1,474 research outputs found

    Proteomics in the Light of Integral Value Transformations

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    In this paper, Proteomics have been studied in the light of Integral Value Transformations (IVTs) which was introduced by Sk. S. Hassan et al in 2010. For case study, a Human olfactory receptor OR1D2 protein sequence has been taken and then different IVTs have been used to evolve OR1D2 into some other proteomic like sequences. It has been observed that some of the generated sequences have been mapped to another olfactory receptor in Human or in some other species. Also it has been corroborated through fractal dimension that some of the fundamental protein properties have been nearly intact, even after the mapping. This study will help to comprehend the proteomic evolutionary network with the help of IVTs

    Activity Guided Isolation and Characterization of Antiplasmodial Agents of some Local medicinal Plants

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    Combined Plant Mixture (CPM) in ratio of 1:1 (Vitex doniana, Diospyros mesipiliformis, Acacia polyacantha, Parinari macrophylla, Ficus sycomorus and Parkia biglobosa) was extracted with methanol and screened for antimalarial activity. The CPM Methanol extract residue was suspended in water and fractionated with Hexane (HX), Ethylacetate (EA) and n-Butanol (BUT). The Methanolic CPM extract has shows some antimalarial activity with Mean Survival Times (MST) of 10.16 to 12.16 days. All the mice in control group were found dead on 14th day of study and one mice was still alive on 14th day in both the groups with 15% and 30% parasitaemia against Plasmodium yoelli nigeriensis N67 and Plasmodium berghei K173 respectively. The EA fraction was the most active against P. yoelli nigeriensis N67 having 2 mice surviving up to 14th day with 5.5% parasitaemia and MST of 13.4 days. Fractions of water (WT), HX and BUT have 12.5, 25, and 100% (dead) parasitaemia with mean survival times of 12.6, 9.33 and 8.5 days against 100% (dead) parasitaemia and 8.20 days of MST when compared with control. HX fraction was the most active against P. berghei K173 followed by BUT fraction with one mice surviving up to 14th day without parasitaemia but with MST of 15.0 and 13.5 days respectively. EA and WT fractions have 22.66 and 100% (dead) parasitaemia with mean survival times of 11.80 and 10.80 days respectively. Chromatographic separation of EA fraction of the CPM extract resulted in the isolation of Gallic acid (Figure 2, Fractions 79-83), 3β-OH-α-amyrin (Figure 3, Fractions 45-46), 5,7,3’,4’,5’- pentahydroxy-3-O-glucophyranoside flavones (Figure 4, Fractions 128-157)-[myricetin 3-O-β-rhamnopyranoside], 5,7,3’,4’ tetrahydroxy-3-o-glucopyranoside flavones(Figure 5, Fractions 126-141)-[quercetin 3-O-β-rhamnopyranoside], and 3,5,7,3’,4’-Pentahydroxy flavones (Figure 6, Fractions 87-112)-[quercetine]. The compounds were characterized using ESI-MS, IR, 1H 13C, HMBC/HSQC and COSY-NMR data. These findings suggest that the combined extracts possess antiplasmodial properties.Keywords: Antimalarial, combined plant mixture, 1H 13C NMR, activity guided fractionatio

    Understanding Genomic Evolution of Olfactory Receptors through Fractal and Mathematical Morphology

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    Fractals and Mathematical Morphology are immensely used to study many problems in different branches of science and technology including the domain of Biology. There are many more unrevealed facts and figures of genes and genome in Computational Biology. In this paper, our objective is to explore how the evolutionary network is associated among Human, Chimpanzee and Mouse with regards to their genomic information. We are about to explore their genomic evolution through the quantitative measures of fractals and morphology. We have considered olfactory receptors for our case study. These olfactory receptors do function in different species with subtle differences in the structures of DNA sequences. Those subtle differences can be exposed through intricate details of Fractals and Mathematical Morphology

