28 research outputs found

    VP1-specific competition of polyomavirus seroresponses.

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    <p>Serial dilutions of four serum samples were preincubated with soluble recombinant fusion protein lysate containing GST.tag alone (black), GST-HPyV6 VP1.tag (orange), GST-HPyV7 VP1.tag (blue) or GST-TSPyV, GST-MCPyV, GST-BKPyV, GST-HPyV9 (grey). The HPyV6 (left part of the figure) and HPyV7 (right part of the figure) VP1 seroreactivity was measured by the Bio-Plex 100 analyzer. MFI stands for median fluorescent intensity.</p

    Age-specific seroreactivity against MCPyV, HPyV6, HPyV7, TSPyV, HPyV9 and BKPyV.

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    <p>The age-specific seroreactivity is measured amongst the respective seropositive individuals. The horizontal line in each boxplot represents the median MFI value. The box includes the 25%–75% MFI value range and the whiskers the 5%–95% MFI value range. Outliers are represented as a circle and extreme outliers as an asterisk.</p

    VP1 sequence identities and seroresponse correlations between tested polyomaviruses.

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    <p>The lower-left triangle shows homology (percentage sequence identity; 1 corresponds to 100% identity) between VP1 protein sequences of the six polyomaviruses tested generated by ClustalW alignment using Geneious Pro 5.6.3 software with default settings. For this alignment the following RefSeq/GenBank accession numbers were used: MCPyV, NC_010227; HPyV6, NC_014406; HPyV7, NC_014407; TSPyV, NC_014361; HPyV9, NC_015150; BKPyV, NC_001538. The upper-right triangle shows cross-reactivity between seroresponses measured against VP1 of the six polyomaviruses tested. For this analysis the serologic data from the complete study population were used (n = 799). Pearson correlation coefficients (r<sup>2</sup>) were determined by SPSS 20 software (IBM).</p

    Seropositivity of MCPyV, HPyV6, HPyV7, TSPyV, HPyV9 and BKPyV.

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    <p>Seropositivity is measured for the total study population divided into 14 different age groups. Error bars indicate 95% confidence intervals.</p

    Seroresponses measured against VP1 of MCPyV, HPyV6, HPyV7, TSPyV, HPyV9 and BKPyV.

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    <p>Presented are seroresponses of 799 Australian immunocompetent individuals. Each circle corresponds to the mean fluorecence intensity (MFI) measured for an individual serum sample in the Bio-Plex 100 analyzer. The cut-off value calculated for each polyomavirus as explained in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081078#pone.0081078.s001" target="_blank">Figure S1</a></b> is depicted as a blue line.</p

    Toscana virus tree showing H.sapiens-wt/Italy/2014/Genotype-A_BB8 segments detected in a returned Australian traveller

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    Toscana virus (TOSV) is identified in sandflies, animals and humans around the Mediterranean Sea. TOSV has not been reported in Australia. During investigations of cerebrospinal fluid samples from patients with encephalitis, a TOSV was identified in a traveller returning to Australia from Europe. <br>TOSV should be considered, especially during May to October, in travellers to Australia who embarked in countries in and around the Mediterranean Sea and who subsequently present for medical care because of neurological symptoms.<br><div><p>LEGEND.</p><p>Phylogenetic representation of complete and near complete Toscana virus (TOSV) lineage L (1a), M (1b) and S (1c) gene nucleotide segments compared to a Sandfly fever Naples virus (SFNV). Lineage A and B strains are indicated by blue (solid outline) and green boxes (dashed outline), respectively. A circle indicates the TOSV strain H.sapiens-wt/Italy/2014/Genotype-A_BB8, described here. MEGA7 was used to construct Neighbor-Joining trees with distances calculated using the p-distance method (500 bootstraps; percentage replicate tree branching patterns are shown next to initial branches). </p></div

    HRV-QPM detections and association of co-morbidities and severity of respiratory illness upon discharge.

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    <p>HMPV-Human metapneumovirus, HCoV-229E or -NL63-Human coronaviruses, HBoV-Human bocavirus, WUPyV-WU polyomavirus. Labs-Myco/Pert- laboratory testing for <i>Mycoplasma spp.</i> or <i>Bordetella pertussis.</i><sup>1</sup>Steroids, <sup>2</sup>adrenalin or <sup>3</sup>bronchodilators administered, <sup>4</sup>Comparison of severity score using Wilcoxon rank-sum test for HRV-QPM cases with and without co-detection, p = 0.88. <sup>5</sup>Recorded as ‘zero’ due to the patient being admitted for cardiac surgery and all criteria were in relation to the surgery and not the ARTI. <sup>*</sup> Not admitted. CF-cystic fibrosis. NC-No chart could be obtained for review, CHD-congenital heart disease. NN-No notes were available from presentation during the preliminary study.</p

    Predicted HRV-QPM pentamers compared to representative major (HRV-14) and minor (HRV-2) group HRV pentamers derived from crystallography data.

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    <p>(A) HRV-QPM versus HRV-14 SimPlot data projected onto a space filling depiction of the predicted HRV-QPM pentamer. Shading represents the amino acid identity (26–69%). The yellow dashed triangle represents a single icosahedral asymmetric unit (T = p3 conformation) composed of VP1 and VP2 from the same protomer and VP3 for an adjacent protomer. The major group domains of interest are divided between two asymmetric units for ease of viewing. Receptor (white) and antigenic (red) sites are shown in outline. (B) Top view ribbon depiction of a major group HRV pentamer (HRV-14; gray) with labelled antigenic neutralisation sites (NImIA-III, green) and combined HRV A (HRV-16) and B (HRV-14)ICAM-1 receptor footprints (red) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001847#pone.0001847-Laine1" target="_blank">[6]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001847#pone.0001847-Lole1" target="_blank">[37]</a>. Magnified areas of interest (boxed) highlight computer-based predictive comparisons to the equivalent HRV-QPM (orange) structures of interest. Arrows indicate structures and corresponding sequences of interest (refer to text). (C) HRV-QPM versus HRV-2 SimPlot data projected onto the HRV-QPM pentamer. The domains of interest are mostly shown within a single asymmetric unit. (D) A minor group pentamer (HRV-2; gray) including antigenic sites (sites A–C, green) and VLDL-R footprint (red) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001847#pone.0001847-Hofer1" target="_blank">[10]</a>. Attachment of the VLDL-R involves adjacent VP1 molecules. Magnified VP1 area represents one half of a VLDL-R footprint <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001847#pone.0001847-Stanway1" target="_blank">[42]</a>. Amino acid substitutions (arrowed) contributed to the differences between minor group sites B and C.</p
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