√ľller Igaz, Lionel Ivan. Consejo Nacional de Investigaciones Cient√≠ficas y T√©cnicas. Oficina de Coordinaci√≥n Administrativa Houssay. Instituto de Fisiolog√≠a y Biof√≠sica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiolog√≠a y Biof√≠sica Bernardo Houssay; Argentin

    Synthesis of HA/beta-TCP bioceramic foams from natural products

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    A novel technology for the preparation of bioceramic foams (BF) using a simple and economic procedure is presented. This technology combines two conventional methods to produce a tridimensional macroporous structure by using a sol-gel route, submitted afterward to a microwave treatment and thermal annealing. The use of agri-waste products on this procedure, such as egg shell and white egg recycle, represents an interesting way for waste management while developing potential commercial biomaterials. The use of egg shell as eco-compatible reactant instead of commercial ones and the egg white as foaming agent to produce a tridimensional macroporous structures has been optimized by using a sol-gel route. The crystalline phase and quantitative phase composition has been studied by Rietveld refinement and the optimization of the foaming process and determination of interconnected porosity by scanning electron microscopy, Hg porosimetry and X-ray micro-CT imaging. Our results show that BF samples showed a composition of 60 wt% HA (hydroxyapatite) and 40 wt% beta-TCP (beta-tricalcium phosphate) with a total porosity of approx. 70 % and a porosity ranging from 5 to 300 mu m. These features indicate that BF samples are ideal for bone regeneration, and they are produced in an easy and environmental friendly viable process

    Results on Proton-Irradiated 3D Pixel Sensors Interconnected to RD53A Readout ASIC

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    Test beam results obtained with 3D pixel sensors bump-bonded to the RD53A prototype readout ASIC are reported. Sensors from FBK (Italy) and IMB-CNM (Spain) have been tested before and after proton-irradiation to an equivalent fluence of about 11 √ó\times 101610^{16} neq\text{n}_{\text{eq}} cm‚ąí2^{-2} (1 MeV equivalent neutrons). This is the first time that one single collecting electrode fine pitch 3D sensors are irradiated up to such fluence bump-bonded to a fine pitch ASIC. The preliminary analysis of the collected data shows no degradation on the hit detection efficiencies of the tested sensors after high energy proton irradiation, demonstrating the excellent radiation tolerance of the 3D pixel sensors. Thus, they will be excellent candidates for the extreme radiation environment at the innermost layers of the HL-LHC experiments.Comment: Conference Proceedings of VCI2019, 15th Vienna Conference of Instrumentation, February 18-22, 2019, Vienna, Austria. arXiv admin note: text overlap with arXiv:1903.0196

    Forward tracking at the next \boldmath{e+e‚ąíe^+e^-} collider Part II: experimental challenges and detector design

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    We present the second in a series of studies into the forward tracking system for a future linear e+e‚ąí e^+ e^- collider with a center-of-mass energy in the range from 250 GeV to 3 TeV. In this note a number of specific challenges are investigated, that have caused a degradation of the tracking and vertexing performance in the forward region in previous experiments. We perform a quantitative analysis of the dependence of the tracking performance on detector design parameters and identify several ways to mitigate the performance loss for charged particles emitted at shallow angle

    Propuesta de mejora del presupuesto participativo, de la Municipalidad Distrital de la Unión Leticia, 2021 - 2024

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    El presente Proyecto de Investigaci√≥n Aplicada - PIA, tiene como prop√≥sito contribuir a la calidad de vida de la poblaci√≥n en general, a trav√©s del, PROPUESTA DE MEJORA DEL PP DE LA MUNICIPALIDAD DISTRITAL DE LA UNION LETICIA, 2021 - 2024. Que comprende a (Educaci√≥n, Salud, Seguridad Alimentaria, Servicios P√ļblicos, Vivienda, y Seguridad Ciudadana), para la eficiente distribuci√≥n de los recursos asignados y el desarrollo sostenible. Para el cumplimiento y atenci√≥n de las necesidades b√°sicas, va a depender mucho de la correcta formulaci√≥n y desarrollo de los instrumentos de gesti√≥n que implementa el gobierno local, de entre ellos de manera particular el Proceso del PP, donde se tiene que mejorar la metodolog√≠a con el que se vino desarrollando a la fecha, espec√≠ficamente en la etapa de Identificaci√≥n y Priorizaci√≥n de Resultados y Evaluaci√≥n T√©cnica del Proyecto, la primera etapa sustentada como un proceso innecesario dado que este proceso se lleva a cabo en el desarrollo de los talleres del PDC, y al realizar un diagn√≥stico en cada Proceso del PP l√≥gicamente estar√≠a mostrando un resultado diferente dado por el escenario y actores diferentes y en la segunda etapa sustentado en que este proceso desarticula el cumplimiento de la programaci√≥n multianual que se tiene en el PDC, al priorizar proyectos que fueron identificados en los talleres del Proceso del PP el PDC siendo un instrumento matriz de gesti√≥n, estar√≠a quedando desplazado a consecuencia de los mecanismos que se viene empleando

    Development of a Tabletop Setup for the Transient Current Technique Using Two-Photon Absorption in Silicon Particle Detectors

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    The transient current technique (TCT) is widely used in the field of silicon particle detector development. So far, only laser wavelengths with a photon energy larger than or similar to the silicon bandgap (single photon absorption) were used. Recently, measurements using two-photon absorption (TPA) for silicon detector testing have been carried out for the first time. Excess carriers are only created at the focal point of the laser beam and thus resolution in all three spatial directions could be achieved. The resolution perpendicular to the incident laser beam could be increased roughly by a factor of 10. First measurements using this new method were performed at the Singular Laser Facility of Universidad del Pais Vasco (UPV)/Euskal Herriko Unibertzitatea (EHU). Following the initial success of the method, a compact TPA-TCT setup is under development. A first description of the setup and laser system is presented in this articleThis work was supported in part by the Spanish Ministry of Economy and Competitiveness (MINECO) under Grant FPA2013-48387-C6-1-P and in part by the Wolfgang Gentner Programme of the German Federal Ministry of Education and Research under Grant 05E15CH
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