172 research outputs found

    Lactobacillus crispatus represses vaginolysin expression by BV associated Gardnerella vaginalis and reduces cell cytotoxicity

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    Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis. This effect was associated to the expression of vaginolysin and was specific to L. crispatus interference, as other vaginal facultative anaerobes had no protective effect.This work was supported by Portuguese National Funds (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684). JC, and MER acknowledge the financial support of individual Grants SFRH/BD/93963/2013, and SFRH/BPD/95401/2013 respectively. NC is an Investigador FCT.info:eu-repo/semantics/publishedVersio

    Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents

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    Background: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, the ability of C. albicans biofilm to tolerate the novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially to the standard irrigation of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures of C. albicans SC 5314 were treated sequentially with NaOCl and EDTA and exposed to MEK/TCE or MEK/OOil. The effect of the antimicrobial treatment was evaluated using the disk diffusion method for planktonic culture, and the enumeration of colony-forming units (CFUs) and scanning electron microscope (SEM) for biofilm cell culture. Results: C. albicans mature biofilm (24 h) was significantly reduced in biomass and cell viability after solvent mixtures exposure, compared with the previous NaOCl and EDTA treatments. MEK/OOil combination caused a total reduction of biofilm, while with MEK/TCE, there was a 3-log (CFU/cm2) reduction compared with the sequence NaOCl and EDTA, and a 4-log (CFU/cm2) reduction compared with the control. Conclusions: The additional exposure of a preformed 24 h C. albicans biofilm to novel solvent mixtures MEK/TCE and MEK/OOil caused a positive antibiofilm impact, overcoming the performance of the conventional endodontic irrigating protocol.This article was supported by National Funds through FCT-Fundação para a Ciência e a Tecnologia, I.P., within CINTESIS, R&D Unit (reference UIDB/4255/2020). This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and grant ref 2020.05720.BD for Liliana Fernandes; BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020-Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersio

    Preliminary evaluation of microcarrier culture for growth and monoclonal antibody production of CHO-K1 cells

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    Large-scale biopharmaceutical production commonly relies on suspension cell cultures that provide higher yields than adherent cultures. However, most mammalian cells grow adherently and therefore need to be adapted to suspended growth, which is not always simple or feasible. Microcarrier culture introduces new possibilities and makes achievable the practical high yield culture of anchorage-dependent cells in suspension systems. The aim of this study was to evaluate and optimize the use of microcarrier culture for the growth and antibody production of CHO-K1 cells. For this, the macroporous Cultispher microcarriers were used, and the initial cell adhesion to the microcarriers (occurring in the first 5-6 hours) and further cell proliferation were assessed. Cultures of antibody-producing CHO-K1 cells were performed in 50 ml vented conical tubes, and different conditions were tested: initial cell concentration (2x105 cells/ml and 4x105 cells/ml), microcarrier concentration (1 g/L and 2 g/L), type of rocking during the first 6 hours of adhesion (pulse or continuous) and rocking after initial adhesion (no rocking and 60 rpm). Cell concentration and viability in the microcarriers were assessed periodically (hourly for the adhesion phase, and daily after that). It was observed that an increase in the initial cell concentration does not enhance initial adhesion, possibly due to saturation of the microcarrier surface. For its turn, increasing microcarrier concentration, without further increasing initial cell concentration does not improve cell densities achieved in the culture. Concerning rocking, the most favorable type for the adhesion phase was pulse rocking and, after this, a continuous rocking provided an improved cell proliferation. In conclusion, microcarrier cultures proved to be a viable alternative to suspended cultures for the growth and antibody production of CHO-K1 cells

    Reorganizando o Laboratório de Ciências: uma experiência da abordagem do PIBID/UESC de Química no espaço escolar

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    A forma√ß√£o de professores de Qu√≠mica tem sido discutida por diversos profissionais da educa√ß√£o que buscam, dentre outras coisas, apresentar os aspectos que est√£o envolvidos nesse processo. Sendo assim, o intuito deste trabalho √© apresentar os resultados obtidos com a interven√ß√£o do PIBID/Qu√≠mica UESC em que houve a reorganiza√ß√£o de um laborat√≥rio de Ci√™ncias e esta atividade foi relacionada √† forma√ß√£o dos licenciandos envolvidos. Pretendemos tamb√©m demonstrar a import√Ęncia deste espa√ßo para a forma√ß√£o de conceitos de Ci√™ncias, mas precisamente de Qu√≠mica. Para tal, executamos uma atividade em grupo onde a reorganiza√ß√£o deste laborat√≥rio foi o foco principal, al√©m da aplica√ß√£o de um question√°rio buscando conhecer as impress√Ķes dos alunos a cerca do local. Pudemos ent√£o perceber que o laborat√≥rio de Ci√™ncias √© um local onde os saberes de Qu√≠mica podem ser constru√≠dos e que a atividade por n√≥s realizada teve implica√ß√Ķes significativas para nossa forma√ß√£o como licenciandos em Qu√≠mic
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