6 research outputs found

    Role of CXCL16 in dynamic adhesion erythrocytes to anti-CXCl16-treated endothelial cells under arterial shear stress. A.

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    <p>Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow. The erythorcytes were pretreated for 30 minutes with Ringer solution without (control) or with 1 µM ionomycin. The HUVEC were left untreated or treated for 2 hours with neutralizing antibody directed against endothelial CXCL16 (4 µg/ml), ***(p<0.001) indicates statistically significant difference from absence of ionomycin (1 µM), ###(p<0.001) indicates statistically significant difference from anti CXCL 16 (ANOVA). <b>B.</b> Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow. The erythrocytes were pretreated for 12 hours with Ringer with (control), or without (-Glucose) glucose. The HUVEC were left untreated or treated for 2 hours with neutralizing antibody directed against endothelial CXCL16 (4 µg/ml), ***(p<0.001) indicates statistically significant difference from absence of glucose, ###(p<0.001) indicates statistically significant difference from anti CXCL 16 (ANOVA). C. Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow. The erythrocytes were pretreated for 2 hours with isotonic (control) or hypertonic (550 mM sucrose) Ringer. The HUVEC were left untreated or treated for 2 hours with neutralizing antibody directed against endothelial CXCL16 (4 µg/ml), ***(p<0.001) indicates statistically significant difference from absence of hyperosmotic shock, ###(p<0.001) indicates statistically significant difference from anti CXCL 16 (ANOVA).</p

    Enhanced ionomycin induced eryptosis and adhesion of erythrocytes from annexin7-deficient mice. A.

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    <p>Histogram of annexin V-binding reflecting phosphatidylserine exposure in a representative experiment of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>) and their wild type control mice (<i>anx7<sup>+/+</sup></i>) exposed for 30 min to Ca<sup>2+</sup> ionophore ionomycin (1 µM). <b>B.</b> Arithmetic means ± SEM (n = 8−9) of the percentage of annexin V-binding erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) exposed for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant (p<0.001) difference from absence of ionomycin, *** significant difference (p<0.001) from <i>anx7<sup>+/+</sup></i> erythrocytes (ANOVA). <b>C.</b> Arithmetic means ± SEM (n = 8−9) of the forward scatter of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) exposed for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant (p<0.001) difference from absence of ionomycin, *** significant difference (p<0.001) from <i>anx7<sup>+/+</sup></i> erythrocytes (ANOVA). <b>D.</b> Arithmetic means ± SEM (n = 6) of the number of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) adhering to the human umbilical vein endothelial cells (HUVEC) following exposure for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant difference (p<0.001) from absence of ionomycin, *** significant difference (p<0.001) from <i>anx7<sup>+/+</sup></i> erythrocytes (ANOVA).</p

    Role of phosphatidylserine exposure in dynamic adhesion of annexin-treated erythrocytes to endothelial cells under arterial shear stress. A.

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    <p>Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow following exposure for 30 minutes to Ringer solution without (control) or with 1 µM ionomycin without or with a prior 30 min treatment with Annexin V (5 µl/ml). ***(p<0.001) indicates statistically significant difference from absence of ionomycin, ###(p<0.001) indicates statistically significant difference from absence of annexin V (ANOVA). <b>B.</b> Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow following a 12 hours treatment with Ringer solution with (control), or without (-Glucose) glucose without or with a prior 30 min treatment with Annexin V (5 µl/ml). ***(p<0.001) indicates statistically significant difference from absence of glucose, ###(p<0.001) indicates statistically significant difference from absence of annexin V (ANOVA). C. Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow following a 2 hours exposure to isotonic (control) or hypertonic (550 mM sucrose) Ringer solution without or with a prior 30 min treatment with Annexin V (5 µl/ml). ***(p<0.001) indicates statistically significant difference from absence of hyperosmotic shock, ###(p<0.001) indicates statistically significant difference from absence of annexin V (ANOVA).</p

    Correlation between phosphatidylserine exposure and dynamic adhesion of erythrocytes to endothelial cells under arterial shear stress.

