10 research outputs found

    Digital Pathology for Analyzing Endomyocardial Biopsy Specimens

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    Introduction: Visual assessment of endomyocardial biopsy (EMB) specimens may result in errors due to the human factor and inability to obtain quantitative data on the intensity of immunohistochemical (IHC) reactions and severity of pathological changes in the heart transplant. Detailed digital characterization of EMB specimens based on automated or semi-automated computer-assisted morphometry improves the diagnostic accuracy of EMB.   Objective: To develop a method of and algorithms for digital computer-assisted analysis of EMB specimens to quantify pathomorphological and immunophenotypic changes in the myocardium of patients with various types and grades of heart transplant rejection.   Materials and methods: We studied 257 EMB specimens from 56 heart transplant recipients at Scientific Research Institute – Ochapovsky Regional Clinical Hospital No. 1 (Krasnodar, Russian Federation). Sections were stained with hematoxylin and eosin. We used the streptavidin-biotin method to determine the CD68 expression and computer-assisted morphometry of digital images to measure the area of pathological changes and CD68 expression.   Results: When developing a method for computer-assisted analysis of EMB specimens, we proposed a new evaluation criterion: staining area coefficient (%) that is a ratio of the total stained area to the total section area. We created and field-tested algorithms for digital morphometric analysis to assess the intensity of IHC reactions and to identify the relative area of pathological changes in the EBM specimens.   Conclusions: New digital criteria for diagnosing heart transplant rejections are to improve the accuracy of EMB results interpretation, but can also be used for analyzing other biopsy specimens

    The effect of the composition of Soderm-Forte gel and the new injectable form of Rexod on pathology findings in gingival tissue in experimental periodontitis in rats

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    The combination of TDT, Soderm-Forte gel and the NIF of Rexod shows the most beneficial effect on the pathological processes in the gum. The pharmacotherapeutic effect of the studied combination promotes the earliest regeneration of damaged gum tissues and reduces the risk of persistent pathology changes in the

    Pathomorphological analysis of the gum tissues in the application of the combination of Soderm®- Forte gel with Cytoflavin® in experimental periodontitis in rats

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    The combination of TDT, Soderm®-Forte and Cytoflavin® in EP has a more pronounced therapeutic effect, manifested by early regression of pathological changes and acceleration of tissue regeneration in the gu

    The effect of zinc complex of N-isopropenylimidazole on the morphological characteristics of gum tissues in experimental endodontic-periodontal lesions in rats Authors

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    Introduction: Combined inflammatory and destructive processes affecting the dental pulp and tissues of the periodontal complex are among the most problem diseases of the dental system. Current therapy with use of available pharmacological agents does not always allow achieving the expected positive result. In addition, often the lack of information about morphological processes in the tissues of the dental system, in particular the gums, with endodontic-periodontal lesion (EPL) limits the ability of dentists to carry out targeted pharmacotherapy with both traditional and, in particular, new medications. The aim of the study was to evaluate the morphological characteristics of gum tissues in a therapeutic context of N-isopropenylimidazole zinc complex derivative in experimental endodontic-periodontal lesion in rats. Materials and Methods: A simulation of EPL in rats was performed in two ways: simultaneous induction of acute periodontitis and parodontitis by pulp extraction and natural infection of the pulp cavity, as well as by ligation of the necks of lower incisors. The research protocols included 5 groups of animals: 1st – intact group (control-1); 2nd – animals with simulated EPL (control-2); 3rd – animals with simulated EPL and treated with Metrogyl Denta® gel (M-D); 4th – animals with simulated EPL and treated with N–isopropenylimidazole zinc complex derivative gel under the laboratory code Pilim-1; and 5th – animals with simulated EPL and treated with the combination of M-D + Pilim-1. The gum of the lower incisors was taken for morphological studies. Slides were stained with hematoxylin and eosin. Computer morphometry was performed using the ImageJ software. Results and Discussion: The substances M-D, Pilim-1 and, especially, the combination of M-D+Pilim-1 (against the background of chlorhexidine bigluconate used as oral rinse) for 14 days in rats with simulated EPL cause a significant improvement of the morphological structure of the gum with minimal residual dystrophy and sclerosis. The combination M-D + Pilim-1 led to a 1.3-time increase in epithelial thickness, and a 1.5-time decrease in acanthosis depth in comparison with M-D, while the number of capillaries and their diameter had no significant differences. Compared with Pilim-1, the epithelial thickness increased 1.5 times, and the acanthosis depth and the number of capillaries decreased 1.6 and 1.4 times, respectively, whereas the diameter of the capillaries did not change significantly. The pronounced protective effect of the combination M-D + Pilim-1 on the morphological structure of the gingival mucosa of rats with simulated EPL may be associated with antimicrobial, anti-inflammatory, regenerating, angioprotective and antioxidant properties of both M-D and Pilim-1 separately, and, possibly to a greater extent, of the combination M-D + Pilim-1. Conclusion: The substances M-D, Pilim-1 and, especially, the combination M-D + Pilim-1 (against the background of chlorhexidine bigluconate used as oral rinse) for 14 days in rats with simulated EPL have a protective effect on the epithelial structure and the connective tissue of the proper mucous plate, manifested in active normalization of pathological changes and significant restoration of their organotypic structure

