6,930 research outputs found

    amsrpm: Robust Point Matching for Retention Time Aligment of LC/MS Data with R

    Get PDF
    Proteomics is the study of the abundance, function and dynamics of all proteins present in a living organism, and mass spectrometry (MS) has become its most important tool due to its unmatched sensitivity, resolution and potential for high-throughput experimentation. A frequently used variant of mass spectrometry is coupled with liquid chromatography (LC) and is denoted as "LC/MS". It produces two-dimensional raw data, where significant distortions along one of the dimensions can occur between different runs on the same instrument, and between instruments. A compensation of these distortions is required to allow for comparisons between and inference based on different experiments. This article introduces the amsrpm software package. It implements a variant of the Robust Point Matching (RPM) algorithm that is tailored for the alignment of LC and LC/MS experiments. Problem-specific enhancements include a specialized dissimilarity measure, and means to enforce smoothness and monotonicity of the estimated transformation function. The algorithm does not rely on pre-specified landmarks, it is insensitive towards outliers and capable of modeling nonlinear distortions. Its usefulness is demonstrated using both simulated and experimental data. The software is available as an open source package for the statistical programming language R.

    The development of a mapping tool for the evaluation of building systems for future climate scenarios on European scale

    Full text link
    The paper presents a tool for the mapping of the performance of building systems on European scale for different (future) time periods. The tool is to use for users and be applicable for different building systems. Users should also be able to use a broad range of climate parameters to assess the influence of climate change on these climatic parameters. Also should the calculation time be reasonable short. The mapping tool is developed in MATLAB, which can be used by other users for their own studies.Comment: 21 pages, 24 figures, pre-conferenc

    Alien Registration- Steen, Hans A. (Portland, Cumberland County)

    Get PDF