2,857 research outputs found

    Byron Hawk, David M. Rieder, Ollie Oviedo (eds), Small Tech: The Culture of Digital Tools

    Get PDF
    Byron Hawk, David M. Rieder, Ollie Oviedo (eds), Small Tech: The Culture of Digital Tool

    Byron Hawk, David M. Rieder, Ollie Oviedo (eds), Small Tech: The Culture of Digital Tools

    Get PDF
    Byron Hawk, David M. Rieder, Ollie Oviedo (eds), Small Tech: The Culture of Digital Tool

    Microtubule disassembly delays the G2–M transition in vertebrates

    Get PDF
    AbstractWhen cell cultures in growth are treated with drugs that cause microtubules to disassemble, the mitotic index (MI) progressively increases as the cells accumulate in a C-mitosis. For many cell types, however, including rat kangaroo kidney PtK1 cells, the MI does not increase during the first several hours of treatment [1–3] (Figure 1). This ‘lag’ implies either that cells are entering mitosis but rapidly escaping the block, or that they are delayed from entering division. To differentiate between these possibilities, we fixed PtK1 cultures 0, 90 and 270 minutes after treatment with nocodazole, colcemid, lumi-colcemid, taxol or cytochalasin D. After 90 minutes, we found that the numbers of prophase cells in cultures treated with nocodazole or colcemid were reduced by ∌80% relative to cultures treated with lumi-colcemid, cytochalasin D or taxol. Thus, destroying microtubules delays late G2 cells from entering prophase and, as the MI does not increase during this time, existing prophase cells do not enter prometaphase. When mid-prophase cells were treated with nocodazole, the majority (70%) decondensed their chromosomes and returned to G2 before re-entering and completing prophase 3–10 hours later. Thus, a pathway exists in vertebrates that delays the G2–M transition when microtubules are disassembled during the terminal stages of G2. As this pathway induces mid-prophase cells to transiently decondense their chromosomes, it is likely that it downregulates the cyclin A–cyclin-dependent kinase 2 (CDK2) complex, which is required in vertebrates for the early stages of prophase [4]

    Highly sensitive micro coriolis mass flow sensor

    Get PDF
    We have realized a micromachined micro Coriolis mass flow sensor consisting of a silicon nitride resonant tube of 40 ?m diameter and 1.2 ÎŒm wall thickness. Actuation of the sensor in resonance mode is achieved by Lorentz forces. First measurements with both gas and liquid flow have demonstrated a resolution in the order of 10 milligram per hour. The sensor can simultaneously be used as a density sensor

    Controlled low-temperature molecular manipulation of sexiphenyl molecules on Ag(111) using scanning tunneling microscopy

    Full text link
    A novel scanning tunneling microscope manipulation scheme for a controlled molecular transport of weakly adsorbed molecules is demonstrated. Single sexiphenyl molecules adsorbed on a Ag(111) surface at 6 K are shot towards single silver-atoms by excitation with the tip. To achieve atomically straight shooting paths, an electron resonator consisting of linear standing wave fronts is constructed. The sexiphenyl manipulation signals reveal a pi-ring flipping as the molecule moves from hcp to fcc site. Abinitio calculations show an incorporation of the Ag atom below the center of a pi-ring.Comment: 4 pages, 4 figures, accepted for publication in Phys. Rev. Let

    “I’d Rather Be in Afghanistan”: Antinomies of \u3cem\u3eBattle: Los Angeles\u3c/em\u3e

    Get PDF
    This article reads Battle: Los Angeles (2011) against the grain to argue that the film possesses an antiwar undertow running unexpectedly counter to its surface-level pro-military politics. The article uses the antinomy structuring Battle: Los Angeles as the opportunity to explore the pro- and anti-war politics of science fiction alien invasion film more generally, as well as consider the role of cooperation with the military in Hollywood blockbusters. The article closes with a Jamesonian reading of “the army”: as a kind of utopia as registered by mainstream cultural texts like Battle: Los Angeles

    Combination of suicide and cytokine gene therapies as surgery adjuvant for canine mammary carcinoma

