31 research outputs found

    Automated production of recombinant human proteins as resource for proteome research-1

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    Cation strategy. Protein expression was induced at 25°C (white), 30°C (dark grey) and 37°C (light grey), respectively.<p><b>Copyright information:</b></p><p>Taken from "Automated production of recombinant human proteins as resource for proteome research"</p><p>http://www.proteomesci.com/content/6/1/4</p><p>Proteome Science 2008;6():4-4.</p><p>Published online 28 Jan 2008</p><p>PMCID:PMC2266735.</p><p></p

    Automated production of recombinant human proteins as resource for proteome research-2

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    Usion proteins. (B) 96 samples can be loaded on a single E-PAGE gel comprising twelve lanes in eight rows (A-H). A single additional lane is available per row to accommodate a molecular weight standard. (C) Single lanes (each 2 cm in length) are assembled to an artificial gel image to facilitate sample analysis. (D) Example molecular weight marker separated by the E-PAGE system.<p><b>Copyright information:</b></p><p>Taken from "Automated production of recombinant human proteins as resource for proteome research"</p><p>http://www.proteomesci.com/content/6/1/4</p><p>Proteome Science 2008;6():4-4.</p><p>Published online 28 Jan 2008</p><p>PMCID:PMC2266735.</p><p></p

    Extending pathways based on gene lists using InterPro domain signatures-1

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    <p><b>Copyright information:</b></p><p>Taken from "Extending pathways based on gene lists using InterPro domain signatures"</p><p>http://www.biomedcentral.com/1471-2105/9/3</p><p>BMC Bioinformatics 2008;9():3-3.</p><p>Published online 4 Jan 2008</p><p>PMCID:PMC2245903.</p><p></p> form of random genes that are not assigned to the respective pathways. The number of noise genes was fixed to 50. The similiarity measures and graphs are produced similar as for Figure 2. a) single pathway b) all 181 KEGG pathways

    Alpinia flabellata Ridl.

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    原著和名: イリオモテクマタケラン科名: ショウガ科 = Zingiberaceae採集地: 沖縄県 西表島 祖納〜白浜 (琉球 西表島 祖納〜白浜)採集日: 1986/12/7採集者: 萩庭丈壽整理番号: JH015467国立科学博物館整理番号: TNS-VS-96546

    AGE accumulation under exogenous aldehyde stress.

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    <p>A: AGE accumulation (CML (left) and MG-AGE (right)) in MCF-7-Md (lower panel) and TamR-Md cells (upper panel) after cultivation for three days with different concentrations of methylglyoxal and glyoxal as shown by Western blotting. Strongly enhanced AGE accumulation became visible when cell number was significantly reduced due to toxic effects, as represented by the decreasing β-actin signal. Glyoxal resulted in accumulation of CML whereas methylglyoxal treatment caused MG-AGE modification. Cells were treated as described for the determination of vitality/proliferation. Proteins were extracted per well and not corrected for protein amount. Therefore, the blots represent adherent cells only. B) AGE accumulation shown by immunofluorescence. Composite images of dual exposures are shown. MCF-7-Md cells were stained for CML and MG-AGE (red) by specific antibodies as indicated and nuclear staining was achieved with DAPI (blue) of cells treated for 24 h with 1 mM methylglyoxal or glyoxal.</p

    Phosphorylation of IκBα in response dicarbonyl stress.

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    <p>Cells were incubated with different concentrations of dicarbonyls and with Il-1β (10 ng/mL) as positive control treatment for 10 minutes. IκBα phosphorylation was detected by Western blotting. Experiment has been performed three times with duplicates for each treatment, one representative blot is shown.</p

    Kinase phosphorylation in MCF-7-Md and TamR-Md cells in response to glyoxal and methylglyoxal.

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    <p>Confluent and serum starved cells were incubated with different concentrations of dicarbonyls for 10 minutes before proteins were extracted and subjected to Western blot analysis. Experiments were repeated three times in duplicate. A representative Western blot and the quantification of the signals of all experiments are shown. Band intensity was expressed relative to the β-actin signal and control treatments were set to 1 for each cell-line.</p
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