    EXPRESSION OF CELL ADHESION MOLECULES AND COMPLEMENT RECEPTORS IN DIAGNOSTICS OF TRANSPLANTED HEART REJECTION

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    Aim. Characterization of the changes in expression of cell adhesion molecules and complement receptors in the transplanted heart rejection using computer morphometry to improve the quality of endomyocardial biopsy diagnostics.Materials and methods. Endomyocardial biopsies of 20 patients undergoing cardiac transplantation were used. Histological sections were stained using a standard procedure with hematoxylin and eosin, as well as an indirect immunohistochemical method with the ABC detection system against CD21 and CD31. The area of the positive reaction was estimated with computer morphometry.Results. All biopsies are divided according to the degrees of rejection as follows: degree 0R – 8 samples, 1R – 10 samples, 2R – 7 samples, 3R – 4 samples. CD21 expression is 0.31% in samples with 0R, 1.09% at 1R, 2R rejection of 2.01%, and for 3R – 4.15%. The area occupied by CD31-positive cells is 3.49% in 0R, at 1R rejection this index decreases by 1.3 times, in biopsy samples with 2R this index is 1.8 times less, and for samples with 3R it decreases by 2.6 times.Conclusion. Evaluation of CD21-positive cells in the myocardium allows us to predict cardiosclerosis, as well as a tendency to a chronic clinical course of the rejection. Expression of CD31 makes it possible to assess condition of the microcirculatory vessels in the graft, which is also important for the heart failure prevention

    MORPHOLOGICAL INDICATORS OF MYOCARDIAL DAMAGES IN REJECTION OF TRANSPLANTED HEART

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    Aim. Characterization of histological indicators of myocardial damage in rejection of transplanted heart using computer morphometry to improve the quality of biopsy diagnostics. Materials and methods. Endomyocardial biopsies of 31 patients after cardiac transplantation were used. Histological sections were stained using the standard procedure with hematoxylin and eosin, and also with picro-fuchsin by van Gieson. The area of the biopsy sample, fibrosis, necrosis, lipomatosis and hemorrhages, as well as the number of cells in the infiltrate, was assessed using computer morphometry. Results. All biopsies are divided according to the degrees of rejection as follows: degree 0R – 16 samples, 1R – 14 samples, 2R – 10 samples, 3R – 4 samples. The severity of necrosis significantly increased along the increase in rejection severity, reaching 0% at 0R, 0.03% at 1R, 4.95% at 2R and 17.4% at 3R. The most massive hemorrhages were detected in samples attributed to 2R – 9.89% of the biopsy area. Lipomatosis was noted at all levels of rejection, reaching a maximum of 16.5% of the area in patients with 3R. The area of fibrosis increased with an increase in the severity of rejection: at zero degree it was 0.16% of the biopsy, at the first – 3.8%, at the second – 8.69%. Conclusion. The studied morphological indicators make possible a more detailed assessment of the degree, as well as the course of the rejection reaction, which will improve the quality of diagnosis with endomyocardial biopsy

    The effect of the composition of Soderm®-Forte gel and the new injectable form of Rexod® on pathology findings in gingival tissue in experimental periodontitis in rats

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    Introduction: Periodontitis is the most important problem of modern dentistry. The development of new medicines and treatment regimens for patients with periodontal complex lesions is a strategic direction of modern pharmacology and dentistry. In this view, pride of place goes to morphological research, which allows not only to study the effect of drugs on pathomorphological changes in periodontal tissues, but also to estimate their therapeutic effectiveness. Aim of the study: to determine the nature of the effect of the composition of Soderm®-Forte gel and the new injectable form of Rexod® on the pathology findings in gingival tissue of rats with experimental periodontitis. Materials and methods: Experimental periodontitis (EP) was induced in rats by ligature method. The study was performed according to the following algorithm: animals with intact periodontium; animals with untreated EP; animals with EP treated with traditional drug therapy (TDT); animals with EP treated with combinations of TDT with Soderm®-Forte gel and TDT with Soderm®-Forte gel and the new injectable form (NIF) of Rexod®. For pathomorphological examination, biopsy specimen was taken from the gingival margin of the lower incisors. The ImageJ software was used for computer morphometry. Results and discussion: Examination of the gum samples revealed moderate therapeutic effects of the TDT. The combinations of TDT with Soderm®-Forte gel and, to a greater extent, TDT with Soderm®-Forte gel and the NIF of Rexod® showed high pharmacotherapeutic efficacy, manifested in rapid regeneration of the gingival tissues. Conclusion: The combination of TDT, Soderm®-Forte gel and the NIF of Rexod® shows the most beneficial effect on the pathological processes in the gum. The pharmacotherapeutic effect of the studied combination promotes the earliest regeneration of damaged gum tissues and reduces the risk of persistent pathology changes in them
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