    Capturing the Occult Central Retinal Artery Occlusion Using Optical Coherence Tomography

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    AIMS: To report spectral-domain optical coherence tomography (OCT) findings in cases of impending or occult central retinal artery occlusion (CRAO) in which a diagnosis other than CRAO was made on initial presentation. METHODS: Retrospective, observational case series of patients diagnosed with CRAO for whom on initial presentation fundal examination and OCT findings were deemed unremarkable and/or a diagnosis other than CRAO was made. OCT images from the initial presentation were then reviewed for evidence of inner retinal ischaemia. RESULTS: In total, 214 cases of CRAO were identified. Eleven patients (5.14%) had been given an alternative initial diagnosis at their first presentation in casualty and were included. The age range was 20–84 years and 81% (9/11) were male. On review of initial OCT imaging performed in casualty, all cases had evidence of inner retinal ischaemia. Conclusions: CRAO is an ophthalmic emergency which leads to vision loss which is often irreversible. Examination of the fundus may be normal early in the course of the disease and therefore a timely diagnosis may be missed. This case series reports the OCT findings of inner retinal ischaemia in patients with occult or impending CRAO which may aid in the early diagnosis and referral to stroke services

    Ramond-Ramond Cohomology and O(D,D) T-duality

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    In the name of supersymmetric double field theory, superstring effective actions can be reformulated into simple forms. They feature a pair of vielbeins corresponding to the same spacetime metric, and hence enjoy double local Lorentz symmetries. In a manifestly covariant manner --with regard to O(D,D) T-duality, diffeomorphism, B-field gauge symmetry and the pair of local Lorentz symmetries-- we incorporate R-R potentials into double field theory. We take them as a single object which is in a bi-fundamental spinorial representation of the double Lorentz groups. We identify cohomological structure relevant to the field strength. A priori, the R-R sector as well as all the fermions are O(D,D) singlet. Yet, gauge fixing the two vielbeins equal to each other modifies the O(D,D) transformation rule to call for a compensating local Lorentz rotation, such that the R-R potential may turn into an O(D,D) spinor and T-duality can flip the chirality exchanging type IIA and IIB supergravities.Comment: 1+37 pages, no figure; Structure reorganized, References added, To appear in JHEP. cf. Gong Show of Strings 2012 (http://wwwth.mpp.mpg.de/members/strings/strings2012/strings_files/program/Talks/Thursday/Gongshow/Lee.pdf

    Identification of Serotype in Culture Negative Pneumococcal Meningitis Using Sequential Multiplex PCR: Implication for Surveillance and Vaccine Design

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    BACKGROUND: PCR-based serotyping of Streptococcus pneumoniae has been proposed as a simpler approach than conventional methods, but has not been applied to strains in Asia where serotypes are diverse and different from other part of the world. Furthermore, PCR has not been used to determine serotype distribution in culture-negative meningitis cases. METHODOLOGY: Thirty six serotype-specific primers, 7 newly designed and 29 previously published, were arranged in 7 multiplex PCR sets, each in new hierarchies designed for overall serotype distribution in Bangladesh, and specifically for meningitis and non-meningitis isolates. Culture-negative CSF specimens were then tested directly for serotype-specific sequences using the meningitis-specific set of primers. PCR-based serotyping of 367 strains of 56 known serotypes showed 100% concordance with quellung reaction test. The first 7 multiplex reactions revealed the serotype of 40% of all, and 31% and 48% non-meningitis and meningitis isolates, respectively. By redesigning the multiplex scheme specifically for non-meningitis or meningitis, the quellung reaction of 43% and 48% of respective isolates could be identified. Direct examination of 127 culture-negative CSF specimens, using the meningitis-specific set of primers, yielded serotype for 51 additional cases. CONCLUSIONS: This PCR approach, could improve ascertainment of pneumococcal serotype distributions, especially for meningitis in settings with high prior use of antibiotics