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    <p>Arithmetic means ± SEM (n = 6−8) of the erythrocytes adhering to HUVEC as a function of the percentage erythrocytes binding annexin V. The erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, closed symbols) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, open symbols) were left without pretreatment (circles), or pretreated 30 minutes with 1 µM ionomycin (triangles), 12 hours with glucose-depleted Ringer (squares) or 2 hours with hyperosmotic shock by addition of 550 mM sucrose (diamonds).</p

    Enhanced eryptosis and adhesion of erythrocytes from annexin7-deficient mice following osmotic shock. A.

    No full text
    <p>Histogram of annexin V-binding reflecting phosphatidylserine exposure in a representative experiment of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>) and their wild type control mice (<i>anx7<sup>+/+</sup></i>) exposed for 2 hours to hyperosmotic shock (550 mM sucrose added). <b>B.</b> Arithmetic means ± SEM (n = 8−9) of the percentage of annexin V-binding erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) exposed for 2 hours to isotonic (left bars) or hyperosmotic (550 mM sucrose added, right bars) Ringer. ### significant (p<0.001) difference from isotonic Ringer, *** significant difference (p<0.001) from <i>anx7<sup>+/+</sup></i> erythrocytes (ANOVA). <b>C.</b> Arithmetic means ± SEM (n = 8−9) of the forward scatter of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) exposed for 2 hours to isotonic (left bars) or hyperosmotic (550 mM sucrose added, right bars) Ringer. ### significant (p<0.001) difference from isotonic Ringer, *** significant difference (p<0.001) from <i>anx7<sup>+/+</sup></i> erythrocytes (ANOVA). <b>D.</b> Arithmetic means ± SEM (n = 6) of the number of erythrocytes from annexin7-deficient mice (<i>anx7<sup>−/−</sup></i>, black bars) and their wild type control mice (<i>anx7<sup>+/+</sup></i>, white bars) adhering to human umbilical vein endothelial cells (HUVEC) following exposure of the erythrocytes for 2 hours to isotonic (left bars) or hyperosmotic (550 mM sucrose added, right bars) Ringer. ### significant (p<0.001) difference from isotonic Ringer, *** significant difference (p<0.001) from <i>anx7<sup>+/+</sup></i> erythrocytes (ANOVA).</p

    Annexin-binding erythrocytes in ischemic kidneys from wild type and from annexin deficient mice. A.

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    <p>Arithmetic means ± SEM (n = 7) of erythrocyte abundance in the inner stripe of renal tissue following renal ischemia/reperfusion of anx7<sup>−/−</sup> mice (black bar) and <i>anx7<sup>+/+</sup></i> mice (white bar). <b>B.</b> Arithmetic means ± SEM (n = 7) of the acute tubular necrosis (ATN) score in the inner stripe of renal tissue following renal ischemia of anx7<sup>−/−</sup> mice and <i>anx7<sup>+/+</sup></i> mice, ***(p<0.001) indicate significant difference to wild type mice (ANOVA). <b>C.</b> Arithmetic means ± SEM (n = 7) of the acute tubular necrosis (ATN) score in the outer cortex of renal tissue following renal ischemia of anx7<sup>−/−</sup> mice and <i>anx7<sup>+/+</sup></i> mice, ***(p<0.001) indicate significant difference to wild type mice (ANOVA). <b>D, E.</b> Representative light microscopy image from kidney section of ischemic <i>anx7<sup>+/+</sup></i> mice (<b>D</b>) and anx7<sup>−/−</sup> mice (<b>E</b>) stained with sirius red. Arrows indicate yellow stained erythrocytes within renal capillaries. <b>F, G.</b> Representative light microscopy image from kidney section of ischemic <i>anx7<sup>+/+</sup></i> mice (<b>F</b>) and anx7<sup>−/−</sup> mice (<b>G</b>) stained with PAS.</p
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