77 research outputs found

    SV2_Enface.mp4

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    En face maximum intensity projection (400 μm slices) of fibroblast cell dynamics. Scale bar represents 50 μm for all sections

    SV1_Vol.mp4

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    Volumetric visualization of fibroblast cell dynamics. The central part has 1mm × 1mm × 1mm field of view, and all the extracted parts have 0.25mm × 0.25mm × 0.25mm field of view

    Supplemental Material, supporting_information - Synthesis and properties of bismaleimide resins containing phthalide cardo and cyano groups

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    <p>Supplemental Material, supporting_information for Synthesis and properties of bismaleimide resins containing phthalide cardo and cyano groups by Siyang Liu, Yuanying Wang, Ping Chen, Dongwei Xu, Xuhai Xiong, and Xiaoyu Yan in High Performance Polymers</p

    Biodegradable Polymer Nanoparticles for Photodynamic Therapy by Bioluminescence Resonance Energy Transfer

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    Conventional photodynamic therapy is severely constrained by the limited light-penetration depth in tissue. Here, we show efficient photodynamic therapy (PDT) mediated by bioluminescence resonance energy transfer (BRET) that overcomes the light-penetration limitation. The photosensitizer Rose Bengal (RB) was loaded in biodegradable poly­(lactic-<i>co</i>-glycolic acid) (PLGA) nanoparticles, which were then conjugated with firefly luciferase. Spectroscopic characterizations indicated that BRET effectively activated RB to generate reactive oxygen species (ROS). In vitro studies of the cellular cytotoxicity and photodynamic effect indicated that cancer cells were effectively destroyed by BRET-PDT treatment. In vivo studies in a tumor-bearing mouse model demonstrated that tumor growth was significantly inhibited by BRET-PDT in the absence of external light irradiation. The BRET-mediated phototherapy provides a promising approach to overcome the light-penetration limitation in photodynamic treatment of deep-seated tumors

    Near-Infrared Optical Transducer for Dynamic Imaging of Cerebrospinal Fluid Glucose in Brain Tumor

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    Aberrant cerebral glucose metabolism is related to many brain diseases, especially brain tumor. However, it remains challenging to measure the dynamic changes in cerebral glucose. Here, we developed a near-infrared (NIR) optical transducer to sensitively monitor the glucose variations in cerebrospinal fluid in vivo. The transducer consists of an oxygen-sensitive nanoparticle combined with glucose oxidase (GOx), yielding highly sensitive NIR phosphorescence in response to blood glucose change. We demonstrated long-term continuous glucose monitoring by using the NIR transducer. After subcutaneous implantation, the glucose transducer provides a strong luminescence signal that can continuously monitor blood glucose fluctuations for weeks. By using the NIR emission of the transducer, we further observed abnormal dynamic changes in cerebrospinal fluid glucose and quantitatively assessed cerebral glucose uptake rates in transgenic mice bearing brain tumors. This study provides a promising method for the diagnosis of various metabolic diseases with altered glucose metabolism

    16 significant mutations between the ART and the naïve-therapy population.

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    <p>Note:</p>a<p>R indicated mutations that could confer resistance to antiviral drugs, and</p>b<p>U indicated mutations which confers an unknown response to antiviral drugs.</p

    Lipidomic analysis of TGs by shotgun mass spectrometry of EWAT from <i>Bscl2<sup>+/+</sup></i> and <i>Bscl2<sup>−/−</sup></i> mice.

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    <p>TG species were determined using high resolution ESI-MS and confirmed via product ion scan mode CID-MS/MS and –MS<sup>3</sup> as described in Methods (n = 3 pooled from 6 animals). Data are expressed as % total TG ion abundance in each genotype. Only the 46 TG species observed at >0.1% total TG ion abundance in <i>Bscl2<sup>+/+</sup></i> EWAT are listed. Data are presented as means ± SD. *: p<0.05; **: p<0.005. Arrows indicate upregulation or downregulation vs. <i>Bscl<sup>+/+</sup></i> EWAT.</p

    Sequencing results of the constructed plasmid pNL4-3 with mutations.

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    <p>All sequences were obtained from a single colony, and sequencing results from the supernatant/harvest virions matched the sequence from the single colony, suggesting that the mutations were not altered during transfection and infection.</p

    Facile Ultrasonic Synthesis of CoO Quantum Dot/Graphene Nanosheet Composites with High Lithium Storage Capacity

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    In this paper, we report a facile ultrasonic method to synthesize well-dispersed CoO quantum dots (3–8 nm) on graphene nanosheets at room temperature by employing Co<sub>4</sub>(CO)<sub>12</sub> as cobalt precursor. The prepared CoO/graphene composites displayed high performance as an anode material for lithium-ion battery, such as high reversible lithium storage capacity (1592 mAh g<sup>–1</sup> after 50 cycles), high Coulombic efficiency (over 95%), excellent cycling stability, and high rate capability (1008 mAh g<sup>–1</sup> with a total retention of 77.6% after 50 cycles at a current density of 1000 mA g<sup>–1</sup>, dramatically increased from the initial 50 mA g<sup>–1</sup>). The extraordinary performance arises from the structure advantages of the composites: the nanosized CoO quantum dots with high dispersity on conductive graphene substrates supply not only large quantity of accessible active sites for lithium-ion insertion but also good conductivity and short diffusion length for lithium ions, which are beneficial for high capacity and rate capability. Meanwhile, the isolated CoO quantum dots anchored tightly on the graphene nanosheets can effectively circumvent the volume expansion/contraction associated with lithium insertion/extraction during discharge/charge processes, which is good for high capacity as well as cycling stability. Moreover, regarding the anomalous behavior of capacity increase with cycles (activation effect) observed, we proposed a tentative hypothesis stressing the competition between the conductivity increase and the amorphorization of the composite electrodes during cycling in determining the trends of the capacity, in the hope to gain a fuller understanding of the inner working of the novel nanostructured electrode-based lithium-ion batteries
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