    Get PDF
    The incidence of canine mammary carcinoma varies with age, breed, and spay status, being among the main tumors appearing in intact female dogs. Thirty-six canine mammary carcinoma patients received injections of canine interferon-ÎČ (cIFN-ÎČ) and HSV-thymidine kinase/ganciclovir (HSV-tk/GCV) carrying lipoplexes, into the tumor bed, immediately after surgery. Next, they started periodic subcutaneous injections of lipoplexes carrying a human granulocyte-macrophage colony stimulating factor and interleukin-2mixedwith allogeneicmammary carcinoma extracts. This combined strategy was safe and well tolerated. In addition, only two out of 26 patients treated with complete surgery developed a local relapse, and 0 out of 29 stage II and III patients displayed distant metastases, suggesting both local and systemic antitumor activities. The most encouraging result was the long survival times: 22 > 1 year (where 13 > 2 and 4 > 3 years), while maintaining a good quality of life. The preliminary results in five patients presenting with local disease, an additional HSV-tk/GCV plus cIFN-ÎČ gene treatment induced local antitumor activity, evidenced by four objective responses (one complete, three partial) and one stable disease. This successful outcome supports further studies to validate this approach not only for canine veterinary patients, but also for translation to human patients.Fil: Finocchiaro, Liliana Maria Elena. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de OncologĂ­a "Dr. Ángel Roffo". Unidad de Transferencia GenĂ©tica; ArgentinaFil: Spector, Agustina I. M.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de OncologĂ­a "Dr. Ángel Roffo". Unidad de Transferencia GenĂ©tica; ArgentinaFil: Agnetti, Lucrecia. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de OncologĂ­a "Dr. Ángel Roffo". Unidad de Transferencia GenĂ©tica; ArgentinaFil: Arbe, MarĂ­a Florencia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de OncologĂ­a "Dr. Ángel Roffo". Unidad de Transferencia GenĂ©tica; Argentina. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas; ArgentinaFil: Glikin, Gerardo Claudio. Universidad de Buenos Aires. Facultad de Medicina. Instituto de OncologĂ­a "Dr. Ángel Roffo". Unidad de Transferencia GenĂ©tica; Argentina. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas; Argentin

    Gender-Specific Protection from Microvessel Rarefaction in Female Hypertensive Rats

    Get PDF
    Epidemiologic studies reveal that women have a significantly lower age-adjusted morbidity and mortality from cardiovascular disease than men, suggesting that gender is a cardiovascular disease risk factor. The mechanism of the “gender protection” is unknown. In this study, we investigated the microvascular remodeling in reduced renal mass plus a high salt (4.0% NaCl) diet model of hypertension (RRM + HS). We hypothesized that women would be protected from the increase in blood pressure and from the microvascular rarefaction associated with RRM + HS hypertension. Studies were designed to determine whether female rats were less susceptible to changes in microvessel density during RRM + HS. Microvessel density was measured in male and female low salt (0.4% LS) sham-operated controls (Sham + LS) and after 3 days or 4 weeks of RRM + HS hypertension. The microcirculation of hind limb (medial and lateral gastrocnemius, plantaris, soleus) muscles was visualized using rhodamine-labeled Griffonia simplicifolia I lectin. Tissue sections were examined by videomicroscopy and microvessel density was determined by quantitative stereology. As shown previously, mean arterial pressure increased to 160 ± 8 mm Hg and microvessel density decreased (\u3e30% decrease in all beds) in male RRM + HS. In contrast, mean arterial pressure of female RRM + HS rats was modestly increased from 101 ± 2 to 118 ± 4 mm Hg. Despite previous results showing a reduction in microvessel density of both normotensive and hypertensive male rats on a high salt diet, microvessel density of female RRM + HS rats was not reduced at either time. These results suggest that gender protection in the RRM rat extends beyond an attenuation of the increase in pressure to an immunity from microvascular rarefaction

    Science Fiction

    Get PDF
    Science fiction (SF) emerges as a distinct literary and cultural genre out of a familiar set of world-famous texts ranging from Mary Shelley’s Frankenstein (1818) to Gene Roddenberry’s Star Trek (1966–) to the Marvel Cinematic Universe (2008–) that have, in aggregate, generated a colossal, communal archive of alternate worlds and possible future histories. SF’s dialectical interplay between utopian optimism and apocalyptic pessimism can be felt across the genre’s now centuries-long history, only intensifying in the 20th century as the clash between humankind’s growing technological capabilities and its ability to use those powers safely or wisely has reached existential-threat propositions, not simply for human beings but for all life on the planet. In the early 21st century, as in earlier cultural moments, the writers and critics of SF use the genre’s articulation of different societies and different possible futures as the occasion to reflect on our own present, in ways that range from full-throated defense of the status quo to the ruthless denunciation of all institutions that currently exist in the name of some other, better world. SF’s global popularity has grown to the point where it now looms quite large over cultural production generally, becoming arguably the most popular narrative genre in existence, particularly in the sorts of SF action spectacles that have dominated the global box office of the first two decades of the 21st century. It has also become increasingly difficult to tell the difference between the things we used to think of as SF and the advanced communication, transportation, and entertainment technologies that have become so ubiquitous and familiar that we now take them for granted, as well as the growing prevalence of political, economic, and ecological crises now erupting out of the pages of our science fictions, like our very worst dreams come to life
    • 

    corecore